HAI Price List 2005 - HPLC Column Manufacturer
Transcript of HAI Price List 2005 - HPLC Column Manufacturer
HigginsAnalytical
EXPERT MANUFACTURER OF HPLC CONSUMABLE S
2013
WORLDWIDE AVA I LAB I L I TY AND SUPPORT
Higgins Analytical ist eine Qualitäts orientierte Firma,welche sich der Lieferung von leistungsfähigenProdukten widmet, Kundenunterstützung durchExperten, sowie rasche Lieferung an Forschungs undQualitätskontrolleinrichtungen ist weltweit unser Ziel.
La Higgins Analytical é una societá orientata versol’alta qualitá, dedicata a fornire ai laboratori diricerca e controllo qualitá nel mondo prodotti adalto livello, supporto di esperti e rapide consegne.
Higgins Analytical is a quality oriented company, dedicatedto providing high performance products, expert support, andrapid delivery to research and quality control laboratoriesworldwide.
Higgins Analytical es una compañia dedicada a poveerproductos de óptima calidad, excelente rendimiento,experto soporte técnico y entrega immediata a los lab-oratorios de investigación y de control de calidad delmundo entero.
I Higgins Analytical iyinkampani engusaziwayo kwe-zobuchwepheshe. Izinikele ekuthini ikhiqizeumkhiqizo oseqophelweni eliphezulu. Izebenzisauxhaso oluphambili ukukhipha imiphumela ecut-shungwe kahle nangokushesha kanye nezindawozokucubungula eziseqophelweni eliphezulu emh-labeni jikelele.
Higgins Analytical est une société ayant pourobjectif la qualité et de proposer des produitsperformants, un support expert, des livraisonsrapides pour les laboratories de recherches etde contrôle qualité à travers le monde.
Higgins Analities is ‘n kwaliteits-georienteerdemaatskappy wat toegewyd is om produkte vanhoogstaande gehalte, kundige ondersteuning ensnelle lewering van produkte vir navorsings-engehalte-beheer laboratoria wereldwyd te voorsien.
Higgins Analytical on kromatografiakolonneja valmistava yritys, joka tarjoaa laadukkaita tuotteita, asiantuntevaapalvelua ja nopeat toimitukset tutkimus- ja laadunvalvon-talaoratoriolle maailmanlaajuisesti.
Higgins Analytical, hedefi yüksek performanslı ürünlersağlamak olan, dünya genelinde araştırma ve kalitekontrol labratuvarlarına expert destek veren ve hızlıdaḡıtım yapan kalite odaklı bir şirkettir.
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HIGGINS ANALYTICAL 2 - 3
CLIPEUS™ 4 - 5
HAISIL 100™ 6 - 7
HAISIL 300™ 8 - 9
HAISIL HL™ 10 - 11
HEAVY™ 12
Some Interesting Facts 13
PROTO™ 200 14 - 15
PROTO™ 300 16 - 17
PHALANX™ 18 - 19
TARGA® 20 - 21
Specialty Columns 22
Par t Number Guide 23
HPLC COLUMNS 24 - 29
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CONTENTS
MICROBORE COLUMNS 30 - 35
SEMI-PREP Columns 36 - 39
Caπllary™ NEW 40 - 41
Capellini™ 6-32 42 - 49
SPRITE™ 50 - 51
PICCOLO™ 52 - 53
s.s. Analytical Car tridges 54 - 57
PEEK/Titanium Car tridges 58 - 59
10mm Car tridge Columns 60 - 61
Analytical Guard Car tridges 62 - 63
Prep Guard Car tridges 64 - 65
Car tridge Holders 66 - 67
H HAIFILTER NEW 68 - 69XX-XX-XXX XX-XX-XXXX XX-XXXX-X
π 75µm and 150µm Columns
and PREP Columns
Ordering, Warranty, Trademarks 70 - 71
NEW 30x1.0mm for more capcity
NEW 35x20 & 50x20mm sizes
and Services
Par ts and Accessories
Capellini™ 10-32 NEW
H IGG INS ANALYT ICAL
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Experience, Expertise, ContinuityHiggins Analytical was founded in early1994, but that is only half of the story.The two founders had already beenpacking commercial HPLC columns formore than fifteen years before that.Besides a lot of expertise, our experi-ence brings an unrivaled continuity tothe field.
Total Quality Approach to allAspects of Design, Manufacturingand Testing Each HPLC column and analyticalcartridge from Higgins Analytical isevaluated by a computerized perform-ance test prior to receiving a quality
control approval. This testing protocolinvolves a highly accurate statisticalmoments determination of the column’sperformance characteristics. Thesesensitive peak shape and columnefficiency measurements can only bemade by computerized data loggingmethods. The raw digital chromatogramfor every column we have manufacturedsince March 1994 is retrievable from ourarchives.
Product TraceabilityThe test data for these serializedcolumns include sorbent properties andlot number as well as complete descrip-tion of the test conditions. We are ableto trace the serial number of every
column to the customer that originallypurchased the column.
Comprehensive Record Keepingand Statistical Quality ControlRaw digital test data is automaticallyarchived for every column and analyticalcartridge we manufacture. As a result, itis a simple matter for us to retrievecomprehensive quality control informa-tion and routinely apply it to ourstatistical process control activities. This
commitment to quality manufacturingand comprehensive record keepingpermits us to produce replacementCertificates of Analysis when requestedby laboratories complying with theirinternal GLP needs. And, most
importantly, we are able to assistcustomers during the lengthy processbeginning with method development andoptimization to validation and NDA filingby providing comprehensive serialnumber, manufacturing date, sorbent lot,and performance data for every columnthey ever purchased.
Expert Manufacturing and State-of-the-Art Equipment Two of Higgins Analytical’s staff havebeen involved with the chemical separa-tions business for over 30 years, othersfor only 10 or 15. By focusing thisexpertise exclusively on the separationsconsumables business, high qualityproducts as well as high quality processesare assured. A key element of thisexpertise is the electronic manufacturing
lab book that we have developed. All ofour column packing equipment iscontrolled by this electronic book, and allmethods are electronically stored. It isimpossible to pack a column without theelectronic lab book being completelydocumented. Processes cannot be
Quality and Environmental Aspectsof Process SolventsProper composition of slurry and pack-ing solvents is vital to the whole process.Our solvent purification equipment andprocedures allow us to address all of thesolvent requirements for production andquality control. Not only does thisinvestment permit us to use the highestpurity solvents available, it also eliminatesmuch of the expensive and environment-polluting solvent disposal practice char-acteristically found in today’s chemicalindustry.
Unique Bonded Phase SynthesisChemists at Higgins Analytical have beenproducing bonded phases since 1979.During this long experience, we havepioneered many technologies longbefore they were “discovered” by otherswith a bigger marketing clout. HigginsAnalytical’s introduction of a “No
Requirement for TFA” HPLC column inearly 1997 is just one example of ourvanguard approach to this field.
Computer Aided Drawings andNumerical Control MachineryKey to reinforcing our ability to providethe highest performance-to-price ratioproducts available in the separationsconsumables industry has been ourinvestment in computer aided design(CAD) technology and computernumerically controlled (CNC) machinery.SolidWorks™ software is used by ourengineers which permits us to designHPLC hardware and see it in its
PROCES S AND PRODUCT CONTROL
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changed without an expert supervisor’sintervention. When new processes fornew products are developed oroptimization is required for an existingmethod, a complete audit trail is createdfor every condition change that is made.The packing pumps operate with PID(proportional integral/derivative)controllers allowing for control flow rate,
rather than pressure, throughout thepacking process. This is a very importantpoint. Common folklore is that it takeshigh pressure to pack an HPLC column.Actually, pressure is only the result of a
complex two phase (liquid/solid) flow.This flow, not pressure, is what producesa packed bed. The “pressures” used bymany column manufacturers are theresult of flow rates that approach thevelocities that will produce a “fluidizedbed” during certain phases of the packingprocess, not exactly a condition that willproduce a stable, optimally packedcolumn. In addition to being able tocontrol our equipment digitally, we areable to data log the actual conditions aswell.
Our unique and highly automatedcolumn packing process is based on aclosed, continuous flow system employingrecoverable, unreactive, and safe fluids.And the best part about it is that itenables Higgins Analytical to manufacturea wide variety of HPLC columns withunrivaled efficiency.
assembled 3D form before we manufac-ture it. Dedicated tooling and securebackups of machine code assure fastsetups and consistant high qualityproduction of all the stainless steel andplastic components in each product line.
Our product development, manufac-turing, and testing processes strictlyfollow a systematic and welldocumented cycle.
A Certificate of Analysis, like the one below for a3cm x 10mm cartridge, accompanies each
HPLC Column and Cartridge we manufacture
Note: This high performance cartridgeis typically used as a guard column.
CL I P EUS
CLIPEUS A “Small Shield” for a Big Job Phases Silica, C8, C18, Cyano, Phenyl
Particle Sizes 4.5 and 10µmPore Size 120Å
Pore Volume 0.8mL/gmSurface Area 330m2/gm
Carbon%(w/w) C18 = 18%Phase type Extremely well end-capped
monofunctional phasesSilica Class Type B
ApplicationsThe high purity base silica and thoroughlyend capped bonded phases insure thatCLIPEUS columns and cartridges will per-form well in demanding situations wheremany other column brands fail. Evenpolar compounds elute as sharp and sym-metrical peaks permitting surprisinglyshort columns to be used for high speedanalysis. In addition to Silica, C8 and C18phases, CLIPEUS columns are availablewith phenyl or cyano functionality.
Methylxanthines
CLIPEUS C18 150x4.6mm P/N CS-1546-C185
25% MeOH/water (unbuffered)1mL/min, 30ºC, 254nm
1. Theobromine 2. Theophylline3. Caffeine 4. Phenol
Carbamates and Urea PesticidesEPA 632A
Industrial and Municipal Waste WaterColumn P/N CS-2546-C185
1. Monuron 2. Propoxur 3. Carbaryl 4. Propham5. Diuron 6. Linuron 7. Methiocarb 8. Mexacarbate 9. Chlorpropham 10. Barban 11. Neburon
This unbuffered Pyridine and Phenol chromatogram illus-trates the absence of any undesirable solute/silanol inter-action on a CLIPEUS C18 HPLC Column.
CLIPEUS C18 5µm 150x4.6mm
40% MeOH/water (unbuffered)1mL/min, 25ºC, 254nmP/N CS-1546-C185
Sunscreen Formulation
CLIPEUS C18 250x4.6mm P/N CS-2546-C185
87.5% MeOH/ 12.5% water (unbuffered)1mL/min, 30ºC, 285nm
1. Oxybenzone 2. Padimate O3. Octyl Methoxycinnamate
Guide to CLIPEUS Part NumbersCx-xxxx-SIL5 CLIPEUS Silica 5µmCx-xxxx-C085 CLIPEUS C8 5µmCx-xxxx-C185 CLIPEUS C18 5µmCx-xxxx-CNP5 CLIPEUS Cyano 5µmCx-xxxx-PHN5 CLIPEUS Phenyl 5µmSee Page 23 for complete Part Number information
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Comparative Selectivity of CLIPEUS HPLC Columns
Neutrals Test Mix NIST Test Mix
1. Uracil 2. Toluene 3. Ethylbenzene4. Quinizarin 5. Amitriptyline
1. Thiourea 2. Phenol 3. Acetophenone4. Nitrobenzene 5. Toluene 6. Napthalene
CLIPEUS C18 150x4.6mm P/N CS-1546-C185
CLIPEUS C8 150x4.6mm P/N CS-1546-C085
CLIPEUS Phenyl 150x4.6mm P/N CS-1546-PHN5
CLIPEUS Cyano 150x4.6mm P/N CS-1546-CNP5
50% MeCN/water, 1.5mL/min, 205nm 80% MeOH/Buffer, 1.5mL/min, 254nmBuffer: 20mM potassium phosphate
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CL I P EUS APPL ICAT IONS
HA I S I L 1 0 0
HAISIL 100 The WorkhorsePhases Silica, C8, and C18
Particle Sizes 3µm and 5µmPore Size 100Å
Pore Volume 0.5mL/gmSurface Area 190m2/gm
Carbon%(w/w) C8 = 7%(w/w), C18 = 12%(w/w)Phase type Monofunctional & fully endcappedSilica Class Type A
ApplicationsHAISIL 100 columns and cartridges arevery good general purpose columns thatare particularly well suited for small mol-ecules and peptide applications. TheHAISIL 100 column and cartridge effi-ciencies are unrivaled yet they representone of the most economical productlines in the market today. In formatsranging from 0.075 to 20mm ID andfrom 10 to 250mm in length,
100Å vs. 300Å Columns for PeptideMapping The question of whether to use a 100Åor 300Å column for mapping usuallydivides peptide chromatographers intotwo groups. Rather than take sides, wehave chosen to offer very efficientcolumns with both pore diameters tosuit both camp's preferences. HAISIL100 and HAISIL 300 columns are avail-able in 10 to 250mm lengths and0.075mm to 20mm internal diameters.
Tryptic Map of Transferrin on HAISIL 100250x4.6mm P/N HS-2546-M185
Tryptic Map of Transferrin on HAISIL 300250x2.1mm P/N HS-2521-W185
Guide to HAISIL 100 Part NumbersHx-xxxx-SIL3 HAISIL 100 Silica 3µmHx-xxxx-M183 HAISIL 100 C18 3µmHx-xxxx-SIL5 HAISIL 100 Silica 5µmHx-xxxx-M085 HAISIL 100 C8 5µmHx-xxxx-M185 HAISIL 100 C18 5µmSee Page 23 for complete Part Number information
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Enzymatic Reaction Mixtures of Human 15-lipoxygenase
HAISIL 100 C18 250x4.6mm74.9% MeOH, 25% water, 0.1%HoAC
1mL/min, 234nmP/N HS-2546-M185
Economical Alternative for PICOTAG Analysis
Our HAISIL 100 C18 columns havedemonstrated superior resolution and lifetime for PICOTAG™ amino acid analysiswhen compared to considerably moreexpensive columns from other manufac-turers. Besides good linearity and columnlifetime, the 150x4.6mm column yieldsvery efficient amino acid analysis asshown in the chromatogram above (P/NHS-1546-M185).
ConditionsHAISIL 100 C18 5µm 150x4.6mmSolvent A: Phosphoric acid, ammonium
sulfate aqueous bufferSolvent B: Acetonitrile. Linear gradient at 1mL/min254nm detection
Analogs:A1: pGlu-His-Trp-Ser-Tyr-(d-Ser(but))-Leu-Arg-Pro-NHEtA2: pGlu-His-Trp-Ser-Tyr-d-Ala-Leu-Arg-Pro-NHEtA3: pGlu-His-Trp-Ser-Tyr-d-Leu-Leu-Arg-Pro-NHEtA4: pGlu-His-Trp-Ser-Tyr-d-Ala-Trp-Leu-Pro-NHEtA5: pGlu-His-Trp-Ser-Tyr-d-Trp-Leu-Arg-Pro-NHEt
Hormone Analogs LH-RH (Luteinizing Hormone - ReleasingHormone) Analogs are used for theinhibition of gonadal steroid production.An important application area is in thetreatment of prostate cancer.
Kinetic Measurements in DiseaseResearch Researchers at the University ofCalifornia, Santa Cruz have recentlypublished a novel HPLC method thatyields baseline separation of per-deuter-ated and protonated lipoxygenases.
The striking ability of a 250x4.6mmHAISIL 100 C18 column to resolvethese two very similar compounds isenabling researchers to study largecompetitive kinetic isotope effects inHuman 15-lipoxygenase catalysis.Protio/per-deutero ratio data obtainedby this HPLC method agreed withinexperimental error of data obtained byES-MS.
The elucidation of these mechanisms isnoteworthy because of the increasingrole human lipoxygenases are playing ininflammatory diseases and cancergrowth regulation.
Alkaloids A Peruvian vine named “Una de Gato”(Uncaria tomentosa) and commonlyreferred to as Cat’s Claw, has become apopular natural product source forherbal remedies. Enthusiasts claim theoxindole alkaloid extracts to be effectivein the treatment of a wide range ofdigestive and circulatory diseases as wellas other afflictions ranging from cancerto arthritis. Reputable providers of suchphytochemical containing formulationscarefully assay the alkaloid content of theextracts and formulations. Thechromatogram above illustrates baselineresolution of Cat’s Claw bark extractscontaining isopteropodine, pteropodine,mitraphylline, isomitraphylline,ryncophylline and isorynchophylline.
