Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the...

56
1 Bundesamt für Bevölkerungsschutz BABS LABOR SPIEZ Weiter- und Fortbildungs-Symposium Molekulare Diagnostik 5. März 2015 Dr. Marc Strasser, Head Virology, SPIEZ LABORATORY Ebola outbreak and the SPIEZ LABORATORY

Transcript of Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the...

Page 1: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

1

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Weiter- und Fortbildungs-Symposium

Molekulare Diagnostik 5. März 2015

Dr. Marc Strasser, Head Virology, SPIEZ LABORATORY

Ebola outbreak

and the SPIEZ

LABORATORY

Page 2: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

2

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

• The Ebola Virus

• Transmission

• Ebola outbreak - current

situation in westafrica

• Control strategies

• Tasks of SPIEZ LABORATORY

• SPIEZ LAB field mission (EmLab) in Westafrica

Content

Page 3: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

3

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

The Ebola Virus

• Filovirus (Filoviridae)

• Variable relevant spezies (Ebola, Marburg, Reston)

• Incubation time: 2 - 21 days

• Letality: 30 - 90%

• Transmission: through body fluids

• Symptoms: diarrhea, vomiting, head- and bodypains, fever,

rash, inner and external bleedings

Page 4: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

4

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

The Ebola Virus

Properties:

• Filoviruses are filamentous

Viruses (latin: filum)

• Envelopped,

single stranded-RNA-Viruses.

• Length between 1‘000 and 14‘000 nm, dimeter 60 to 80 nm

• 1967: First cases of Filovirus (Marburg) in Europe.

• 1976: First Ebola outbreak (Ebola River in what is now the

Democratic Republic of the Congo)

Befällt die meisten Zellen eines Organismus

100 nm

Page 5: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

5

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

The Ebola Virus

Replikation cycle:

May infect and propagate in most of the human tissue

100 nm

Page 6: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

6

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

The Ebola Virus

• Filovirus (Filoviridae)

• Variable „human relevant“ spezies (Ebola, Marburg)

• Incubation time: 2 - 21 days

• Fatally rate (lethality): 30 - 90%

• Transmission: through body fluids

• Symptoms: diarrhea, vomiting, head- and bodypains, fever,

rash, inner and external bleedings

Page 7: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

7

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

The Ebola Virus

• 2007

Uganda

• 37 deaths

Page 8: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

8

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

The Ebola Virus

• Filovirus (Filoviridae)

• Variable relevant spezies (Ebola, Marburg, Reston)

• Incubation time: 2 - 21 days

• Letality: 30 - 90%

• Transmission: through body fluids (actually !!)

• Symptoms: diarrhea, vomiting, head- and body pains, fever,

rash, inner- and external bleedings

Page 9: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

9

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Transmission (Reservoir)

• Fruit bats (Rousettus aegyptiacus) ,

living in the tropical rain forest are in

fact the reservoir for the Ebola Virus

(published in 2007).

• They carry the pathogen without

getting ill.

• Fruit bats segregate the pathogen

through urine and saliva.

Page 10: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

10

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Transmission (other Spezies)

• Fruit bats are licking and chewing

fruits and remains of them will be

splitted out.

• Such scraps of fruit are eaten by eg.

primates, antilopes and boars.

• Transmission and infection occurs

through little lesions and wounds in

mouth or skin of these animals.

• Mostly of such infected animals get

seriousely ill and subsequently die.

Page 11: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

11

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Transmission (by Bushmeet)

• Il or dead animals (bushmeet)

are collected by the poor

population, cutted in multiple

bloody pieces and shared with

family or clan members.

Page 12: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

12

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Transmission (rituals)

• Rituals are very widespread

in the westafrican region.

• Ill persons or dead bodies

are touched, washed, kissed

and embrassed without any

protection.

• This mode of behaviour

promotes the transmission of

the Ebola Virus through

body- and fluid contact and

represent therefore a

huge risk of infection.

Page 13: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

13

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Transmission (Cycle)

Page 14: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

14

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Ebola outbreak in Africa

Page 15: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

15

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Ebola outbreak in Africa

Page 16: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

16

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Distribution in 2014

June July August

Ebola outbreak in Africa

The indicator case was already registered in September

2013 but not recognized as an Ebola infection.

