Dna micronutritients menopause dez 2012 kopie
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Martin ImhofLandesklinikum Korneuburg/Vienna
Mikronährstoffe und ihr therapeutischer Effekt auf genetischeAberrationen und DNA am Beispiel
der Spermienqualität“
Menopause, Andropause und Antiaging. Wien Hotel Hilton 2012
Semen quality and Sperm DNA damagein relation to urinary bisphenol A .
Bisphenol A
John D. Meekera et al. Reproductive Toxicology 2010
Swan et. al. – National Institute of environmental health sciences
•Review of 61 published studies•Data collected over 58 years (1938 to 1996)•Decline in average sperm density in U.S. and other Western countries
Average annual decrease of
U.S. 1.5 mio/ml = minus 1.5%/year
Europa minus 3.1%/year
Rückgang der Spermienqualität
Ungeklärte Fertilitätsstörungen?
Effekte der DNA Fragmentation
Fertil Steril. 2002 Aug;78(2):313-8.
Saleh ED; Increased sperm nuclear DNA damage in normozoospermic infertile men: a prospective study.,
CONCLUSION(S): ….. a significant increase in DNA damage can be found in sperm from infertile men with normal standard sperm parameters …………
Effekte der DNA Fragmentation
PLoS One. 2012;7(9):e44679
Ribas-Maynou J, Double stranded sperm DNA breaks, measured by Comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor.
Conclusion: ….. sperm DNA breaks are related to the paternal risk of having recurrent pregnancy loss …..
Effekte der DNA Fragmentation
Fertil Steril. 2007 May;87(5):1077-86.
Sloter ED Frequency of human sperm carrying structural aberrations of chromosome 1 increases with advancing age.
CONCLUSIONS: Our findings suggest that advancing male age is associated with a gradual and significant increase in the risk of fathering children with various chromosomal defects such as segmental aneusomy syndromes.
DNA Fragmentation
einer oder mehrere Brüche einer oder beider Stränge der Doppelhelix
Effekt der DNA Fragmentation
Spermien haben kein eigenes DNA-Reparatursystem, Schäden können im Gegensatz zur Oozyte nicht repariert werden
DNA-Fragmentation von Samenzellen im kaudalen Nebenhoden und im Ejakulat am höchsten da höchste Radikalbelastung (ROS)
DNA Fragmentation bewirkt Störung der Kapazitation und Abbruch in der Frühentwicklungsphase des Embryos
J Assist Reprod Genet. 2009 Aug;26(8):427-32.
Zini A, Antioxidants and sperm DNA damage: a clinical
perspective.
RESULTS: . the data suggest that dietary antioxidants may be
beneficial in reducing sperm DNA damage, particularly, in men
with high levels of DNA fragmentation ...
EFFECT OF MICRONUTRITIENTS on DNA
Gibt es einen Mangel an Mikronährstoffen?
Guidelines on food fortification with micronutrientsLindsay Allen et al. World Health Organization2006
Male factor subfertility: possible causes and theImpact of nutritional factors. Wong WY et al. Fertil Steril 2000 73:435-442
Gibt es einen Mangel an Mikronährstoffen?
Gibt es einen Mangel an Mikronährstoffen?
DECREASE IN SPERM QUALITY
Dietary intake of antioxidant nutrients is associated with
semen quality in young university students
Mínguez-Alarcón, Hum Reprod. 2012 Sep;27(9):2807-14
In this cross-sectional study, 215 young men
Our study suggests that some sperm parameters are sensitive
to dietary intake of antioxidant nutrients
2x1 Capsule daily for 3 Month
L-Carnitin 440 mg L-Arginine 250mg Vitamin E 120 mg Folic-acid 800 μg Zinc 40 mg Selenium 60 μg Glutathione 80 mg Coenzyme Q10 15 mg
Study Medication „PROFERTIL“
EFFEKTE auf die SPERMIOGENESE
Hormonal Regulation
Morphology/Density
Energy/Motility
Enzym/Capazitation
L-Carnitin 440 mg
L-Arginine 250 mg
Vitamin E 120 mg
Folic-acid 800 ug
Zinc 40 mg
Selenium 60 ug
Glutathione 80 mg
Coenyme Q 10 15 mg
Therapy Group 132 men, age Ø 34 a (18-43)
2 Capsules/day MICRONUTRITIENTS (PROFERTIL) for 3 month
Control Group 73 men, age Ø 38 a (22-52)
No TREATMENT / CHANGE OF LIFE STYLE
CONTROLLED STUDY – STUDY SETTING
THERAPY COMPARED TO CONTROL
6 month after end of therapy
Study Group Control Group
132 74
34 preg.(25,8%) 11 preg.(14,8%)
Improvement with Micronutritients after 3 Month
Mikronährstoffe und DNA Fragmentation
Cochrane Database Syst Rev. 2011 Jan 19
Showell et al., Antioxidants for male subfertility.
AUTHORS' CONCLUSIONS: The evidence suggests that antioxidant supplementation in subfertile males may improve the outcomes of live birth and pregnancy rate for subfertile couples undergoing ART cycles….
Assist Reprod Genet. 2009 Sep-Oct;26(9-10)
Tunc O, J Oxidative DNA damage impairs global
sperm DNA methylation in infertile men.
CONCLUSIONS: ..... while antioxidant supplementation
appears to have the potential to reduce DNA damage and
normalize sperm DNA methylation.
EFFECT OF MICRONUTRITIENTS on DNA
Therapy Group 67 men, age Ø 39 a (24-48)
2 Capsules/day MICRONUTRITIENTS (PROFERTIL) for 3 month
Control Group 40 men, age Ø 37 a (26 – 50)
No active TREATMENT
Main outcome measure was change in sperm-hyaluronan
binding assay indicating DNA fragmentation after 3 months.
SPERM DNA FRAGMENTATION INDEX DECREASES AFTER MICRONUTRIENT SUPPLEMENTATION
Inclusion – criteria
Infertility for > 1 years 2 pathological spermiograms Informed consent
Exclusion - criteria
VaricosisInfectionAspermiaHormonal disorder
CONTROLLED STUDY – STUDY SETTING
KontrolleKeine Therapie
20 %
40 %
100 %
80 %
60 %
Ergebnisse
Therapie Micronährstoffen Profertil
0 %
56%
69%
20 %
40 %
100 %
80 %
60 %
0 %
74%
64%
(p<0.05) (p=0.083).
EFFECT OF MICRONUTRITIENTS on DNA
Reprod Biomed Online. 2009;19 Suppl 3:35-43.
Tarozzi N Sperm-hyaluronan-binding assay: clinical value in conventional IVF under Italian law.
…. The results underline the ability of hyaluronan to select spermatozoa with higher DNA integrity and morphology ….
DNA Fragmentation
sperm chromatin structure analysis
< 15% normal 15-30% IUI necessary> 30% ICSI necessary
Zusammenfassung
Oxidative Prozesse verursachen Fertilitätsstörungen
Freie Radikale führen zu Brüchen in der Spermien DNA
Mikronährstoffe können als Radikalfänger auftreten
Mikronährstoffe verringern die DNA Fragmentationsrate und erhöhen die Hyluronsäurebindung
Mikronährstoffe beeinflussen positiv die Schwangerschaftsrate und den Schwangerschaftsverlauf
Vielen Dank für Ihre Aufmerksamkeit