Alkaloids from Cat’s Clawanalyzed on a HAISIL 100 C18 column
P/N HS-2546-M185
HAISIL 100 - the Separations WorkHorse The 100Å pore size, monomeric bondedphase, and very high performance makeHAISIL 100 an ideal choice for separa-tions ranging from small molecules suchas carbamate pesticides and pharmaceu-
tical drugs to synthetic oligonucleotidesand small peptides. High efficiency,symmetrical peaks, and long column life ischaracteristic not only of the C8 andC18 reverse phase columns, but thenormal phase silica columns as well.
Carbamate Pesticide Analysis250 x 4.6mm HAISIL 100 C18
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HA I S I L 1 0 0 APPL ICAT IONS
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HA I S I L 3 0 0
HAISIL 300 For BiomoleculesPhases C18
Particle Sizes 5 and 10µm*Pore Size 300Å
Pore Volume 0.7mL/gmSurface Area 80m2/gm
%Carbon (w/w) C18 = 6%Phase type Monofunctional and fully endcappedSilica Class Type A
ApplicationsHAISIL 300 columns and cartridges areideal for biomolecule separations such aspeptides and small proteins. Optimizedphase bonding and column packingtechniques assure unsurpassed perform-ance and column-to-columnreproducibility. Short cartridge columnsare economical and are ideally suited forLC/MS applications. The column andcartridge offering ranges from 150µm to20mm I.D. which means that biosepara-tions can be optimized by selecting aformat that best fits your analyticalrequirements.
Micro-Preparative HPLC Higgins Analytical Cartridge Columns arehelping researchers understand theimmune response of living cells. An inves-tigation to understand the genetic con-trol of the immune response systems inorganisms involves the extraction, isola-tion, and identification of more than10,000 peptides. These distinct peptidesare bound to a major histocompatibilitycomplex (MHC) molecule on tumorcells.
Extraction of approximately 5x1010
cellsyield several nanomoles of a very com-plex peptide mixture. Several rounds ofRP-HPLC with different organic modifierson a 40x2.1mm PEEK cartridge column(HAISIL 300 C18 5µ m, P/N: HK-0421-W185) are performed to fractionate themixture into individual components.
The chromatograms on the left illustratehow this technique is being used to nar-row the search for the one peptide thatmarks a particular tumor cell as beingdiseased enabling it to be recognized bythe immune cells.
Fractionation of a Purified Tumor Cell Extract
Peptides ranging from 100 fmol to 1pmol are collected in 40secintervals during a 70min gradient from 0 - 60% MeCN with HFBAmodifier. A 40x2.1mm HAISIL 300 C18 5µm cartridge column(HK-0421-W185) is used.
Reanalysis of a Fraction from the Chromatogram above
The same 40x2.1mm HAISIL 300 cartridge is used for a 70mingradient from 0-60% MeCN with TFA modifier. Tic marks indicate40sec fraction collections. Peek and Titanium cartridge columns
used in the application described above.P/N: HK-0421-W185
Guide to HAISIL 300 Part NumbersHx-xxxx-W185 HAISIL 300 C18 5µmSee Page 23 for complete Part Number information
* 10µm is offered in HAISpeed 20 Guard Cartridges only. See pages 68-69.
Fast Analysis of a Complex DrugDelivery SystemMost pharmaceutical QC laboratoriesare faced with rapidly increasingdemands for high throughput capability.A good example of one laboratory's
approach to addressing a large numberof samples for analysis on a daily basis issummarized below. This novel drugdelivery system contains a small proteinand other components that span anorder of magnitude in molecular weight.All the compounds of interest wereeasily resolved in a single analysis with afast linear gradient on a 5cm longHAISIL 300 C18 5µm column.
Very High Peak CapacityDepending upon the type of informationa protein chemist is seeking, the HPLCanalysis of a complex digest can take 1to 2 hours. The following chromatogram
illustrates a high resolution tryptic digestmap of BSA on a 250x2.1mm HAISIL300 C18 5µm column indicates peakcapacities in excess of 100. Smallproteins, on the other hand can yieldfewer fragments, and if only a "finger-print" of a digest is required, high peakcapacity columns can be a waste oftime.
HA I S I L 3 0 0 APPL ICAT IONS
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Tryptic Map of BSAon HAISIL 300 C18 5µm 250x4.6mm
P/N HS-2546-W185
Peptide Map in 5 minutesThe figure below illustrates a very fast
peptide map of cytochrome-C. Theinformation that can be gleaned from thisfive minute profile is more than adequatefor many analytical requirements. Therobust 50x4.6mm HAISIL 300 C18column provides hundreds of runswithout performance loss even underthe aggressive 6ml/min flow conditionsused in this example.
The cytochrome-C is a good example ofpushing a column beyond its optimumoperating conditions (more than sixtimes optimum linear velocity) and stillbeing able to get desired results. Thesame column can be run under moreconventional conditions with a flattergradient to optain even higher resolutionseparations. The rhGH tryptic map belowwas done on a 5cm long column thatreplaced the conventional 25cm columnthat was used initially.
The Role of Column Length inGradient Separations Changes in column length have a directand linear effect on theoretical plates andanalysis time in isocratic separations.Column length plays a less important role,however, in gradient techniques. The threechromatograms below illustrate how wellpacked short HPLC columns yield verysimilar peptide separations under identicalgradient conditions.
For this study, the 2.1mm I.D. HAISIL 300C18 5µm columns ranged from 250 to100mm in length. While solvent andanalysis time savings may be minimal,column cost savings can be significantwhen using as short a column as possible.Short columns also exhibit lower pressureat high flow rates.
250x2.1mmHAISIL 300 C18 5µmHS-2521-W185
150x2.1mmHAISIL 300 C18 5µmHS-1521-W185
100x2.1mmHAISIL 300 C18 5µmHS-1021-W185
1. Gly-Tyr2. Val-Tyr-Val3. Met Enkephalin4. Leu Enkephalin5. Angiotensin II
Fast Tryptic Map of rhGH50x4.6mm HAISIL 300 C18
HA I S I L HL
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HAISIL HL High LoadPhases C18
Particle Sizes 5µmPore Size 100Å
Pore Volume 0.8mL/gmSurface Area 300m2/gm
%Carbon (w/w) C18 = 18%Phase type Monofunctional & fully endcappedSilica Class Type B
ApplicationsHAISIL HL “high load” columns and car-tridges are intended for applicationsrequiring maximum loading and selectivi-ty. The high surface area permits highbonded phase loading yet the monofunc-tional bonded phase and the absence ofmicro-pores eliminates the problems thatplague many of the so-called “high capac-ity” columns currently on the market.The Type B silica backbone is pure,robust, and stable under harsh condi-tions. Users report satisfactory lifetimewhen used for prolong periods at pH ashigh as 9.
High Speed Analysis of ParabenFood PreservativesParabens represent another example offood additives that are routinely analyzedby HPLC. Often, the food matrix beingassayed for preservative content requiresexhaustive cleanup and pre-filtration inorder to prevent clogging of columnswith nonporous or 2µm sorbentsrecently suggested for this analysis. As inthe previous diet cola example, thecompounds of interest are base lineresolved under isocratic conditions. Theinexpensive HAISIL HL 5µm 20x2.1mmcartridge column and the 50 secondanalysis time contribute significantlytowards reducing operating costs.
Fast Paraben Analysis
HAISIL HL C18 5µm20x2.1mm CartridgeLK-0221-C185
700µL/min, 40% MeCN/water
Abstract Previously, we presented a complete 20-minute "fast" Edman chemistry cycle forthe Applied Biosystems ProteinSequencer, HT series (49X-HT). Herewe describe a new "fast" cycle for thehigh sensitivity cLC series (49X-cLC)instrument. The 35-min cycle includes a20-min reversed phase capillary HPLCseparation of the phenylthiohydantoin(PTH)-amino acids. The normal cycletime of the 49x-cLC was approx. 50minutes; therefore approximately 10hours were required to complete 10residues of sequencing. With the newcycle, 10 residues of sequencing can be
completed in only 7.5 hours. Using thisrapid cycle on the 492 or 4-cLC proteinsequencer, 10 residues of sequencing on2 different proteins can be automaticallycompleted within a night.In developing this rapid cycle, we havecreated new cartridge and flask cyclesand sequencing methods. We are using ashorter C18 RP-HPLC capillary columnfor the chromatography and have devel-oped a new gradient. We will describeall the changes made to the standardsystem. We will also present protein andpeptide sequencing results that weregenerated using this rapid cycle.
A new “fast” cycle for high sensitivity Edman Sequencing
Poster 4P-52378th Annual Meeting of he Japanese Biochemical Society - Oct 2005Y. Shionoya, D. Dupont, H. Hiranuma, S. Ando
Guide to HAISIL HL Part NumbersLx-xxxx-C185 HAISIL HL C18 5µmSee Page 23 for complete Part Number information
HPLC Peptide Standards for RP onthe HAISIL HL C18 ColumnThe aromatic hydrocarbons that mostHPLC column manufacturers use toquality control columns render littleinformation about a column's selectivity
and performance characteristics forpeptides. The Canadian corporation, SPI,produces a well characterized yetchallenging peptide mixture that is veryuseful for quantifying HPLC columnperformance for peptides. The difficult toseparate first pair of peptides areresolved to near base line on HAISIL HLC18 columns. Many so-called peptideanalysis columns are unable to separatethis pair.
HA I S I L HL APPL ICAT IONS
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Speed and Selectivity for BallisticGradients on Complex LibrariesThe relative importance of particle sizeand selectivity are graphically illustratedbelow by the high speed analysis of a sixcomponent combinatorial library on aHAISIL HL C18 5µm column and twocompetitor’s C18 2µm columns. HAISILHL with its high carbon loading andsurface area of often works much betterthan expensive small particle sizecolumns that are prone to plugging upmore quickly.
Competitor “A”2µm C18
HAISIL HL5µm C18
Competitor “B”2µm C18
Water Soluble Vitamins
HAISIL HL C18 5µm 250x4.6mmA: 0.05mM KOAc, pH5; B: MeCNGradient: 2.5% - 30%B in 25min
Food AdditivesBeverage additive analysis is not only animportant industrial application, but italso serves as a practical and relativelysimple procedure for introductory HPLCcourses. Industry is increasingly driven byrapid analysis requirements and an HPLCcolumn can represent an overwhelmingexpense in a teaching environment. Thediet cola analysis in the chromatogram tothe left illustrates an extremely fastanalysis on an extremely inexpensiveHPLC column. A package containing five
of the 20x2.1mm cartridge column usedfor this analysis costs about half as muchas one moderately priced HPLC column.And, there's no need to pre-filter thecola sample through a 0.5µm filter assome column manufacturers recommendwhen using their expensive columnspacked with 2µm particles for thisanalysis! The readily available and non-toxic nature of the sample and the lowcost of Higgins Analytical cartridges hasmade the diet cola analysis an idealchoice for classroom purposes.
Basic Compounds in Combinatorial Mixtures
HAISIL HL C18P/N LS-1546-C185
Reverse Phase Chromatography ofBasic Compounds in CombinatorialMixturesIn the example at the top of the nextcolumn, A and B are single compoundscontaining two identical building blocks.A-B and B-A represent positionalisomers, each containing two buildingblocks.
This analysis was conducted on the crudeliquid-phase reaction mixture. Eachcomponent contains multiple secondary
and tertiary amine functionality yet thecombinatorial products can be rapidlychromatographed and exhibit no tailingon a 150x4.6mm HAISIL HL C18column using a simple linear 5% to 100%MeCN/water 0.1% TFA gradient.
Water Soluble Vitamin Analysis onHAISIL HL
1. H2N-RGAGGLGLGK-amide2. AC-RGGGGLGLGK-amide3. AC-RGAGGLGLGK-amide4. AC-RGVGGLGLGK-amide5. AC-RGVVGLGLGK-amide
“Hodges Test Peptide Mix”(from Synthetic Peptides, Inc.)
HAISIL HL C18 5µm 150x4.6mm P/N LS-1546-C185
Fast Diet Cola Analysis
HAISIL HL C18 5µm 2cm x 2.1mm Cartridge PN LK-0221-C185
15% MeCN/Water 200µL/min
Caffeine
Aspartame
Sodium Benzoate
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HEAVY
HEAVY Very Hydrophobic LoadingPhases C18
Particle Sizes 4.5µmPore Size 100Å
Pore Volume 1.1mL/gmSurface Area 450m2/gm
%Carbon (w/w) C18 = 22%Phase type Monofunctional & fully endcappedSilica Class Type B
ApplicationsKeeping in mind that relative retention(α) is a much more powerful term thanplates (N) in the expression for resolu-tion in chromatography, choosing a col-umn with different selectivity is almostalways more helpful than just going to asmaller particle size when trying toimprove a difficult separation. Our newHEAVY columns provide the highestretention of any column we manufacture.
Relative hydrophobicity of Higgins Analytical’s bonded phase sorbents.Contitions: 70% MeCN/Water
Guide to HEAVY Part NumbersVx-xxxx-C185 HEAVY C18 5µmSee Page 23 for complete Part Number information
Using a PICCOLO as a MicroboreGuard ColumnPICCOLO columns are normally used astrap columns but since they aremanufactured with the same techniques
we use to pack microbore HPLCcolumns, they are very efficient. Theirperformance makes them ideal for useas short microbore HPLC column or asa guard for placed in front of anotheranalytical microbore column. Thechromatograms in the figure belowillustrate typical performance when usedin these unconventional modes.
Why do HPLC columns plug up? A large percentage of HPLC columns diebecause the back pressure builds up to
levels that exceed the instrument’spractical limits. Most chromatographers(and many column manufacturers)believe this is because the inlet frit plugsup with particles. Actually, it is usually thepacked-bed that becomes plugged bysmaller than 1µm particles that passthrough in-line filters and the 2µm inletfrits. The SEM above illustrates therelative sizes of particles and pores.Higgins Analytical offers a wide variety ofguard cartridges that address thisproblem.
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INTERE ST ING COLUMN FACTS
The Role of Column Length inGradient Separations Changes in column length have a directand linear effect on theoretical platesand analysis time in isocratic separa-tions. Column length plays a lessimportant role, however, in gradienttechniques. The three chromatogramsbelow illustrate how well packed shortHPLC columns yield very similarpeptide separations under identicalgradient conditions.
For this study, the 2.1mm I.D. HAISIL300 C18 5µm columns ranged from250 to 100mm in length. While solventand analysis time savings may beminimal, column cost savings can besignificant when using as short a columnas possible. Short columns also exhibitlower pressure at high flow rates.
250x2.1mmHAISIL 300 C18 5µmHS-2521-W185
150x2.1mmHAISIL 300 C18 5µmHS-1521-W185
100x2.1mmHAISIL 300 C18 5µmHS-1021-W185
1. Gly-Tyr2. Val-Tyr-Val3. Met Enkephalin4. Leu Enkephalin5. Angiotensin II
5µm Silica Gel Particle
0.75µm Extra-Particle Flow Path
Piccolo Column performance as anAnalytical Column and as a Guard Column
Blue Trace: 10x1mm Targa C18 5µm Piccolo GuardColumn alone (P/N TP-0101-C185)
Red Trace: 50x1mm TARGA C18 5µm AnalyticalColumn (P/N TS-0501-C185)
Black Trace: 10x1mm Guard coupled to 50x1mmAnalytical Column
Peak #1: Dimethyl Phthalate Peak #2: Fluorene60% MeCN/water, 50µL/min
System Variance ~6µL2 at 50µL/min
SPRITE is Designed to Operate inEither Flow DirectionA SPRITE is made of PEEK and titaniumand can be connected directly to asample injection valve or directly to amass spectrometer’s source inlet.
PROTO™ 200 More Selectivity and Resolution for Peptides
Phases C4 and C18Particle Sizes 4.5 and 10µm
Pore Size 200ÅPore Volume 1.1mL/gmSurface Area 200m2/gm
%Carbon (w/w) C4. = 4.5%, C18= 14%Phase type Monofunctional & fully endcappedSilica Class Type B
14
PROTO200
Applications300Å wide pore HPLC columns aretraditionally used for biomolecule analysissince the molecular radii of largepeptides and proteins are bettermatched than with smaller pore sizecolumns (80 - 120Å) typically used forsmall molecule analysis. 300Å sorbents,however, have reduced surface area, thusless capacity and selectivity than wouldbe had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200presents a powerful alternative for theanalysis of peptides of ~19kD and less.