Page 17: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

17

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Situation as of February 2015

Source: CDC, Atlanta, USA

Page 18: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

18

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Situation ausserhalb Westafrikas

6.Oktober 2014

Page 19: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

19

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Total cases February 2015

Page 20: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

20

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Guinea (66.7% )

Page 21: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

21

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Sierra Leone (29%)

Page 22: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

22

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Liberia (42%)

Page 23: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

23

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

First isolates from Ebolastrain Zaire (Guinea)

Page 24: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

24

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Marburg outbreak

Page 25: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

25

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Control strategies

National (in west africa):

• Build up of medical hospital wards.

• General education concerning the disease

• Build up medical care

• Military operation

International missions:

• Medical care people (MSF)

• Build up Laboratory capacity (eg. EmLab)

Page 26: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

26

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Current Countermeasure strategies

Vaccines:

Multiple variants are succsessfully tested on NHP ‘s.

Two of them are actually tested on volunters in Switzerland (HUG,

CHUV).

• In future vaccines can be applied prophylactic.

• The application must be comprehensiv (a whole population

should be vaccinated)

Antibodies:

Artificially produced antibodies (eg. ZMapp) or reconvalescent

serum (from survivors) are promising if applied in an early stage of

infection.

Antiviral Substances:

Existing effective chemical substances against viral infections (eg.

Interferon, Tamiflu, Ribavirin).

• Antibodies and antivirals may be used in an early stage of

infection (as soon as possible).

Page 27: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

27

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Vaccine trials 1

Page 28: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

28

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Vaccine Trials 2

Page 29: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

29

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Tasks of SPIEZ LABORATORY

Biocontainment Laboratory

BSL-4

Page 30: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

30

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

SPIEZ LABORATORY

Swiss Institute for NBC Protection and NBC Therats.

Mission of the biology branche (1995 – to date):

Detection of special highly pathogenic human microorganisms

and toxins as well as detection of potentially warefare Agens

Page 31: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

31

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Birds view

Page 32: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

32

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Organigram

Page 33: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

33

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Biology branch: Main Functions and Tasks

Diagnostics

- Primary diagnostics for RG3, RG4

- Differential diagnostics

- Analysis of unknown samples → CBRN

Research

- Basic and applied science

Training

- Provider for Biosafety / Biosecurity

Reference Function

- WHO Collaborating Center (Biosafety)

- National Reference Center VHF

Page 34: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

34

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Biology

Bakteriology

Virology

Toxinology

- Watercontrol for the

Swiss Army

- Scientific Projects

with external Partners

- Detection and

diagnostics of RG 3

and RG 4 viruses

- Detection and

diagnostics of RG 3

bacteria

- Detection and

diagnostics of

biological toxins

- Diagnostics in

molecular biology

- Development and

validation of methods

Page 35: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

35

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Project Biocontainment Laboratory

1996 Initiation of the Project

2001 Site Selection

2003 Political Commitment (DDPS)

2005 Enviromental Impact Study > Building permit

2006 Submit Project to Parliament > Financial Support

2005 (28.5 Mio CHF)

2007 Start Construction

2012 Initial Operation

(BSL-2, BSL-3 Labs)

2012 – 2013 Integral testing and

validation

2014 Operation of the BSL-4 Lab

Page 36: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

36

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Risk groups and Biosafety levels

RG 1: no risk (Bakers yeast) → BSL 1

RG 2: slight risk (Salmonella) → BSL 2

RG 3: moderate risk (Anthrax) → BSL 3

RG 4: high risk (Ebola, Smallpox) → BSL 4

Risk groups RG Biosafety Levels BSL for microorganisms for Laboratories

Biocontainment laboratory Spiez

Page 37: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

37

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

The biocontainment laboratory allows the safe handling of pathogenic micro-

organisms in the highest risk groups (RG 3 and 4).

Safety is the number one priority in the biocontainment laboratory to the aim

that the public and the environment are protected from release of infectious

pathogens and that the staff is protected from lab associated infection.

Biosafety related tasks include:

• Guaranteeing biological safety

• Guaranteeing technical safety

• Training

Biocontainment Laboratory

Page 38: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

38

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Containment Concept

Page 39: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

39

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Level 4 Laboratory

Primary Barriers Secundary Barriers

All works in MSC III

or in MSC I + II benches in

Combination with protective

Suits under overperssure

> Protection of Staff

- tight Construction

- Locker Systems

- Double door access

- Underpressure

- Air filtration (HEPA)

> Protection of the Enviroment

Page 40: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

40

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Biocontainment Laboratory: Design

Page 41: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

41

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

The lab staff is protected from the pathogens

Biocontainment Laboratory

Page 42: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

42

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Society / environment is protected from pathogens

Filtration of incomig and outgoing air

using HEPA filters (H14)

Waste water treatment (sterilisation)