200
PROTO 200150x4.6mm
P/N/ RS-1546-D185
238EV150x4.6mm
218TP250x4.6mm
Phosphopeptide Purity AnalysisPeptide HPLC column performance,using the same gradient conditions, wasevaluated on the PROTO™ 200 C18, anew peptide analysis column obtainedfrom The Nest Group, Inc.. Against theindustry standard, Grace/Vydac® 218TPC18, and their newer peptide chemistry,Everest® C18, the data show that thePROTO™ 200 C18 column has superiorresolution to the 218TP chemistry andbetter resolution, especially for the morehydrophobic peptides, than the Everest®C18. In addition, the PROTO 200columns can be run without TFA, whichallows better detection when utilizingLC-MS.
Previously, the Everest chemistry hadbeen shown by Grace/Vydac to besuperior to all other wide pore columns,especially for the more hydrophobicpeptides. However, the ready availabilityof the PROTO™ 200 peptide columns,in either C4 or C18 chemistries, and incapillary through preparative sizes, nowallows researchers their first opportunityto save both time and money on a widervariety of applications, compared to theGrace/Vydac columns, and by extensionto all other peptide columns.
Comparison of Phosphopeptide Purity Analysis
Guide to PROTO 200 Part NumbersRx-xxxx-D045 PROTO 200 C4 5µmRx-xxxx-D185 PROTO 200 C18 5µmSee Page 23 for complete Part Number information
200
PROTO200
15
Vydac C18 238MS5115150 x 1.0mm
PROTO 200 C18150 x 1.0mm
Proto™ 200 C18 provides greater selectivity than the popular 300Å choice
Tryptic Digest of Carbamidomethylated BSA (16h, 20 pMols on column).
Column Size: 150 x 1.0mm
Column Brand:Upper Chromatogram: Vydac 300Å 238MS C18 5µm P/N 238MS5115Lower Chromatogram: Higgins 200Å Proto 200 C18 5µm P/N RS-1501-D185
Conditions:A = 0.1% formic acid containing 0.01% TFAB = 0.085% formic acid in CH3CN containing 0.01% TFAInitial 5 min isocratic hold at 5% B, then 60 min hyperbolic gradient to 65%BFlow 0.15 ml/min, Green & Black curves: Blank gradient profile
Higgins Analytical thanks Amos Heckendorf of The Nest Group (www.nestgrp.com) for these Proto 200 data.
200
16
PROTO 300
PROTO™300 Robust BioseparationsPhases C4 and C18
Particle Sizes 4.5 and 10µmPore Size 300Å
Pore Volume 0.9mL/gmSurface Area 100m2/gm
%Carbon (w/w) C4 = 3%, C18= 8%Phase type Monofunctional & fully endcappedSilica Class Type B
ApplicationsLow pH operating conditions and highpH column washing steps are typicalbioprocessing operating conditions thatcan severely limit the lifetime andperformance of widepore columnstypically used for protein and peptideanalysis. Higgins Analytical’s newPROTO300 has high capacity forenhanced resolution and is robustenough to withstand prolonged use atextreme pH (1.5 - 10).
Proto™300 C18
Manufacturer K300 C18
Manufacturer P300 C18
Stability Study Under ExtremelyAlkaline Conditions Experimental:150 x 4.6mm columns were studied. Allexperiments were conducted onduplicate columns. Solutes used forperformance measurement weredimethylphthalate and fluorene.
Step One:Column stability and measurementprecision was established for eachcolumn with multiple injections over18hrs under neutral conditions (70%MeCN/water, 1mL/min). All columnswere very stable. The chromatograms ofa column from each manufacturer at theend of the 18 hour period are shown onthe left hand side.
Step Two:Each column was flushed with 70%MeCN/0.5N NaOH solution at 1mL/minfor one hour (23ºC). The columns werethen purged and equilibrated under thestandard neutral conditions thenretested. The center chromatograms arethe results of the first test after neutralequilibration
Step Three:After the second step, the neutral testconditions were maintained for one hourand a repeat injection was made. Theresults are illustrated in thechromatograms on the right hand side ofthe figure.
While Higgins Analytical does notrecommend flushing any silica-basedHPLC column under such aggressiveconditions, these data show the superiorakaline stability of Proto™ 300 C18.
Guide to PROTO 300 Part NumbersRx-xxxx-W045 PROTO 300 C4 5µmRx-xxxx-W185 PROTO 300 C18 5µmSee Page 23 for complete Part Number information
PROTO 300
17
C8 Vydac®, 208TP5215, 5µm2.1 x 150mm
C4 PROTO™300, 5µm2.1 x 250mm
C8 Zorbax® Rapid Resolution 3.5µm2.1 x 150mm
Column: 250 x 2.1mm Higgins P/N RS-2521-W045 (5µm, 300Å)Vydac P/N 214TP52 (5µm, 300Å)
Mobile Phase: (A) 0.1%TFA in water, (B) 0.08%TFA in ACN, Flow Rate: 0.3minStart Conditions: 25%B, Linear Gradient Run Time 34min
Superior Resolution with PROTO™300 C4 for Protein Tryptic Digests
PROTO™ 300 C4 for Protein Analysis
Higgins Proto 300 C4250 x 2.1mm
Vydac 214TP52 C4250 x 2.1mm
Higgins Analytical thanks Amos Heckendorf of The Nest Group (www.nestgrp.com) for these data.
18
Phalanx is Higgins Analytical’s latest addi-tion to a family of unique high perform-ance HPLC sorbents. The novel end cap-ping technology of this new sorbent is acontinuation of the pioneering approachtaken in 1996 when we introducedTarga®, the first HPLC sorbentspecifically designed for efficientLC-MS applications without TFA.
Based on an ultrapure, Type-B silica back-bone, Phalanx has a relatively high sur-face area of 430 m2/gm, but its very tightpore size distribution eliminates theundesirable side effects of microporesthat often plague other high surfacematerials.
Optimum for Basic and Acidic com-pounds: Many modern HPLC sorbentsemploy embedded polar functionality.While these secondary structures canprovide unique selectivity and eliminateundesirable solute/sorbent interaction,they often compromise the useful rangeof solutes or buffers that can be used.The figure on the left and those on page2 illustrate that Phalanx columns pro-duce symmetrical peaks during the analy-sis of basic, neutral, and acidic com-pounds.
Wide pH operating range: The uniqueendcapping reduces acid/base hydrolysisof the bonded phase and greatly reducessilica solvation at high pH.
PHALANX
PHALANX Ultra High Density End CappingPhases C18
Particle Sizes 3, 4.5, 10µmPore Size 100Å
Pore Volume 1.1mL/gmSurface Area 430m2/gm
%Carbon (w/w) C18 = 18%Phase type Monofunctional & fully endcappedSilica Class Type B
ApplicationsPhalanx is uniquely suitable for theefficient analysis of both acidic and basicanalytes. In addition, its high capacity, goodstability at high pH, and fast equilibrationat extreme ranges of hydrophobicitymake Phalanx a powerful option forchallenging separation problems.
Phalanx HPLC columns and cartridges facilitate the development ofrobust assays for challenging analytes
Unique Features:• Ideal for Basic and Acidic Analytes• Wide pH Range Stability• High Capacity• No secondary Si-OH interaction• No metal contamination• Extreme hydrophobicity range
Standard Sizes:• Special products for LC-MS• 0.075 - 20mm I.D. range• Wide range of HPLC Cartridge and Column types• PEEK/titanium as well as stainless steel formats
High SelectivityWide range of solutes resolve quickly on a short HPLC columnPhalanx C18, 100x4.6mm, P/N: PS-1046-C185(1) Uracil, (2) Caffeine, (3) Phenol, (4) n-Butylbenzene, (5) o-Terphenyl, (6) n-Amylbenzene, (7) Triphenylene80% MeOH/water, 1mL/min
Guide to Phalanx Part NumberPx-xxxx-C185 Phalanx C18 5µmSee Page 23 for complete Part Number information
1
2
3
45
6
7
NEW
3 & 10
µm
19
Good Peak Symmetry for BasicCompounds
The very low solute/sorbent interactionof Phalanx C18 is clearly illustrated bythe analysis of basic compounds on ashort 5cm cartridge column. Phalanx C18, 50x2.1mm, P/N: PK-0521-C185(1) Propanediol, (2) Diphenyldramine,(3) Acenapthene, (4) Amitiptyline.65% MeOH/water 20mM KH2PO4 (pH 7.0), 200µL/min
Tricyclic Antidepressants
The very low solute/sorbent interactionof Phalanx C18 is clearly illustrated bythe analysis of basic compounds on a100x4.6mm HPLC column. Phalanx C18, 100x4.6mm, P/N: PS-1046-C185(1) Desipramine, (2) Nortriptyline, (3) Acenaphthene,(4) Imipramine, (5) Amitriptyline, (6) Trimipramine.70% MeOH/water 20mM KH2PO4 (pH 7.0), 1.0mL/min
Unbuffered Pyridine Test for Free Silanol Activity
A very sensitive test for residual silanolsproduces a symmetrical pyridine peak ina low organic mobile phase withoutbuffers. Phalanx C18, 50x2.1mm, P/N: PK-0521-C185(1) Uracil, (2) Phenol, (3) Pyridine40% MeCN/water, 200µL/min
NIST Test Mix for Metal Activity andResidual Silanols
Quininzarin, a strong metal chelator, andamitriptyline, a very sensitive solute forresidual silanols, both elute with goodrecovery and symmetrical peaks on bothTarga and Phalanx C18 columns. Targa® C18, 150x4.6mm, P/N: TS-1546-C185Phalanx™ C18, 150x4.6mm, P/N: PS-1546-C185(1) Uracil, (2) Toluene, (3) Ethylbenzene,(4) Quinizarin,(5) Amitiptyline.80% MeOH/buffer, 1.5mL/min
Nucleosides
Like its cousin Targa, Phalanx’s uniqueend-capping chemistry works equallywell at extreme hydrophilic andhydrophobic ranges. Phalanx C18, 50x2.1mm, P/N: PK-0521-C185(1) Cytosine, (2) Uracil, (3) Cytidine, (4) Uridine, (5) Thymine.100% Water, 1.0mL/min
TARGA® C18
PHALANX™ C18
1
2
3
4
1 2 3
4
5
1 2 3
4
5
1
2
3
45
1
2
3
45
6
1
2
3
PHALANX APPL ICAT IONS
See page 28 for an interestingcomparison of PHALANX toKROMASIL C18 for an analysis of apharmaceutical drug.
20
While TARGA columns give exceptionalperformance without TFA buffer, it doesnot mean that you are precluded fromusing it. In fact, the changes in selectivity
that comes with changing buffer acidscan be a powerful tool for separationoptimization. Notice how the peptideelution orders change between theacetic acid (above) and TFA (below)buffered chromatogram. This is due tothe diferences in pH of the acetic acidand TFA mobile phase.
TARGA
TARGA A Uniquely Shielded Surface Phases C8 and C18
Particle Sizes 3, 4.5 and 10µmPore Size 120Å
Pore Volume 0.8mL/gmSurface Area 330m2/gm
Carbon%(w/w) C18 = 18%Phase type Monofuctional with a unique polar and
bulky end-cappingSilica Class Type B
ApplicationsTARGA columns and cartridges areideal for applications requiring anabsence of secondary solute-silanol inter-action. Basic and acidic drugs and pep-tides can be chromatographed efficientlywith little or no buffer. Targa columns areparticularly well suited for LC/MS appli-cations where acetic acid or formic acidcan be substituted for TFA resulting insignificant increase in signal. While TFA isnot required, it does not preclude its useon TARGA columns. TARGA exhibitsextremely fast equilibration kinetics fromhigh to low organic phase compositions.It works well in 100% aqueous condi-tions for hydrophilic applications also.Peptide Mapping without TFA
The following chromatograms illustratethe differences in selectivity for peptidemaps of cytochrome-C when using0.05% TFA or 0.05% acetic acid buffer in
1%/min acetonitrile gradient on a short50x1.0mm TARGA C18 column. Bothdilute buffer examples are characterizedby sharp peaks and no tailing.
LC-MS without TFAResearchers have shown that HigginsAnalytical’s TARGA C8 and C18 phaseshave an extremely well protected silanolsurface. An acetonitrile/water 1%/mingradients with as little as 0.05% aceticacid gives a very satisfactory peptidemap on a short 5cm x 1mm TARGAC18 column. The ability to buffer withacetic or formic acid rather than TFAgreatly enhances signals for LC/MS tech-niques.
Basic Peptides
with 0.1% Acetic Acid pH 3.1TARGA C18 5µm 50x1.0mm
Basic Peptides
with 0.1% TFA pH 2.2TARGA C18 5µm 50x1.0mm
Tryptic Map of Human Ocular Lens Proteins beta A1 crystallin and gamma S crystallin
TARGA C18 5µm 150x0.5mmP/N TS-15M5-C185
Solvent A: 0.1% Acetic AcidSolvent B: 0.1% Acetic Acid, 65% MeCN0 - 50% B in 60min, 50 - 100%B in 15min
Sample is a tryptic digest of a 2D electrophoresis separat-ed spot digested from PDVF membrane. The two ocularproteins are ~25kD. The LC/MS zoom scan below con-firms the peak at 39.15min is singly charged, and softwareinterpretation of the MS/MS scan at the bottom confirmsthe 1156.6 ion as residues 84-94 in human gamma S crys-tallin.
LC/MS Scan
LC/MS/MS Scan
Guide to TARGA Part NumbersTx-xxxx-C183 Targa C18 3µmTx-xxxx-C185 Targa C18 5µmSee Page 23 for complete Part Number information
21
Unrivaled End Capping TechnologyEfforts to eliminate solute-silanol Targa HPLC columns are true to theLatin root of their name. TARGA,meaning “shield,” is precisely the effectthe unique bonded phase has on thesilica’s silanol surface.
Many HPLC column manufacturersillustrate the performance of theircolumns packed with inert, base deacti-vated, “Type B silica” bonded phases bythe chromatographic behavior of polarcompounds such as pyridine.Performance claims can be misleading,however, when mobile phases arebuffered to low pH or when theycontain high percentages of organicsolvent.
Favorable Kinetics at Aqueous orOrganic Mobile Phase ExtremesThe bulky moiety used for end cappingTARGA C8 and C18 sorbents presentother valuable selectivity characteristics inaddition to the extreme inertness tobasic and acidic compounds. Long chainalkane bonded phases collapse underlow organic phase conditions on mostreverse phase HPLC columns causingpoor reproducibility and slow reequilibra-tion. Fast gradients can be run onTARGA columns from initial 100%aqueous conditions with fast cycle timesand very good run-to-run reproducibility.TARGA columns can also be run isocrat-ically in 100% aqueous conditions forcarbohydrate analysis, or with only a fewpercent organic modifier to achieveselectivity on solutes that are retainedunder typical reverse phase conditions.
TARGA APPL ICAT IONS
Trace Catecholamine AnalysisIn vivo microdialysis present a challengeto both instrumentation and columndesign, especially when depended uponas a tool for routine analysis. Thechromatogram below illustrates rathypothalamus microdialysis analysis for5HT. Researchers report that the100x2.1mm Higgins Analytical PEEK andtitanium TARGA C18 column operatesroutinely at 0.2 - 100 nM detectionlevels.
5HT Analysis of Rat Hypothalamus Dialysate
TARGA C18 5µm 100x2.1mmP/N TS-1021-C185
NaH2PO4 0.15M, OSA 0.74mM, EDTA 0.1mMpH 2.87 (orthophosphoric acid) 2% IPA
Instrumentation: Flux Rheos Isocratic Pump, In-line SolventDegasser, Spark Holland Triathlon Autosampler, AntecINTRO EC Detector
Detection levels of 0.2 - 100 nM are routinely achieved.
TARGA Compared to a Competitor’s Hydophilic Column
150x4.6mm, 20% MeCN/water (unbuffered) 1mL/min
TARGA’s Superior HydrophilicReverse Phase PropertiesSeveral manufacturers offer reversephase column products with imbeddedpolar functionality intended to enhancethe chromatography of hydrophiliccompounds like those illustrated in theprevious antibacterial example.Unfortunately, most of these approachesintroduce undesirable secondary effectsthat preclude their use with acidic orbasic solutes unlike TARGA with itsunique bonded phase.
Fat-Soluble Vitamin Assay
TARGA C18 5µm 100x2.1mmPart Number TS-1021-C185
3% water in MeOH mobile phase200µl/min, diode array detection
Analysis of Very Polar Antibacterials with a Highly Aqueous Mobile Phase
1. Vancomycin, 2. Cefotaxime, 3. Cefazolin
TARGA C18 100x2.1mm TS-1021-C185Mobile Phase: 92% Water with 0.l% TFA pH2.5,
8% MeOH, DAD at 230nm
Unbuffered 20% MeCN/water
Anti-Hypertensives
TARGA C18 5µm 150x4.6mmPart Number TS-1546-C185
25% MeCN/20mM NaH2PO4 pH3.2 1mL/min 260nm
1. Triamterene 2. Hydrochlorothiazide
Analgesics and Anti-Inflammatorys
TARGA C18 5µm 150x4.6mmPart Number TS-1546-C185
45% MeCN/20mM NaH2PO4 pH3.2 1mL/min 260nm
1. Acetaminophen 2. Indoprofen3. Naproxan 4. Indomethacin5. Ibuprofen
A Popular Hydrophilic RP Column
TARGA C18 Column
Pyridine
Pyridine Phenol
Phenol
We Manufacture Products with Internal Diameters Ranging Almost Three Orders of MagnitudeThis catalog contains over one thousand products manufactured with over fifteen different phases in an unequaled variety of for-mats. This generous offering makes it easy to choose the proper column for your application.
The columns and cartridges included in this version of the Higgins Analytical cat-alog have internal diameters ranging from 75µm to 20mm. Not listed are our30mm and 50mm i.d. prep-columns. The bulk of our business in these large for-mats is based on Proto 200 and Proto 300 C4 and C18 products on particlesizes ranging from 10µm to 50µm geared for biomolecule purification - especiallysynthetic peptides.
As outlined in the paragraph below, another service we provide is custom manu-facturing for columns of all sizes, 30mm and 50mm prep-columns included, withsorbents produced by manufacturers other than ourselves. So, if you are interest-ed in one of our many sorbents, or another material that you may prefer, pleasegive us a call to discuss the details.
UNEQUALED FLEXIBILITY
22
S P EC IALTY COLUMNS AND S ERV ICE S
PTC Amino Acid AnalysisHiggins Analytical now manufacturers the PTC Amino Acid analysis column used on the Applied Biosystems Model 420 Analyzer.We have approval from AB to manufacture these columns and we can provide a certificate indicating that our columns have theidentical form and function as the product previously supplied by AB.
Part Number: 0711-0204 Price: $600 Always available from stock.
A recent publication cites a Higgins Analytical PTC column: Mass-spectrometric study of changes in allergen proteins by application ofcontrolled chemical reaction, where attempts to identify stable peptide markers from hazelnut and soybean were made.
C. Platteau, T. Cucu, R. Dobson, S. Fourdrilis, C. Bourgeon, D. Ebo, M. De Loose, I.Taverniers, E. Daeseleire, B. De Meulenaer, B. Devreese, E. DePauw, G. Maghuin-Rogister, M.-L. Scippo, W. Stevens, C. Bridts. “Development of an integrated strategy for controlling the allergen issue in theBelgian food and catering industry ALLERRISK”. Final Report. Brussels : Belgian Science Policy 2011 – 109 p. (Research Programme Sciencefor a Sustainable Development), Page 54
http://www.belspo.be/belspo/SSD/science/Reports/ALLERRISK_FinRep%20ML.pdf
Custom Manufacturing ServiceBesides being known worldwide for our huge variety of high quality and uncompromisingly affordable Higgins Analytical products,custom labeled manufacturing represents a sizeable part of our business. In fact, a lot of people might be very surprised if theylearned that their favorite competitive column was actually packed by Higgins Analytical. In addition to being based on our ownunique sorbents, many of the products we manufacture are produced with our customers’ own proprietary media. So, if your com-pany feels it might want to add a selection of high quality HPLC columns to its product offering, or if you need large numbers of aparticular column or device for your internal analytical needs, please give us a call. Higgins Analytical is very expert at providing thistype of service.
If you can put it in a bottle, Higgins Analytical will pack it into a column. If we’re unsuccessful, it won’t cost a thing!
23
GU IDE TO H IGG INS ANALYT ICAL’ S PART NUMBER ING SYSTEM
HPLC Columns and CartridgesHiggins Analytical HPLC column productpart numbers are based on a 2-4-4character pattern. For example:
TS-1521-C185
is the part number for a conventional15cm x 2.1mm stainless steel columnpacked with Targa C18 5µm sorbent.
The two character prefix indicates sorbent and hardware type.
Prefix Character 1 (Sorbent)X-XXXX-XXXX
C = CLIPEUSH = HAISIL 100 or HAISIL 300L = HAISIL HLP = PHALANXR = PROTO 200 or 300T = TARGAV = HEAVY
Prefix Character 2 (Hardware)X-XXXX-XXXX
FS = Capellini 6-32 columnPS = Capellini 10-32 columnS = Stainless steel HPLC ColumnP = PEEK HPLC columnF = Stainless steel cartridge columnK = PEEK cartridge columnR = Sprite Direct-Connect column
Note: Microbore columns have the sec-ond character, S, like stainless steelcolumns, but they are glass lined.
The four center characters indicate thecolumn or cartridge length (cm) and internal diameter (mm).
Center Characters 1 and 2 (Length)XX-XX-XXX
03 = 3cm04 = 4cm05 = 5cm07 = 7.5cm10 = 10cm12 = 12.5cm15 = 15cm20 = 20cm22 = 22cm25 = 25cm30 = 30cm50 = 50cm
The four center characters indicate the column or cartridge length (cm) and internal diameter (mm).
Center Characters 3 and 4 (I.D.)XX-XX-XXXX
07 = 0.075mmM1 = 0.1mm15 = 0.15mmM3 = 0.3mmM5 = 0.5mmM8 = 0.8mm01 = 1.0mm21 = 2.1mm30 = 3.0mm32 = 3.2mm39 = 3.9mm40 = 4.0mm46 = 4.6mm10 = 10mm20 = 20mm
The four character suffix indicates sorbentchemistry and particle size.
Suffix Characters 1 to 3 (Phase)XX-XXXX-X
C04 = Butyl or C4C08 = Octyl or C8C18 = ODS or C18CNP = Cyano or NitrileD04 = 200Å C4D18 = 200Å C18M08 = HAISIL 100 C8M18 = HAISIL 100 C18NH2 = AminoPHN = PhenylSIL = Silica Gel W04 = 300Å C4W08 = 300Å C8W18 = 300Å C18
Suffix Character 4 (Particle Size)XX-XXXX-XXX
1 = 10µm2 = 10 - 15µm3 = 3 or 3.5µm5 = 4 - 5µm7 = 15 - 20µm
Notes: (1) Standard configurations are highlighted.(2) Polymeric phases have a 3-4-6 pattern.
The first two prefix charactes are PL.The last character denotes particle size.
PLx-xxxx-xxxxxx
Cartridge HoldersGuardsTwo types of holders for 2cm long PEEKand s.s. cartridges are offered.
HK-GUARD-FF Female/FemaleHK-GUARD-FM Direct Connect
Analytical Cartridge HoldersThree internal diameters (2.1, 3.2, and4.6mm) and three lengths are offered.
HK-HOLD-40 for 4cm long cartridgeHK-HOLD-50 for 5cm long cartridgeHK-HOLD-100 for 10cm long cartridge
HAISpeed 10 Cartridge HoldersThese 10mm i.d. cartridges are offered inthree lengths.
10-HOLD-30 for 3cm long cartridge10-HOLD-50 for 5cm long cartridge10-HOLD-100 for 10cm long cartridge
HAISpeed 20 Cartridge Holder20mm i.d. prep guard cartridges require a25mm long cartridge holder.
20-HOLD-25 for 20 x 25mm cartridge
24
HPLC ColumnsWide Range of Stainless Steel HPLC COLUMNS
Widest Range of Hardware Typesand Sizes Available AnywhereHiggins Analytical manufacturers thewidest range of column formats in thewidest range of hardware types availableanywhere. Conventional HPLC columnsare offered in stainless steel hardware.Frit materials include stainless steel,Carpenter C, titanium. Standard lengthsrange from 3.3cm to 25 cm. Customcolumns are routinely manufactured inodd length ranging from 3.3 to 50cm.The internal diameters of our entireHPLC column and cartridge offeringrange from 75µm to 20mm, over twoorders of magnitude.
Guide to Some Standard HPLC Column Part NumbersxS-xxxx-xxxx Stainless Steel (ss) columnxP-xxxx-xxxx PEEK columnxS-03xx-xxxx 3.5cm ss column Shortest standard sizexS-25xx-xxxx 25cm ss column Longest standard sizexP-05xx-xxxx 5cm PEEK column Shortest standard sizexP-25xx-xxxx 25cm PEEK column Longest standard sizexS-xx21-xxxx 2.1mm ss I.D. Smallest standard I.D.xP-xx21-xxxx 2.1mm PEEK I.D. Smallest standard I.D.xS-xx46-xxxx 4.6mm ss I.D. Popular standard I.D.xP-xx46-xxxx 4.6mm PEEK I.D. Popular standard I.D.See Page 23 for complete Part Number information
CLIPEUS Analytical Columns - Stainless SteelCLIPEUS C8 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-C085 $270 CS-0546-C085 $280 CS-1046-C085 $290 CS-1546-C085 $325 CS-2546-C085 $35030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmCS-0340-C085 $270 CS-0540-C085 $280 CS-1040-C085 $290 CS-1540-C085 $325 CS-2540-C085 $35030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmCS-0330-C085 $270 CS-0530-C085 $280 CS-1030-C085 $290 CS-1530-C085 $325 CS-2530-C085 $35030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmCS-0321-C085 $270 CS-0521-C085 $280 CS-1021-C085 $290 CS-1521-C085 $325 CS-2521-C085 $350
CLIPEUS C18 10µm 4.6mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-C181 $245 CS-0546-C181 $260 CS-1046-C181 $270 CS-1546-C181 $295 CS-2546-C181 $320
CLIPEUS C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-C185 $270 CS-0546-C185 $280 CS-1046-C185 $290 CS-1546-C185 $325 CS-2546-C185 $35030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmCS-0340-C185 $270 CS-0540-C185 $280 CS-1040-C185 $290 CS-1540-C185 $325 CS-2540-C185 $35030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmCS-0330-C185 $270 CS-0530-C185 $280 CS-1030-C185 $290 CS-1530-C185 $325 CS-2530-C185 $35030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmCS-0321-C185 $270 CS-0521-C185 $280 CS-1021-C185 $290 CS-1521-C185 $325 CS-2521-C185 $350
ANALYT ICAL COLUMN ORDER ING INFORMAT ION
25
CLIPEUS Analytical Columns - Stainless SteelCLIPEUS Cyano 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-CNP5 $280 CS-0546-CNP5 $290 CS-1046-CNP5 $300 CS-1546-CNP5 $345 CS-2546-CNP5 $36530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmCS-0340-CNP5 $280 CS-0540-CNP5 $290 CS-1040-CNP5 $300 CS-1540-CNP5 $345 CS-2540-CNP5 $36530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmCS-0330-CNP5 $280 CS-0530-CNP5 $290 CS-1030-CNP5 $300 CS-1530-CNP5 $345 CS-2530-CNP5 $36530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmCS-0321-CNP5 $280 CS-0521-CNP5 $290 CS-1021-CNP5 $300 CS-1521-CNP5 $345 CS-2521-CNP5 $365
CLIPEUS Phenyl 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-PHN5 $280 CS-0546-PHN5 $290 CS-1046-PHN5 $300 CS-1546-PHN5 $345 CS-2546-PHN5 $36530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmCS-0340-PHN5 $280 CS-0540-PHN5 $290 CS-1040-PHN5 $300 CS-1540-PHN5 $345 CS-2540-PHN5 $36530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmCS-0330-PHN5 $280 CS-0530-PHN5 $290 CS-1030-PHN5 $300 CS-1530-PHN5 $345 CS-2530-PHN5 $36530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmCS-0321-PHN5 $280 CS-0521-PHN5 $290 CS-1021-PHN5 $300 CS-1521-PHN5 $345 CS-2521-PHN5 $365
CLIPEUS Silica 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-SIL5 $245 CS-0546-SIL5 $250 CS-1046-SIL5 $260 CS-1546-SIL5 $295 CS-2546-SIL5 $31530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmCS-0340-SIL5 $245 CS-0540-SIL5 $250 CS-1040-SIL5 $260 CS-1540-SIL5 $295 CS-2540-SIL5 $31530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmCS-0330-SIL5 $245 CS-0530-SIL5 $250 CS-1030-SIL5 $260 CS-1530-SIL5 $295 CS-2530-SIL5 $31530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmCS-0321-SIL5 $245 CS-0521-SIL5 $250 CS-1021-SIL5 $260 CS-1521-SIL5 $295 CS-2521-SIL5 $315
HAISIL100 Analytical Columns - Stainless SteelHAISIL 100 C8 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmHS-0346-M085 $270 HS-0546-M085 $280 HS-1046-M085 $290 HS-1546-M085 $325 HS-2546-M085 $35030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmHS-0340-M085 $270 HS-0540-M085 $280 HS-1040-M085 $290 HS-1540-M085 $325 HS-2540-M085 $35030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmHS-0330-M085 $270 HS-0530-M085 $280 HS-1030-M085 $290 HS-1530-M085 $325 HS-2530-M085 $35030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmHS-0321-M085 $270 HS-0521-M085 $280 HS-1021-M085 $290 HS-1521-M085 $325 HS-2521-M085 $350
HAISIL 100 C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmHS-0346-M185 $270 HS-0546-M185 $280 HS-1046-M185 $290 HS-1546-M185 $325 HS-2546-M185 $35030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmHS-0340-M185 $270 HS-0540-M185 $280 HS-1040-M185 $290 HS-1540-M185 $325 HS-2540-M185 $35030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmHS-0330-M185 $270 HS-0530-M185 $280 HS-1030-M185 $290 HS-1530-M185 $325 HS-2530-M185 $35030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmHS-0321-M185 $270 HS-0521-M185 $280 HS-1021-M185 $290 HS-1521-M185 $325 HS-2521-M185 $350
ANALYT ICAL COLUMN ORDER ING INFORMAT ION
26
HAISIL100 Analytical Columns - Stainless Steel (cont.)HAISIL 100 Silica 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmHS-0346-SIL5 $245 HS-0546-SIL5 $250 HS-1046-SIL5 $260 HS-1546-SIL5 $295 HS-2546-SIL5 $31530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmHS-0340-SIL5 $245 HS-0540-SIL5 $250 HS-1040-SIL5 $260 HS-1540-SIL5 $295 HS-2540-SIL5 $31530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmHS-0330-SIL5 $245 HS-0530-SIL5 $250 HS-1030-SIL5 $260 HS-1530-SIL5 $295 HS-2530-SIL5 $31530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmHS-0321-SIL5 $245 HS-0521-SIL5 $250 HS-1021-SIL5 $260 HS-1521-SIL5 $295 HS-2521-SIL5 $315
HAISIL300 Analytical Columns - Stainless SteelHAISIL 300 C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmHS-0346-W185 $310 HS-0546-W185 $320 HS-1046-W185 $350 HS-1546-W185 $375 HS-2546-W185 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmHS-0340-W185 $310 HS-0540-W185 $320 HS-1040-W185 $350 HS-1540-W185 $375 HS-2540-W185 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmHS-0330-W185 $310 HS-0530-W185 $320 HS-1030-W185 $350 HS-1530-W185 $375 HS-2530-W185 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmHS-0321-W185 $310 HS-0521-W185 $320 HS-1021-W185 $350 HS-1521-W185 $375 HS-2521-W185 $405