Biocontainment Laboratory

Page 43: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

43

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Inactivation of patient samples

in BSL3+ (Glovebox) or BSL4 lab

• Inactivation of whole blood & serum in

extraction reagent (AVL-buffer)

• Sluice out inactivated material

• time required: 2-3 h

RNA extraction: time required: 1 h

Mastermix and amplification RT-PCR:

• time required: 2 h

Time for total primary diagnostic approx 6 h

Confirmatory tests: ->

Ebola Virus Diagnostic: Laboratory Requirements

Page 44: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

44

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

End-point RT-PCR with sequence analysis

• time required 2 days

Virus isolation in cell cultures

• from whole blood, serum, urine,

clinical and environmental swabs

• time required 7-9 days

Further characterization:

• Whole genome sequencing

->Methods are being established

• Electron microscopy

Ebola Virus Diagnostic: Confirmation Tests &

further analysis

Page 45: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

45

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Objective:

Dose finding, safety and

immunogenicity phase I/II vaccine

study

• Recombinant Vesicular Stomatitis

Virus in which envelope

glycoprotein (GP) is replaced by

Zaire Ebola virus GP.

• In vivo replication is limited in

NHP, but release of large amounts

of ZEBOV GP.

• No serious adverse effects

observed with VSV-ZEBOV

Ebola vaccine phase l/II trial

Page 46: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

46

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Dose finding

(single application)

Vaccinees from

Switzerland (Geneva),

Gabon, Kenia

Analysis:

Clinical data

Immunological readout

Our Role (Spiez Lab):

Assessment of neutralization capacities in approx. 150 vaccines, serum

from 2 time points -> 300 serum to test in the BSL-4 Lab

VSV EBOVAC phase I/II dose finding trial

Page 47: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

47

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Establishment of Mobile Laboratories for Pathogens up to Risk Group 4

in Combination with CBRN Capacity Building in Sub-Saharan Africa"

Funded by the European Comission

Build the capacity to bring state-of-the-art technology, diagnostics and highly trained scientists

from Europe and Sub-Saharan African countries to the field in case of infectious disease

epidemics in Europe and Sub-Saharan African countries.

http://www.emlab.eu

• Bernhard-Nocht-Institute for Tropical

Medicine, Hamburg, Germany

• Institute for Lassa Fever Research and

Control (ILFRC), Irrua Specialist Teaching

Hospital, Irrua, Nigeria

• National Institute for Medical Research,

Dar es Salaam, Tanzania

• Bundeswehr Institute of Microbiology,

Munich, Germany

• Istituto Nazionale per le Malattie Infettive

"L. Spallanzani", Rome, Italy

• Institute for Virology, Philipps-University

Marburg, Marburg, Germany

• Laboratoire P4 Inserm Jean Merieux,

Lyon, France

• Spiez Laboratory, Spiez, Switzerland

• Public Health England, Microbiology

Services Division-Colindale, London, UK,

and Microbiology Services-research

Salisbury, UK

Field mission with the European Mobile Laboratory

(EMLab) and MSF in May/June 2014

Page 48: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

48

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

• Modular and rapidly deployable

mobile laboratory in 15 boxes

(checked baggage)

• 3 Lab Units (Tanzania, Nigeria,

München)

• Diagnostic of P3 und P4-

pathogens by PCR, antibody

detection and rapid tests

• Regular trainings und workshops

• Deployable for outbreak

missions

EMLab Concept

Page 49: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

49

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Transfer to Guéckédou

Page 50: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

50

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Hospital tent and medical care workers

Page 51: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

51

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Transfer of Patients to the hospital

Transportation of

infected persons !!

Decontamination of

cars and car

equipment !!

Bad protection

Page 52: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

52

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

MSF protective equipment for patient care

Page 53: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

53

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Sampling by MSF and transfer to the EMlab

MSF Camp (overview)

Page 54: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

54

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

RNA extraction & Mastermix prep PCR amplification (SmartCycler)

Inactivation of patients samples in the glovebox

Sample inactivation by Jasmine Wastebag

Page 55: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

55

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

MSF Logistic

Preparation of the desinfection

solution (Chavel)

Page 56: Ebola outbreak• The Ebola Virus • Transmission • Ebola outbreak - current ... Mission of the biology branche ... Project Biocontainment Laboratory 1996 Initiation of the Project

56

Bundesamt für Bevölkerungsschutz BABS

LABOR SPIEZ

Bild: «biological weapons»

Heinz Sager, 2002

www.labor-spiez.ch

[email protected]

Thank you very much for your attention