HAISIL HL Analytical Columns - Stainless SteelHAISIL HL C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmLS-0346-C185 $380 LS-0546-C185 $390 LS-1046-C185 $400 LS-1546-C185 $425 LS-2546-C185 $44030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmLS-0340-C185 $380 LS-0540-C185 $390 LS-1040-C185 $400 LS-1540-C185 $425 LS-2540-C185 $44030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmLS-0330-C185 $380 LS-0530-C185 $390 LS-1030-C185 $400 LS-1530-C185 $425 LS-2530-C185 $44030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmLS-0321-C185 $380 LS-0521-C185 $390 LS-1021-C185 $400 LS-1521-C185 $425 LS-2521-C185 $440
HEAVY Analytical Columns - Stainless SteelHEAVY C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmVS-0346-C185 $310 VS-0546-C185 $320 VS-1046-C185 $350 VS-1546-C185 $375 VS-2546-C185 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmVS-0340-C185 $310 VS-0540-C185 $320 VS-1040-C185 $350 VS-1540-C185 $375 VS-2540-C185 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmVS-0330-C185 $310 VS-0530-C185 $320 VS-1030-C185 $350 VS-1530-C185 $375 VS-2530-C185 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmVS-0321-C185 $310 VS-0521-C185 $320 VS-1021-C185 $350 VS-1521-C185 $375 VS-2521-C185 $405
HPLC Columns (continued)Wide Range of Stainless Steel HPLC COLUMNS
ANALYT ICAL COLUMN ORDER ING INFORMAT ION
27
PROTO 200 Analytical Columns - Stainless SteelPROTO 200 C4 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmRS-0346-D045 $310 RS-0546-D045 $320 RS-1046-D045 $350 RS-1546-D045 $375 RS-2546-D045 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmRS-0340-D045 $310 RS-0540-D045 $320 RS-1040-D045 $350 RS-1540-D045 $375 RS-2540-D045 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmRS-0330-D045 $310 RS-0530-D045 $320 RS-1030-D045 $350 RS-1530-D045 $375 RS-2530-D045 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmRS-0321-D045 $310 RS-0521-D045 $320 RS-1021-D045 $350 RS-1521-D045 $375 RS-2521-D045 $405
PROTO 200 C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmRS-0346-D185 $310 RS-0546-D185 $320 RS-1046-D185 $350 RS-1546-D185 $375 RS-2546-D185 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmRS-0340-D185 $310 RS-0540-D185 $320 RS-1040-D185 $350 RS-1540-D185 $375 RS-2540-D185 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmRS-0330-D185 $310 RS-0530-D185 $320 RS-1030-D185 $350 RS-1530-D185 $375 RS-2530-D185 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmRS-0321-D185 $310 RS-0521-D185 $320 RS-1021-D185 $350 RS-1521-D185 $375 RS-2521-D185 $405
PROTO 300 Analytical Columns - Stainless SteelPROTO 300 C4 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmRS-0346-W045 $310 RS-0546-W045 $320 RS-1046-W045 $350 RS-1546-W045 $375 RS-2546-W045 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmRS-0340-W045 $310 RS-0540-W045 $320 RS-1040-W045 $350 RS-1540-W045 $375 RS-2540-W045 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmRS-0330-W045 $310 RS-0530-W045 $320 RS-1030-W045 $350 RS-1530-W045 $375 RS-2530-W045 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmRS-0321-W045 $310 RS-0521-W045 $320 RS-1021-W045 $350 RS-1521-W045 $375 RS-2521-W045 $405
PROTO 300 C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmRS-0346-W185 $310 RS-0546-W185 $320 RS-1046-W185 $350 RS-1546-W185 $375 RS-2546-W185 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmRS-0340-W185 $310 RS-0540-W185 $320 RS-1040-W185 $350 RS-1540-W185 $375 RS-2540-W185 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmRS-0330-W185 $310 RS-0530-W185 $320 RS-1030-W185 $350 RS-1530-W185 $375 RS-2530-W185 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmRS-0321-W185 $310 RS-0521-W185 $320 RS-1021-W185 $350 RS-1521-W185 $375 RS-2521-W185 $405
ANALYT ICAL COLUMN ORDER ING INFORMAT ION
200
28
PHALANX Analytical Columns - Stainless SteelPHALANX C18 3µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmPS-0346-C183 $380 PS-0546-C183 $390 PS-1046-C183 $440 PS-1546-C183 $475 PS-2546-C183 $50030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmPS-0340-C183 $380 PS-0540-C183 $390 PS-1040-C183 $440 PS-1540-C183 $475 PS-2540-C183 $50030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmPS-0330-C183 $380 PS-0530-C183 $390 PS-1030-C183 $440 PS-1530-C183 $475 PS-2530-C183 $50030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmPS-0321-C183 $380 PS-0521-C183 $390 PS-1021-C183 $440 PS-1521-C183 $475 PS-2521-C183 $500
PHALANX C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmPS-0346-C185 $310 PS-0546-C185 $320 PS-1046-C185 $350 PS-1546-C185 $375 PS-2546-C185 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmPS-0340-C185 $310 PS-0540-C185 $320 PS-1040-C185 $350 PS-1540-C185 $375 PS-2540-C185 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmPS-0330-C185 $310 PS-0530-C185 $320 PS-1030-C185 $350 PS-1530-C185 $375 PS-2530-C185 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmPS-0321-C185 $310 PS-0521-C185 $320 PS-1021-C185 $350 PS-1521-C185 $375 PS-2521-C185 $405
ANALYT ICAL COLUMN ORDER ING INFORMAT ION
NEW 3µm
HPLC Columns (continued)Wide Range of Stainless Steel HPLC COLUMNS
Comparison of PHALANX to Kromasil C18 for a Pharmaceutical Drug Analysis150 X 4.6mm Columns, 1mL/min Linear Gradient for 50 minutes
Higgins Analytical thanks Amos Heckendorf of The Nest Group (www.nestgrp.com) for these data.
PHALANX Full Scale Chromatogram KROMASIL C18 Full Scale Chromatogram
PHALANX Expanded Scale Chromatogram KROMASIL C18 Expanded Scale Chromatogram
ANALYT ICAL COLUMN ORDER ING INFORMAT ION
29
See pages 62 - 63 for information on the wide variety of Guard Cartridges available for these Analytical Columns
TARGA Analytical Columns - Stainless SteelTARGA C8 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns
30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmTS-0346-C085 $290 TS-0546-C085 $300 TS-1046-C085 $320 TS-1546-C085 $350 TS-2546-C085 $37030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmTS-0340-C085 $290 TS-0540-C085 $300 TS-1040-C085 $320 TS-1540-C085 $350 TS-2540-C085 $37030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmTS-0330-C085 $290 TS-0530-C085 $300 TS-1030-C085 $320 TS-1530-C085 $350 TS-2530-C085 $37030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmTS-0321-C085 $290 TS-0521-C085 $300 TS-1021-C085 $320 TS-1521-C085 $350 TS-2521-C085 $370
TARGA C18 3µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmTS-0346-C183 $365 TS-0546-C183 $375 TS-1046-C183 $400 TS-1546-C183 $430 TS-2546-C183 $45530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmTS-0340-C183 $365 TS-0540-C183 $375 TS-1040-C183 $400 TS-1540-C183 $430 TS-2540-C183 $45530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmTS-0330-C183 $365 TS-0530-C183 $375 TS-1030-C183 $400 TS-1530-C183 $430 TS-2530-C183 $45530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmTS-0321-C183 $365 TS-0521-C183 $375 TS-1021-C183 $400 TS-1521-C183 $430 TS-2521-C183 $455
TARGA C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmTS-0346-C185 $290 TS-0546-C185 $300 TS-1046-C185 $320 TS-1546-C185 $350 TS-2546-C185 $37030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmTS-0340-C185 $290 TS-0540-C185 $300 TS-1040-C185 $320 TS-1540-C185 $350 TS-2540-C185 $37030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmTS-0330-C185 $290 TS-0530-C185 $300 TS-1030-C185 $320 TS-1530-C185 $350 TS-2530-C185 $37030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmTS-0321-C185 $290 TS-0521-C185 $300 TS-1021-C185 $320 TS-1521-C185 $350 TS-2521-C185 $370
30
CLIPEUS Microbore ColumnsCLIPEUS C8 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmCS-0301-C085 $310 CS-0501-C085 $320 CS-1001-C085 $350 CS-1501-C085 $375 CS-2501-C085 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmCS-03M8-C085 $310 CS-05M8-C085 $320 CS-10M8-C085 $350 CS-15M8-C085 $375 CS-25M8-C085 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmCS-03M5-C085 $310 CS-05M5-C085 $320 CS-10M5-C085 $350 CS-15M5-C085 $375 CS-25M5-C085 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmCS-03M3-C085 $310 CS-05M3-C085 $320 CS-10M3-C085 $350 CS-15M3-C085 $375 CS-25M3-C085 $405
CLIPEUS C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmCS-0301-C185 $310 CS-0501-C185 $320 CS-1001-C185 $350 CS-1501-C185 $375 CS-2501-C185 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmCS-03M8-C185 $310 CS-05M8-C185 $320 CS-10M8-C185 $350 CS-15M8-C185 $375 CS-25M8-C185 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmCS-03M5-C185 $310 CS-05M5-C185 $320 CS-10M5-C185 $350 CS-15M5-C185 $375 CS-25M5-C185 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmCS-03M3-C185 $310 CS-05M3-C185 $320 CS-10M3-C185 $350 CS-15M3-C185 $375 CS-25M3-C185 $405
CLIPEUS Cyano 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmCS-0301-CNP5 $325 CS-0501-CNP5 $340 CS-1001-CNP5 $365 CS-1501-CNP5 $390 CS-2501-CNP5 $42530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmCS-03M8-CNP5 $325 CS-05M8-CNP5 $340 CS-10M8-CNP5 $365 CS-15M8-CNP5 $390 CS-25M8-CNP5 $42530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmCS-03M5-CNP5 $325 CS-05M5-CNP5 $340 CS-10M5-CNP5 $365 CS-15M5-CNP5 $390 CS-25M5-CNP5 $42530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmCS-03M3-CNP5 $325 CS-05M3-CNP5 $340 CS-10M3-CNP5 $365 CS-15M3-CNP5 $390 CS-25M3-CNP5 $425
CLIPEUS Phenyl 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmCS-0301-PHN5 $325 CS-0501-PHN5 $340 CS-1001-PHN5 $365 CS-1501-PHN5 $390 CS-2501-PHN5 $42530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmCS-03M8-PHN5 $325 CS-05M8-PHN5 $340 CS-10M8-PHN5 $365 CS-15M8-PHN5 $390 CS-25M8-PHN5 $42530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmCS-03M5-PHN5 $325 CS-05M5-PHN5 $340 CS-10M5-PHN5 $365 CS-15M5-PHN5 $390 CS-25M5-PHN5 $42530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmCS-03M3-PHN5 $325 CS-05M3-PHN5 $340 CS-10M3-PHN5 $365 CS-15M3-PHN5 $390 CS-25M3-PHN5 $425
MICROBORE HPLC COLUMN ORDER ING INFORMAT ION
Microbore HPLC ColumnsWide Range of 1mm, 0.8mm, 0.5mm, and 0.3mm Columns
Widest Range of Microbore columnsizes with a wide range of sorbentsHiggins Analytical manufacturers thewidest range of Microbore columnformats available anywhere. In addition tothe glass-lined columns ranging from300µm to 1mm I.D., we also porvideunique PEEK/Titanium microborecolumns for specialized applications (seePiccolo).
Guide to MICROBORE Part NumbersxS-xx01-xxxx 1.0mm MicroborexS-xxM8-xxxx 0.8mm MicroborexS-xxM5-xxxx 0.5mm MicroborexS-xxM3-xxxx 0.3mm MicroboreSee Page 23 for complete Part Number information
31
CLIPEUS Microbore Columns (cont.)CLIPEUS Silica 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmCS-0301-SIL5 $310 CS-0501-SIL5 $320 CS-1001-SIL5 $350 CS-1501-SIL5 $375 CS-2501-SIL5 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmCS-03M8-SIL5 $310 CS-05M8-SIL5 $320 CS-10M8-SIL5 $350 CS-15M8-SIL5 $375 CS-25M8-SIL5 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmCS-03M5-SIL5 $310 CS-05M5-SIL5 $320 CS-10M5-SIL5 $350 CS-15M5-SIL5 $375 CS-25M5-SIL5 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmCS-03M3-SIL5 $310 CS-05M3-SIL5 $320 CS-10M3-SIL5 $350 CS-15M3-SIL5 $375 CS-25M3-SIL5 $405
HAISIL100 Microbore ColumnsHAISIL 100 C8 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmHS-0301-M085 $310 HS-0501-M085 $320 HS-1001-M085 $350 HS-1501-M085 $375 HS-2501-M085 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmHS-03M8-M085 $310 HS-05M8-M085 $320 HS-10M8-M085 $350 HS-15M8-M085 $375 HS-25M8-M085 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmHS-03M5-M085 $310 HS-05M5-M085 $320 HS-10M5-M085 $350 HS-15M5-M085 $375 HS-25M5-M085 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmHS-03M3-M085 $310 HS-05M3-M085 $320 HS-10M3-M085 $350 HS-15M3-M085 $375 HS-25M3-M085 $405
HAISIL 100 C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmHS-0301-M185 $310 HS-0501-M185 $320 HS-1001-M185 $350 HS-1501-M185 $375 HS-2501-M185 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmHS-03M8-M185 $310 HS-05M8-M185 $320 HS-10M8-M185 $350 HS-15M8-M185 $375 HS-25M8-M185 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmHS-03M5-M185 $310 HS-05M5-M185 $320 HS-10M5-M185 $350 HS-15M5-M185 $375 HS-25M5-M185 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmHS-03M3-M185 $310 HS-05M3-M185 $320 HS-10M3-M185 $350 HS-15M3-M185 $375 HS-25M3-M185 $405
HAISIL 100 Silica 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmHS-0301-SIL5 $310 HS-0501-SIL5 $320 HS-1001-SIL5 $350 HS-1501-SIL5 $375 HS-2501-SIL5 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmHS-03M8-SIL5 $310 HS-05M8-SIL5 $320 HS-10M8-SIL5 $350 HS-15M8-SIL5 $375 HS-25M8-SIL5 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmHS-03M5-SIL5 $310 HS-05M5-SIL5 $320 HS-10M5-SIL5 $350 HS-15M5-SIL5 $375 HS-25M5-SIL5 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmHS-03M3-SIL5 $310 HS-05M3-SIL5 $320 HS-10M3-SIL5 $350 HS-15M3-SIL5 $375 HS-25M3-SIL5 $405
MICROBORE HPLC COLUMN ORDER ING INFORMAT ION
32
HAISIL300 Microbore ColumnsHAISIL 300 C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmHS-0301-W185 $320 HS-0501-W185 $330 HS-1001-W185 $350 HS-1501-W185 $375 HS-2501-W185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmHS-03M8-W185 $320 HS-05M8-W185 $330 HS-10M8-W185 $350 HS-15M8-W185 $375 HS-25M8-W185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmHS-03M5-W185 $320 HS-05M5-W185 $330 HS-10M5-W185 $350 HS-15M5-W185 $375 HS-25M5-W185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmHS-03M3-W185 $320 HS-05M3-W185 $330 HS-10M3-W185 $350 HS-15M3-W185 $375 HS-25M3-W185 $400
HAISIL HL Microbore ColumnsHAISIL HL C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmLS-0301-C185 $320 LS-0501-C185 $330 LS-1001-C185 $350 LS-1501-C185 $375 LS-2501-C185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmLS-03M8-C185 $320 LS-05M8-C185 $330 LS-10M8-C185 $350 LS-15M8-C185 $375 LS-25M8-C185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmLS-03M5-C185 $320 LS-05M5-C185 $330 LS-10M5-C185 $350 LS-15M5-C185 $375 LS-25M5-C185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmLS-03M3-C185 $320 LS-05M3-C185 $330 LS-10M3-C185 $350 LS-15M3-C185 $375 LS-25M3-C185 $400
HEAVY Microbore Columns HEAVY C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmVS-0301-C185 $320 VS-0501-C185 $330 VS-1001-C185 $350 VS-1501-C185 $375 VS-2501-C185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmVS-03M8-C185 $320 VS-05M8-C185 $330 VS-10M8-C185 $350 VS-15M8-C185 $375 VS-25M8-C185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmVS-03M5-C185 $320 VS-05M5-C185 $330 VS-10M5-C185 $350 VS-15M5-C185 $375 VS-25M5-C185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmVS-03M3-C185 $320 VS-05M3-C185 $330 VS-10M3-C185 $350 VS-15M3-C185 $375 VS-25M3-C185 $400
Microbore HPLC Columns (continued)Wide Range of 1mm, 0.5mm, and 0.3mm Columns
MICROBORE HPLC COLUMN ORDER ING INFORMAT ION
33
PROTO 200 Microbore ColumnsPROTO 200 C4 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmRS-0301-D045 $320 RS-0501-D045 $330 RS-1001-D045 $350 RS-1501-D045 $375 RS-2501-D045 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmRS-03M8-D185 $320 RS-05M8-D185 $330 RS-10M8-D185 $350 RS-15M8-D185 $375 RS-25M8-D185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmRS-03M5-D045 $320 RS-05M5-D045 $330 RS-10M5-D045 $350 RS-15M5-D045 $375 RS-25M5-D045 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmRS-03M3-D045 $320 RS-05M3-D045 $330 RS-10M3-D045 $350 RS-15M3-D045 $375 RS-25M3-D045 $400
PROTO 200 C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmRS-0301-D185 $320 RS-0501-D185 $330 RS-1001-D185 $350 RS-1501-D185 $375 RS-2501-D185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmRS-03M8-D185 $320 RS-05M8-D185 $330 RS-10M8-D185 $350 RS-15M8-D185 $375 RS-25M8-D185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmRS-03M5-D185 $320 RS-05M5-D185 $330 RS-10M5-D185 $350 RS-15M5-D185 $375 RS-25M5-D185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmRS-03M3-D185 $320 RS-05M3-D185 $330 RS-10M3-D185 $350 RS-15M3-D185 $375 RS-25M3-D185 $400
PROTO 300 Microbore ColumnsPROTO 300 C4 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmRS-0301-W045 $320 RS-0501-W045 $330 RS-1001-W045 $350 RS-1501-W045 $375 RS-2501-W045 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmRS-03M8-W045 $320 RS-05M8-W045 $330 RS-10M8-W045 $350 RS-15M8-W045 $375 RS-25M8-W045 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmRS-03M5-W045 $320 RS-05M5-W045 $330 RS-10M5-W045 $350 RS-15M5-W045 $375 RS-25M5-W045 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmRS-03M3-W045 $320 RS-05M3-W045 $330 RS-10M3-W045 $350 RS-15M3-W045 $375 RS-25M3-W045 $400
PROTO 300 C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmRS-0301-W185 $320 RS-0501-W185 $330 RS-1001-W185 $350 RS-1501-W185 $375 RS-2501-W185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmRS-03M8-W185 $320 RS-05M8-W185 $330 RS-10M8-W185 $350 RS-15M8-W185 $375 RS-25M8-W185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmRS-03M5-W185 $320 RS-05M5-W185 $330 RS-10M5-W185 $350 RS-15M5-W185 $375 RS-25M5-W185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmRS-03M3-W185 $320 RS-05M3-W185 $330 RS-10M3-W185 $350 RS-15M3-W185 $375 RS-25M3-W185 $400
PHALANX Microbore Columns (5µm PHALANX on following page)PHALANX C18 3µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmPS-0301-C183 $380 PS-0501-C183 $390 PS-1001-C183 $440 PS-1501-C183 $475 PS-2501-C183 $50030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmPS-03M8-C183 $380 PS-05M8-C183 $390 PS-10M8-C183 $440 PS-15M8-C183 $475 PS-25M8-C183 $50030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmPS-03M5-C183 $380 PS-05M5-C183 $390 PS-10M5-C183 $440 PS-15M5-C183 $475 PS-25M5-C183 $50030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmPS-03M3-C183 $380 PS-05M3-C183 $390 PS-10M3-C183 $440 PS-15M3-C183 $475 PS-25M3-C183 $500
MICROBORE HPLC COLUMN ORDER ING INFORMAT ION
NEW 3µm
200
34
PHALANX Microbore Columns (cont. - 3µm PHALANX on previous page)PHALANX C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmPS-0301-C185 $320 PS-0501-C185 $330 PS-1001-C185 $350 PS-1501-C185 $375 PS-2501-C185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmPS-03M8-C185 $320 PS-05M8-C185 $330 PS-10M8-C185 $350 PS-15M8-C185 $375 PS-25M8-C185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmPS-03M5-C185 $320 PS-05M5-C185 $330 PS-10M5-C185 $350 PS-15M5-C185 $375 PS-25M5-C185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmPS-03M3-C185 $320 PS-05M3-C185 $330 PS-10M3-C185 $350 PS-15M3-C185 $375 PS-25M3-C185 $400
TARGA Microbore Columns TARGA C8 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns
30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmTS-0301-C085 $320 TS-0501-C085 $330 TS-1001-C085 $350 TS-1501-C085 $375 TS-2501-C085 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmTS-03M8-C085 $320 TS-05M8-C085 $330 TS-10M8-C085 $350 TS-15M8-C085 $375 TS-25M8-C085 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmTS-03M5-C085 $320 TS-05M5-C085 $330 TS-10M5-C085 $350 TS-15M5-C085 $375 TS-25M5-C085 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmTS-03M3-C085 $320 TS-05M3-C085 $330 TS-10M3-C085 $350 TS-15M3-C085 $375 TS-25M3-C085 $400
TARGA C18 3µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmTS-0301-C183 $370 TS-0501-C183 $380 TS-1001-C183 $400 TS-1501-C183 $430 TS-2501-C183 $45530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmTS-03M8-C183 $370 TS-05M8-C183 $380 TS-10M8-C183 $400 TS-15M8-C183 $430 TS-25M8-C183 $45530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmTS-03M5-C183 $370 TS-05M5-C183 $380 TS-10M5-C183 $400 TS-15M5-C183 $430 TS-25M5-C183 $45530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmTS-03M3-C183 $370 TS-05M3-C183 $380 TS-10M3-C183 $400 TS-15M3-C183 $430 TS-25M3-C183 $455
TARGA C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmTS-0301-C185 $320 TS-0501-C185 $330 TS-1001-C185 $350 TS-1501-C185 $375 TS-2501-C185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmTS-03M8-C185 $320 TS-05M8-C185 $330 TS-10M8-C185 $350 TS-15M8-C185 $375 TS-25M8-C185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmTS-03M5-C185 $320 TS-05M5-C185 $330 TS-10M5-C185 $350 TS-15M5-C185 $375 TS-25M5-C185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmTS-03M3-C185 $320 TS-05M3-C185 $330 TS-10M3-C185 $350 TS-15M3-C185 $375 TS-25M3-C185 $400
Microbore HPLC Columns (continued)Wide Range of 1mm, 0.5mm, and 0.3mm Columns
MICROBORE HPLC COLUMN ORDER ING INFORMAT ION
Higgins Analytical manufacturers the widest range of Microbore HPLC Columns in the world.
35
MICROBORE POLYMER IC COLUMN ORDER ING INFORMAT ION
Comparison of a Higgins to Another Manufacturer’s 1mm Column
Vydac C18 238MS5115150 x 1.0mm
PROTO 200 C18150 x 1.0mm
Tryptic Digest of Carbamidomethylated BSA (see page 15 for details)
S EM I - PREP AND PREP COLUMNS ORDER ING INFORMAT ION
36
CLIPEUS 10mm Semi-Prep and 20mm Prep ColumnsCLIPEUS C8 5µm 10 and 20mm I.D. s.s. columns
50x10mm 100x10mm 150x10mm 250x10mmCS-0510-C085 $525 CS-1010-C085 $700 CS-1510-C085 $980 CS-2510-C085 $119050x20mm 100x20mm 150x20mm 250x20mmCS-0520-C085 $1200 CS-1020-C085 $1715 CS-1520-C085 $2250 CS-2520-C085 $3300
CLIPEUS C18 10µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmCS-0510-C181 $320 CS-1010-C181 $395 CS-1510-C181 $565 CS-2510-C181 $71550x20mm 100x20mm 150x20mm 250x20mmCS-0520-C181 $950 CS-1020-C181 $1220 CS-1520-C181 $1560 CS-2520-C181 $1990
CLIPEUS C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmCS-0510-C185 $525 CS-1010-C185 $700 CS-1510-C185 $980 CS-2510-C185 $119050x20mm 100x20mm 150x20mm 250x20mmCS-0520-C185 $1200 CS-1020-C185 $1715 CS-1520-C185 $2250 CS-2520-C185 $3300
CLIPEUS Silica 10µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmCS-0510-SIL1 $300 CS-1010-SIL1 $370 CS-1510-SIL1 $530 CS-2510-SIL1 $67050x20mm 100x20mm 150x20mm 250x20mmCS-0520-SIL1 $890 CS-1020-SIL1 $1140 CS-1520-SIL1 $1460 CS-2520-SIL1 $1850
Semi-Prep (10mm) and Prep (20mm)ColumnsNot only does Higgins Analytical manufacturersemi-prep and prep columns with our mostpopular phases, we also produce guardcartridges for these columns. See pages 56 - 57 (10mm) and 60 - 61(20mm) for more information about guardcartridges, and pages 62 - 63 for cartridgeholders.
Guide to Semi-Prep and Prep Column Part NumbersxS-xx10-xxxx 10mm I.D. columnsxS-xx20-xxxx 20mm I.D. columnsSee Page 23 for complete Part Number information
Semi-Prep and Prep ColumnsWide Range of 10mm and 20mm i.d. columns
S EM I - PREP AND PREP COLUMNS ORDER ING INFORMAT ION
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HAISIL100 10mm Semi-Prep and 20mm Prep ColumnsHAISIL 100 C8 5µm 10 and 20mm I.D. s.s. columns
50x10mm 100x10mm 150x10mm 250x10mmHS-0510-M085 $525 HS-1010-M085 $700 HS-1510-M085 $980 HS-2510-M085 $119050x20mm 100x20mm 150x20mm 250x20mmHS-0520-M085 $1200 HS-1020-M085 $1715 HS-1520-M085 $2250 HS-2520-M085 $3300
HAISIL 100 C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmHS-0510-M185 $525 HS-1010-M185 $700 HS-1510-M185 $980 HS-2510-M185 $119050x20mm 100x20mm 150x20mm 250x20mmHS-0520-M185 $1200 HS-1020-M185 $1715 HS-1520-M185 $2250 HS-2520-M185 $3300
PHALANX 10mm Semi-Prep and 20mm Prep ColumnsPHALANX C18 10µm 10 and 20mm I.D. s.s. columns
50x10mm 100x10mm 150x10mm 250x10mmPS-0510-C181 $320 PS-1010-C181 $395 PS-1510-C181 $565 PS-2510-C181 $71550x20mm 100x20mm 150x20mm 250x20mmPS-0520-C181 $950 PS-1020-C181 $1220 PS-1520-C181 $1560 PS-2520-C181 $1990
PHALANX C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmPS-0510-C185 $545 PS-1010-C185 $700 PS-1510-C185 $980 PS-2510-C185 $119050x20mm 100x20mm 150x20mm 250x20mmPS-0520-C185 $1200 PS-1020-C185 $1715 PS-1520-C185 $2250 PS-2520-C185 $3300
PROTO 200 10mm Semi-Prep and 20mm Prep ColumnsPROTO 200 C4 10µm 10 and 20mm I.D. s.s. columns
50x10mm 100x10mm 150x10mm 250x10mmRS-0510-D041 $320 RS-1010-D041 $395 RS-1510-D041 $565 RS-2510-D041 $71550x20mm 100x20mm 150x20mm 250x20mmRS-0520-D041 $950 RS-1020-D041 $1220 RS-1520-D041 $1560 RS-2520-D041 $1990
PROTO 200 C4 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-D045 $525 RS-1010-D045 $700 RS-1510-D045 $980 RS-2510-D045 $119050x20mm 100x20mm 150x20mm 250x20mmRS-0520-D045 $1200 RS-1020-D045 $1715 RS-1520-D045 $2250 RS-2520-D045 $3300
PROTO 200 C18 10µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-D181 $320 RS-1010-D181 $395 RS-1510-D181 $565 RS-2510-D181 $71550x20mm 100x20mm 150x20mm 250x20mmRS-0520-D181 $950 RS-1020-D181 $1220 RS-1520-D181 $1560 RS-2520-D181 $1990
PROTO 200 C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-D185 $525 RS-1010-D185 $700 RS-1510-D185 $980 RS-2510-D185 $119050x20mm 100x20mm 150x20mm 250x20mmRS-0520-D185 $1200 RS-1020-D185 $1715 RS-1520-D185 $2250 RS-2520-D185 $3300
200
S EM I - PREP AND PREP COLUMNS ORDER ING INFORMAT ION
PROTO 300 10mm Semi-Prep and 20mm Prep ColumnsPROTO 300 C4 10µm 10 and 20mm I.D. s.s. columns
50x10mm 100x10mm 150x10mm 250x10mmRS-0510-W041 $320 RS-1010-W041 $395 RS-1510-W041 $565 RS-2510-W041 $71550x20mm 100x20mm 150x20mm 250x20mmRS-0520-W041 $950 RS-1020-W041 $1220 RS-1520-W041 $1560 RS-2520-W041 $1990
PROTO 300 C4 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-W045 $525 RS-1010-W045 $700 RS-1510-W045 $980 RS-2510-W045 $119050x20mm 100x20mm 150x20mm 250x20mmRS-0520-W045 $1200 RS-1020-W045 $1715 RS-1520-W045 $2250 RS-2520-W045 $3300
PROTO 300 C18 10µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-W181 $320 RS-1010-W181 $395 RS-1510-W181 $565 RS-2510-W181 $71550x20mm 100x20mm 150x20mm 250x20mmRS-0520-W181 $950 RS-1020-W181 $1220 RS-1520-W181 $1560 RS-2520-W181 $1990
PROTO 300 C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-W185 $525 RS-1010-W185 $700 RS-1510-W185 $980 RS-2510-W185 $119050x20mm 100x20mm 150x20mm 250x20mmRS-0520-W185 $1200 RS-1020-W185 $1715 RS-1520-W185 $2250 RS-2520-W185 $3300
TARGA 10mm Semi-Prep and 20mm Prep ColumnsTARGA C18 10µm 10 and 20mm I.D. s.s. columns
50x10mm 100x10mm 150x10mm 250x10mmTS-0510-C181 $320 TS-1010-C181 $395 TS-1510-C181 $565 TS-2510-C181 $71550x20mm 100x20mm 150x20mm 250x20mmTS-0520-C181 $950 TS-1020-C181 $1220 TS-1520-C181 $1560 TS-2520-C181 $1990
TARGA C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmTS-0510-C185 $525 TS-1010-C185 $700 TS-1510-C185 $980 TS-2510-C185 $119050x20mm 100x20mm 150x20mm 250x20mmTS-0520-C185 $1200 TS-1020-C185 $1715 TS-1520-C185 $2250 TS-2520-C185 $3300
See pages 56 - 57 and 60 - 61 for information about Guard Cartridges available for these Prep and Semi-Prep Columns
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S EM I - PREP AND PREP COLUMNS ORDER ING INFORMAT ION
Prep Scale (20mm)
Prep Scale (20mm)
Semi-Prep Scale (10mm)
See pages 66 - 67 for information about the Prep and Semi-Prep Cartridge Holders
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40
Robust design with HighPerformance:To the right, aBSA tryptic digest on a150cm x 75µm Targa C18 5µm Caπllary.P/N TC-2507-C185 cut to 15cm.
CAπ L LARY ORDER ING INFORMAT ION
CaπllaryCAPILLARY HPLC COLUMNS
A New Benchmark in Capillary ColumnQuality and Cost:Higgins Analytical’s unique approach pro-vides a flexible format and reasonablypriced capillary HPLC column for LC-MSapplications. Caπllary, π, columns are allmanufactured and tested as 250mmproducts and are offered for the reason-able price of $314.16 for both 75µm and150µm versions.
Cutting and trimming a Caπllary™ Column:The Caπllaries can be used as is, or theycan be easily cut to any required length,with a ceramic cutter that is includedwith each column. Caπllary™ columnsare nominally 25cm in length. Unless thecapillary is going to be used in its fulllength, the white ceramic cutter is usedto cut the column to the required size.Measure from the frit in the center ofthe green section. This technique can alsobe useful for removing a short sectionfrom the column inlet if it becomes con-taminated or plugged. Similarly, the col-umn outlet can be trimmed to a properlength before attaching it to the post-col-umn device.
Plumbing Considerations:The Caπllary™ 75 and Caπllary™ 150columns have standard 360µm outerdiameters which can be convenientlyplumbed to HPLC systems in a widevariety of manners. The optional HigginsMicro Column Connection Kit (P/N MIC-KIT,see page 74) includes sleeves and finger-tight bushings to facilitate capillary col-umn attachment to most injection valves,detectors, and MS source inlets.
π
TM
NEW
$314.16
HAISIL 300 C18 5µm250x0.075mmHC-2507-W185 $314.16250x0.150mmHC-2515-W185 $314.16
HAISIL HL C18 5µm250x0.075mmLC-2507-C185 $314.16250x0.150mmLC-2515-C185 $314.16
HEAVY C18 5µm250x0.075mmVC-2507-C185 $314.16250x0.150mmVC-2515-C185 $314.16
PROTO 200 C4 5µm250x0.075mmRC-2507-D045 $314.16250x0.150mm RC-2515-D045 $314.16
PROTO 200 C18 5µm250x0.075mmRC-2507-D185 $314.16250x0.150mmRC-2515-D185 $314.16
PROTO 300 C4 5µm75µm and 150µm 250x0.075mmRC-2507-W045 $314.16250x0.150mmRC-2515-W045 $314.16
PROTO 300 C18 5µm75µm and 150µm 250x0.075mmRC-2507-W185 $314.16250x0.150mmRC-2515-W185 $314.16
PHALANX C18 5µm75µm and 150µm 250x0.075mmPC-2507-C185 $314.16250x0.150mmPC-2515-C185 $314.16
TARGA C8 5µm75µm and 150µm250x0.075mmTC-2507-C085 $314.16250x0.150mmTC-2515-C085 $314.16
TARGA C18 5µm250x0.075mmTC-2507-C185 $314.16250x0.150mmTC-2515-C185 $314.16
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CAπ L LARY ORDER ING INFORMAT ION
200
Caπllary™ is a trademark of Higgins Analytical, Inc.
CLIPEUS C8 5µm250x0.075mmCC-2507-C085 $314.16250x0.150mmCC-2515-C085 $314.16
CLIPEUS C18 5µm250x0.075mmCC-2507-C185 $314.16250x0.150mmCC-2515-C185 $314.16
CLIPEUS CYANO 5µm250x0.075mmCC-2507-CNP5 $314.16250x0.150mmCC-2515-CNP5 $314.16
CLIPEUS PHENYL 5µm250x0.075mmCC-2507-PHN5 $314.16250x0.150mmCC-2515-PHN5 $314.16
CLIPEUS SILICA 5µm250x0.075mmCC-2507-SIL5 $314.16250x0.150mmCC-2515-SIL5 $314.16
HAISIL 100 C8 5µm250x0.075mmHC-2507-M085 $314.16250x0.150mmHC-2515-M085 $314.16
HAISIL 100 C18 5µm250x0.075mmHC-2507-M185 $314.16250x0.150mmHC-2515-M185 $314.16
HAISIL 100 SILICA 5µm250x0.075mmHC-2507-SIL5 $314.16250x0.150mmHC-2515-SIL5 $314.16
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CAPELLINI 6-32CAPILLARY HPLC COLUMNS with 6-32 Thread PEEK/Ti Fittings
Higgins Analytical Capellini are robust,high performance capillary HPLCcolumns for LC-MS and other very smallscale applications. The inert PEEK andtitanium fittings complement the HigginsAnalytical sorbents such as TARGAwhich was developed to enable LC-MSapplications without TFA.
Our no nonsense testing proceduredoes not resort to sample and flowsplitting techniques, inaccurate k’ values,and miniscule chromatograms, as dosome manufacturers, to illustrate columnperformance. The CofA’s symmetricalpeaks and the high resolution map speakfor themselves.
Capellini - Back by Popular DemandWhen Higgins Analytical introducedCaπllary columns three years ago, weremoved Capellini from our catalog,however, we contnued to manufacturethis design for customers that had beenusing it for several years. Somewhat toour surprise, Capellini sales havecontinued to grow and we still receivenumerous product inquiries in spite oftheir absence from our literature. WhileCaπllary columns are very efficient andcost effective (see page 40), we havefound that many chromatographersprefer the symmetrical, flow reversabledesign and the 6-32 threaded end-fittingsof Capellini. Even though Capellini aremore expensive to manufacture andsomewhat less efficient than Caπllarycolumns, due to the end fitting design,they enjoy a wide variety of uses thatinvolve flow reversal, such trap applica-tions.
Guide to CAPELLINI Part NumbersxFS-xx07-xxxx 75µm CAPELLINI 6-32xFS-xx15-xxxx 150µm CAPELLINI 6-32See Page 23 for complete Part Number information
Capillary HPLC ColumnsUnique Features:• 6-32 threads for capillary fittings• PEEK/Titanium fittings• Tested and supplied with CofA• High performance• Higgins Analytical Sorbents
Applications:• LC-MS• Microbore HPLC
Standard Sizes:• 50 to 150 x 0.150mm• 50 to 150 x 0.075mm• Longer and shorter lengths available
High Selectivity:A partial 1.5µL/min LCMSMS map of aBSA tryptic digest.
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CLIPEUS Capillary Columns with 6-32 threadsCLIPEUS C8 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmCFS-0507-C085 $400 CFS-1007-C085 $400 CFS-1507-C085 $40050x0.150mm 100x0.150mm 150x0.150mmCFS-0515-C085 $400 CFS-1015-C085 $400 CFS-1515-C085 $400
CLIPEUS C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCFS-0507-C185 $400 CFS-1007-C185 $400 CFS-1507-C185 $40050x0.150mm 100x0.150mm 150x0.150mmCFS-0515-C185 $400 CFS-1015-C185 $400 CFS-1515-C185 $400
CLIPEUS Cyano 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCFS-0507-CNP5 $400 CFS-1007-CNP5 $400 CFS-1507-CNP5 $40050x0.150mm 100x0.150mm 150x0.150mmCFS-0515-CNP5 $400 CFS-1015-CNP5 $400 CFS-1515-CNP5 $400
CLIPEUS Phenyl 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCFS-0507-PHN5 $400 CFS-1007-PHN5 $400 CFS-1507-PHN5 $40050x0.150mm 100x0.150mm 150x0.150mmCFS-0515-PHN5 $400 CFS-1015-PHN5 $400 CFS-1515-PHN5 $400
CLIPEUS Silica 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCFS-0507-SIL5 $400 CFS-1007-SIL5 $400 CFS-1507-SIL5 $40050x0.150mm 100x0.150mm 150x0.150mmCFS-0515-SIL5 $400 CFS-1015-SIL5 $400 CFS-1515-SIL5 $400
HAISIL100 Capillary Columns with 6-32 threadsHAISIL 100 C8 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmHFS-0507-M085 $400 HFS-1007-M085 $400 HFS-1507-M085 $40050x0.150mm 100x0.150mm 150x0.150mmHFS-0515-M085 $400 HFS-1015-M085 $400 HFS-1515-M085 $400
HAISIL 100 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmHFS-0507-M185 $400 HFS-1007-M185 $400 HFS-1507-M185 $40050x0.150mm 100x0.150mm 150x0.150mmHFS-0515-M185 $400 HFS-1015-M185 $400 HFS-1515-M185 $400
HAISIL 100 Silica 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmHFS-0507-SIL5 $400 HFS-1007-SIL5 $400 HFS-1507-SIL5 $40050x0.150mm 100x0.150mm 150x0.150mmHFS-0515-SIL5 $400 HFS-1015-SIL5 $400 HFS-1515-SIL5 $400
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HAISIL300 Capillary Columns with 6-32 threadsHAISIL 300 C18 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmHFS-0507-W185 $400 HFS-1007-W185 $400 HFS-1507-W185 $40050x0.150mm 100x0.150mm 150x0.150mmHFS-0515-W185 $400 HFS-1015-W185 $400 HFS-1515-W185 $400
HAISIL HL Capillary Columns with 6-32 threadsHAISIL HL C18 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmLFS-0507-C185 $400 LFS-1007-C185 $400 LFS-1507-C185 $40050x0.150mm 100x0.150mm 150x0.150mmLFS-0515-C185 $400 LFS-1015-C185 $400 LFS-1515-C185 $400
HEAVY Capillary Columns with 6-32 threads HEAVY C18 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmVFS-0507-C185 $400 VFS-1007-C185 $400 VFS-1507-C185 $40050x0.150mm 100x0.150mm 150x0.150mmVFS-0515-C185 $400 VFS-1015-C185 $400 VFS-1515-C185 $400
PROTO 200 Capillary Columns with 6-32 threadsPROTO 200 C4 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmRFS-0507-D045 $400 RFS-1007-D045 $400 RFS-1507-D045 $40050x0.150mm 100x0.150mm 150x0.150mmRFS-0515-D045 $400 RFS-1015-D045 $400 RFS-1515-D045 $400
PROTO 200 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmRFS-0507-D185 $400 RFS-1007-D185 $400 RFS-1507-D185 $40050x0.150mm 100x0.150mm 150x0.150mmRFS-0515-D185 $400 RFS-1015-D185 $400 RFS-1515-D185 $400
CAPELLINI 6-32 (cont.)CAPILLARY HPLC COLUMNS with 6-32 Thread PEEK/Ti Fittings
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PROTO 300 Capillary Columns with 6-32 threadsPROTO 300 C4 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmRFS-0507-W045 $400 RFS-1007-W045 $400 RFS-1507-W045 $40050x0.150mm 100x0.150mm 150x0.150mmRFS-0515-W045 $400 RFS-1015-W045 $400 RFS-1515-W045 $400
PROTO 300 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmRFS-0507-W185 $400 RFS-1007-W185 $400 RFS-1507-W185 $40050x0.150mm 100x0.150mm 150x0.150mmRFS-0515-W185 $400 RFS-1015-W185 $400 RFS-1515-W185 $400
PHALANX Capillary Columns with 6-32 threadsPHALANX C18 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmPFS-0507-C185 $400 PFS-1007-C185 $400 PFS-1507-C185 $40050x0.150mm 100x0.150mm 150x0.150mmPFS-0515-C185 $400 PFS-1015-C185 $400 PFS-1515-C185 $400
TARGA Capillary Columns with 6-32 threadsTARGA C8 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmTFS-0507-C085 $400 TFS-1007-C085 $400 TFS-1507-C085 $40050x0.150mm 100x0.150mm 150x0.150mmTFS-0515-C085 $400 TFS-1015-C085 $400 TFS-1515-C085 $400
TARGA C18 3µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmTFS-0507-C183 $400 TFS-1007-C183 $400 TFS-1507-C183 $40050x0.150mm 100x0.150mm 150x0.150mmTFS-0515-C183 $400 TFS-1015-C183 $400 TFS-1515-C183 $400
TARGA C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmTFS-0507-C185 $400 TFS-1007-C185 $400 TFS-1507-C185 $40050x0.150mm 100x0.150mm 150x0.150mmTFS-0515-C185 $400 TFS-1015-C185 $400 TFS-1515-C185 $400
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CAPELLINI 10-32CAPILLARY HPLC COLUMNS with 10-32 Thread s.s. Fittings
Higgins Analytical Capellini have beenpopular with chromatographers requiringan easy-to-use and robust capillaryHPLC column. The original Capellini(now called Capellini 6-32) wereavailable only with 6-32 fittings. Now, wehave another version for chromatogra-phers prefering 10-32 thread fittings -Capellini 10-32. Another differencebetween the two versions of Capellini isthat the 10-32 threads are stainless steelrather than PEEK. Both types are madewith titanium frits on each end, providinginertness and flow direction reversability.
Guide to CAPELLINI 10-32 Part NumbersxPS-xx07-xxxx 75µm CAPELLINI 10-32xPS-xx15-xxxx 150µm CAPELLINI 10-32See Page 23 for complete Part Number information
Capillary HPLC ColumnsUnique Features:• 10-32 threads for standard fittings• s.s. End Fittings/Titanium Frits• Tested and supplied with CofA• High performance• Higgins Analytical Sorbents
Applications:• LC-MS• Microbore HPLC
Standard Sizes:• 50 to 150 x 0.150mm• 50 to 150 x 0.075mm• Longer and shorter lengths available
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NEW
Robust design with HighPerformance:To the right, aBSA tryptic digest on a150cm x 75µm Targa C18 5µm Capellini.P/N TC-1507-C185
CAPELL IN I 1 0 - 3 2 ORDER ING INFORMAT ION
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CLIPEUS Capillary Columns with 10-32 threadsCLIPEUS C8 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmCPS-0507-C085 $450 CPS-1007-C085 $450 CPS-1507-C085 $45050x0.150mm 100x0.150mm 150x0.150mmCPS-0515-C085 $450 CPS-1015-C085 $450 CPS-1515-C085 $450
CLIPEUS C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCPS-0507-C185 $450 CPS-1007-C185 $450 CPS-1507-C185 $45050x0.150mm 100x0.150mm 150x0.150mmCPS-0515-C185 $450 CPS-1015-C185 $450 CPS-1515-C185 $450
CLIPEUS Cyano 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCPS-0507-CNP5 $450 CPS-1007-CNP5 $450 CPS-1507-CNP5 $45050x0.150mm 100x0.150mm 150x0.150mmCPS-0515-CNP5 $450 CPS-1015-CNP5 $450 CPS-1515-CNP5 $450
CLIPEUS Phenyl 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCPS-0507-PHN5 $450 CPS-1007-PHN5 $450 CPS-1507-PHN5 $45050x0.150mm 100x0.150mm 150x0.150mmCPS-0515-PHN5 $450 CPS-1015-PHN5 $450 CPS-1515-PHN5 $450
CLIPEUS Silica 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCPS-0507-SIL5 $450 CPS-1007-SIL5 $450 CPS-1507-SIL5 $45050x0.150mm 100x0.150mm 150x0.150mmCPS-0515-SIL5 $450 CPS-1015-SIL5 $450 CPS-1515-SIL5 $450
HAISIL100 Capillary Columns with 10-32 threadsHAISIL 100 C8 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmHPS-0507-M085 $450 HPS-1007-M085 $450 HPS-1507-M085 $45050x0.150mm 100x0.150mm 150x0.150mmHPS-0515-M085 $450 HPS-1015-M085 $450 HPS-1515-M085 $450
HAISIL 100 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmHPS-0507-M185 $450 HPS-1007-M185 $450 HPS-1507-M185 $45050x0.150mm 100x0.150mm 150x0.150mmHPS-0515-M185 $450 HPS-1015-M185 $450 HPS-1515-M185 $450
HAISIL 100 Silica 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmHPS-0507-SIL5 $450 HPS-1007-SIL5 $450 HPS-1507-SIL5 $45050x0.150mm 100x0.150mm 150x0.150mmHPS-0515-SIL5 $450 HPS-1015-SIL5 $450 HPS-1515-SIL5 $450
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HAISIL300 Capillary Columns with 10-32 threadsHAISIL 300 C18 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmHPS-0507-W185 $450 HPS-1007-W185 $450 HPS-1507-W185 $45050x0.150mm 100x0.150mm 150x0.150mmHPS-0515-W185 $450 HPS-1015-W185 $450 HPS-1515-W185 $450
HAISIL HL Capillary Columns with 10-32 threadsHAISIL HL C18 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmLPS-0507-C185 $450 LPS-1007-C185 $450 LPS-1507-C185 $45050x0.150mm 100x0.150mm 150x0.150mmLPS-0515-C185 $450 LPS-1015-C185 $450 LPS-1515-C185 $450
HEAVY Capillary Columns with 10-32 threads HEAVY C18 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmVPS-0507-C185 $450 VPS-1007-C185 $450 VPS-1507-C185 $45050x0.150mm 100x0.150mm 150x0.150mmVPS-0515-C185 $450 VPS-1015-C185 $450 VPS-1515-C185 $450
PROTO 200 Capillary Columns with 10-32 threadsPROTO 200 C4 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmRPS-0507-D045 $450 RPS-1007-D045 $450 RPS-1507-D045 $45050x0.150mm 100x0.150mm 150x0.150mmRPS-0515-D045 $450 RPS-1015-D045 $450 RPS-1515-D045 $450
PROTO 200 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmRPS-0507-D185 $450 RPS-1007-D185 $450 RPS-1507-D185 $45050x0.150mm 100x0.150mm 150x0.150mmRPS-0515-D185 $450 RPS-1015-D185 $450 RPS-1515-D185 $450
CAPELLINI 10-32 (cont.)CAPILLARY HPLC COLUMNS with 10-32 Thread s.s. Fittings
CAPELL IN I 1 0 - 3 2 ORDER ING INFORMAT ION
49
PROTO 300 Capillary Columns with 10-32 threadsPROTO 300 C4 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmRPS-0507-W045 $450 RPS-1007-W045 $450 RPS-1507-W045 $45050x0.150mm 100x0.150mm 150x0.150mmRPS-0515-W045 $450 RPS-1015-W045 $450 RPS-1515-W045 $450
PROTO 300 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmRPS-0507-W185 $450 RPS-1007-W185 $450 RPS-1507-W185 $45050x0.150mm 100x0.150mm 150x0.150mmRPS-0515-W185 $450 RPS-1015-W185 $450 RPS-1515-W185 $450
PHALANX Capillary Columns with 10-32 threadsPHALANX C18 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmPPS-0507-C185 $450 PPS-1007-C185 $450 PPS-1507-C185 $45050x0.150mm 100x0.150mm 150x0.150mmPPS-0515-C185 $450 PPS-1015-C185 $450 PPS-1515-C185 $450
TARGA Capillary Columns with 10-32 threadsTARGA C8 5µm 75µm and 150µm Capillary columns
50x0.075mm 100x0.075mm 150x0.075mmTPS-0507-C085 $450 TPS-1007-C085 $450 TPS-1507-C085 $45050x0.150mm 100x0.150mm 150x0.150mmTPS-0515-C085 $450 TPS-1015-C085 $450 TPS-1515-C085 $450
TARGA C18 3µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmTPS-0507-C183 $450 TPS-1007-C183 $450 TPS-1507-C183 $45050x0.150mm 100x0.150mm 150x0.150mmTPS-0515-C183 $450 TPS-1015-C183 $450 TPS-1515-C183 $450
TARGA C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmTPS-0507-C185 $450 TPS-1007-C185 $450 TPS-1507-C185 $45050x0.150mm 100x0.150mm 150x0.150mmTPS-0515-C185 $450 TPS-1015-C185 $450 TPS-1515-C185 $450
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S PR ITE ORDER ING INFORMAT ION
SPRITEDIRECT CONNECT HPLC COLUMN
The Sprite family of narrowbore HPLCcolumns is a unique high performanceproduct for LC-MS and other high-speed, small volume applications. Allthree sizes of 2.1mm I.D Sprite HPLCcolumns are individually tested. ACertificate of Analysis accompanies eachcolumn. The Sprite’s certificate includesthe original chromatogram and compre-hensive testing and sorbentdocumentation.
The inert PEEK and titanium design hasbeen proven to be structurally sound toover 5,000 PSI (340Bar).
The optional Higgins Micro ColumnConnection Kit (P/N MIC-KIT, see page 74)includes sleeves and finger-tight bushingsto facilitate the attachment of a Sprite tomost injection valves, detectors, and MSsource inlets.
Guide to SPRITE Part NumbersxR-0121-xxxx 10 x 2.1mm SpritexR-0221-xxxx 20 x 2.1mm SpritexR-0421-xxxx 40 x 2.1mm SpriteSee Page 23 for complete Part Number information
CLIPEUS SpriteCLIPEUS C8 5µm
10X2.1mm 20X2.1mm 40X2.1mmCR-0121-C085 $175 CR-0221-C085 $185 CR-0421-C085 $215
CLIPEUS C18 5µm10X2.1mm 20X2.1mm 40X2.1mmCR-0121-C185 $175 CR-0221-C185 $185 CR-0421-C185 $215
CLIPEUS Cyano 5µm10X2.1mm 20X2.1mm 40X2.1mmCR-0121-CNP5 $175 CR-0221-CNP5 $185 CR-0421-CNP5 $215
CLIPEUS Phenyl 5µm10X2.1mm 20X2.1mm 40X2.1mmCR-0121-PHN5 $175 CR-0221-PHN5 $185 CR-0421-PHN5 $215
CLIPEUS Silica 5µm10X2.1mm 20X2.1mm 40X2.1mmCR-0121-SIL5 $175 CR-0221-SIL5 $185 CR-0421-SIL5 $215
HAISIL 100 SpriteHAISIL 100 C8 5µm
10X2.1mm 20X2.1mm 40X2.1mmHR-0121-M085 $175 HR-0221-M085 $185 HR-0421-M085 $215
HAISIL 100 C18 5µm10X2.1mm 20X2.1mm 40X2.1mmHR-0121-M185 $175 HR-0221-M185 $185 HR-0421-M185 $215
HAISIL 300 SpriteHAISIL 300 C18 5µm
10X2.1mm 20X2.1mm 40X2.1mmHR-0121-W185 $175 HR-0221-W185 $185 HR-0421-W185 $215
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S PR ITE ORDER ING INFORMAT ION
HAISIL HL SpriteHAISIL HL C18 5µm
10X2.1mm 20X2.1mm 40X2.1mmLR-0121-C185 $175 LR-0221-C185 $185 LR-0421-C185 $215
HEAVY SpriteHEAVY C18 5µm
10X2.1mm 20X2.1mm 40X2.1mmVR-0121-C185 $175 VR-0221-C185 $185 VR-0421-C185 $215
PHALANX SpritePHALANX C18 5µm
10X2.1mm 20X2.1mm 40X2.1mmPR-0121-C185 $175 PR-0221-C185 $185 PR-0421-C185 $215
PROTO 200 SpritePROTO 200 C4 5µm
10X2.1mm 20X2.1mm 40X2.1mmRR-0121-D045 $175 RR-0221-D045 $185 RR-0421-D045 $215
PROTO 200 C18 5µm10X2.1mm 20X2.1mm 40X2.1mmRR-0121-D185 $175 RR-0221-D185 $185 RR-0421-D185 $215
PROTO 300 SpritePROTO 300 C4 5µm
10X2.1mm 20X2.1mm 40X2.1mmRR-0121-W045 $175 RR-0221-W045 $185 RR-0421-W045 $215
PROTO 300 C18 5µm10X2.1mm 20X2.1mm 40X2.1mmRR-0121-W185 $175 RR-0221-W185 $185 RR-0421-W185 $215
TARGA SpriteTARGA C8 5µm
10X2.1mm 20X2.1mm 40X2.1mmTR-0121-C085 $175 TR-0221-C085 $185 TR-0421-C085 $215
TARGA C18 5µm10X2.1mm 20X2.1mm 40X2.1mmTR-0121-C185 $175 TR-0221-C185 $185 TR-0421-C185 $215
SPRITE is Designed to Operate inEither Flow DirectionA SPRITE is made of PEEK and titaniumand can be connected directly to asample injection valve or directly to amass spectrometer’s source inlet.
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52
The Piccolo family of microbore HPLCcolumns is a unique high performanceproduct for LC-MS and other high-speed, small volume applications. Theseefficient columns are the only productsof their type that are individually tested.A Certificate of Analysis accompanieseach column.
In addition to analytical microboreapplications Piccolo columns are idealguards for protecting longer microboreHPLC columns.
Piccolo column void volumes range fromapproximately 5µl to 300nL, makingthem perfect for a wide variety oftrapping, desalting, and trace enrichmentapplications. These small columns can beconveniently used with micro injectionand switching valves as pictured on theleft.
The optional Higgins Micro ColumnConnection Kit (P/N MIC-KIT, see page 74)includes sleeves and finger-tight bushingsto facilitate the attachment of a Piccoloto most injection valves, detectors, andMS source inlets.
PICCOLOSHORT MICROBORE TRAPS AND COLUMNS
Guide to PICCOLO Part NumbersxP-M2M3-xxxx 2.5 x 0.3mm PICCOLOxP-M2M5-xxxx 2.5 x 0.5mm PICCOLOxP-M501-xxxx 5 x 1mm PICCOLOxP-0101-xxxx 10 x 1mm PICCOLOxP-0301-xxxx 30 x 1mm PICCOLOSee Page 23 for complete Part Number information
CLIPEUS PiccoloCLIPEUS C8 5µm
2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmCP-M2M3-C085 $175 CP-M2M5-C085 $175 CP-M501-C085 $175 CP-0101-C085 $175 CP-0301-C085 $220
CLIPEUS C18 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmCP-M2M3-C185 $175 CP-M2M5-C185 $175 CP-M501-C185 $175 CP-0101-C185 $175 CP-0301-C185 $220
CLIPEUS Cyano 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmCP-M2M3-CNP5 $175 CP-M2M5-CNP5 $175 CP-M501-CNP5 $175 CP-0101-CNP5 $175 CP-0301-CNP5 $220
CLIPEUS Phenyl 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmCP-M2M3-PHN5 $175 CP-M2M5-PHN5 $175 CP-M501-PHN5 $175 CP-0101-PHN5 $175 CP-0301-PHN5 $220
CLIPEUS Silica 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmCP-M2M3-SIL5 $175 CP-M2M5-SIL5 $175 CP-M501-SIL5 $175 CP-0101-SIL5 $175 CP-0301-SIL5 $220
HAISIL 100 PiccoloHAISIL 100 C8 5µm
2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmHP-M2M3-M085 $175 HP-M2M5-M085 $175 HP-M501-M085 $175 HP-0101-M085 $175 HP-0301-M085 $220
HAISIL 100 C18 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmHP-M2M3-M185 $175 HP-M2M5-M185 $175 HP-M501-M185 $175 HP-0101-M185 $175 HP-0301-M185 $220
HAISIL 300 PiccoloHAISIL 300 C18 5µm
2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmHP-M2M3-W185 $175 HP-M2M5-W185 $175 HP-M501-W185 $175 HP-0101-W185 $175 HP-0301-W185 $220
P ICCOLO ORDER ING INFORMAT ION
NEW
53
HAISIL HL PiccoloHAISIL HL C18 5µm
2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmLP-M2M3-C185 $175 LP-M2M5-C185 $175 LP-M501-C185 $175 LP-0101-C185 $175 LP-0301-C185 $220
HEAVY PiccoloHEAVY C18 5µm
2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmVP-M2M3-C185 $175 VP-M2M5-C185 $175 VP-M501-C185 $175 VP-0101-C185 $175 VP-0301-C185 $220
PHALANX PiccoloPHALANX C18 5µm
2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmPP-M2M3-C185 $175 PP-M2M5-C185 $175 PP-M501-C185 $175 PP-0101-C185 $175 PP-0301-C185 $220
PROTO 200 PiccoloPROTO 200 C4 5µm
2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmRP-M2M3-D045 $175 RP-M2M5-D045 $175 RP-M501-D045 $175 RP-0101-D045 $175 RP-0301-D045 $220
PROTO 200 C18 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmRP-M2M3-D185 $175 RP-M2M5-D185 $175 RP-M501-D185 $175 RP-0101-D185 $175 RP-0301-D185 $220
PROTO 300 PiccoloPROTO 300 C4 5µm
2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmRP-M2M3-W045 $175 RP-M2M5-W045 $175 RP-M501-W045 $175 RP-0101-W045 $175 RP-0301-WO45 $220
PROTO 300 C18 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmRP-M2M3-W185 $175 RP-M2M5-W185 $175 RP-M501-W185 $175 RP-0101-W185 $175 RP-0301-W185 $220
TARGA PiccoloTARGA C8 5µm
2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmTP-M2M3-C085 $175 TP-M2M5-C085 $175 TP-M501-C085 $175 TP-0101-C085 $175 TP-0301-C085 $220
TARGA C18 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmTP-M2M3-C185 $175 TP-M2M5-C185 $175 TP-M501-C185 $175 TP-0101-C185 $175 TP-0301-C185 $220
PICCOLO (continued)SHORT MICROBORE COLUMNS
P ICCOLO ORDER ING INFORMAT ION
200
NEW
30x1mm
For 3 tim
es greater
trapping capacity
54
HAICART CARTRIDGESSTAINLESS STEEL CARTRIDGE COLUMNS
The HAICART family of HPLC cartridgecolumns provides a convenient andeconomical approach to guard column,trace enrichment, and fast analysisapplications. These stainless steelcartridges are available with 3.2mm and4.6mm internal diameters and with 2, 4,5, or 10cm lengths. This wide selectionmakes it possible to address numerousanalytical needs. See pages 66 - 67 for information aboutthe cartridge holders required forHiggins Analytical’s HAICART CartridgeColumns. HAICART cartridges are alsocompatible with PE/Brownlee MPLCcartridge holders.
Guide to HAICART Analytical Cartridge Part NumbersxF-0421-xxxx 40 x 3.2mm stainless steel cartridgexF-0532-xxxx 50 x 3.2mm stainless steel cartridgexF-1032-xxxx 100 x 3.2mm stainless steel cartridgexF-0446-xxxx 40 x 4.6mm stainless steel cartridgexF-0546-xxxx 50 x 4.6mm stainless steel cartridgexF-1046-xxxx 100 x 4.6mm stainless steel cartridgeSee Page 23 for complete Part Number information
HA ICART STA INLE S S STEE L CARTR IDGE COLUMNS
CLIPEUS 3.2 and 4.6mm I.D. S.S.CartridgesCLIPEUS C8 5µm
20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgCF-0232-C085 $175 CF-0432-C085 $290 CF-0532-C085 $305 CF-1032-C085 $205
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgCF-0446-C085 $290 CF-0546-C085 $305 CF-1046-C085 $205
CLIPEUS C18 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgCF-0232-C185 $175 CF-0432-C185 $290 CF-0532-C185 $305 CF-1032-C185 $205
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgCF-0446-C185 $290 CF-0546-C185 $305 CF-1046-C185 $205
CLIPEUS Cyano 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgCF-0232-CNP5 $185 CF-0432-CNP5 $305 CF-0532-CNP5 $320 CF-1032-CNP5 $215
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgCF-0446-CNP5 $305 CF-0546-CNP5 $320 CF-1046-CNP5 $215
CLIPEUS Phenyl 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgCF-0232-PHN5 $185 CF-0432-PHN5 $305 CF-0532-PHN5 $320 CF-1032-PHN5 $215
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgCF-0446-PHN5 $305 CF-0546-PHN5 $320 CF-1046-PHN5 $215
CLIPEUS Silica 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgCF-0232-SIL5 $175 CF-0432-SIL5 $290 CF-0532-SIL5 $305 CF-1032-SIL5 $205
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgCF-0446-SIL5 $290 CF-0546-SIL5 $305 CF-1046-SIL5 $205
HAISIL 100 C8 3.2 and 4.6mm I.D. S.S.CartridgesHAISIL 100 C8 5µm
20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgHF-0232-M085 $175 HF-0432-M085 $290 HF-0532-M085 $305 HF-1032-M085 $205
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgHF-0446-M085 $290 HF-0546-M085 $305 HF-1046-M085 $205
HAISIL 100 C18 3.2 and 4.6mm I.D. S.S.CartridgesHAISIL 100 C18 5µm
20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgHF-0232-M185 $175 HF-0432-M185 $290 HF-0532-M185 $305 HF-1032-M185 $205
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgHF-0446-M185 $290 HF-0546-M185 $305 HF-1046-M185 $205
HAISIL 300 3.2 and 4.6mm I.D. S.S.CartridgesHAISIL 300 C18 5µm
20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgHF-0232-W185 $190 HF-0432-W185 $330 HF-0532-W185 $340 HF-1032-W185 $225
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgHF-0446-W185 $330 HF-0546-W185 $340 HF-1046-W185 $225
HAISIL HL 3.2 and 4.6mm I.D. S.S.CartridgesHAISIL HL C18 5µm
20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgLF-0232-C185 $185 LF-0432-C185 $330 LF-0532-C185 $340 LF-1032-C185 $225
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgLF-0446-C185 $330 LF-0546-C185 $340 LF-1046-C185 $225
HEAVY 3.2 and 4.6mm I.D. S.S.CartridgesHEAVY C18 5µm
20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgVF-0232-C185 $190 VF-0432-C185 $330 VF-0532-C185 $340 VF-1032-C185 $225
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgVF-0446-C185 $330 VF-0546-C185 $340 VF-1046-C185 $225
55
HA ICART STA INLE S S STEE L CARTR IDGE COLUMNS
56
HAICART CARTRIDGES (continued)STAINLESS STEEL CARTRIDGE COLUMNS
HA ICART STA INLE S S STEE L CARTR IDGE COLUMNS
PHALANX 3.2 and 4.6mm I.D. S.S.CartridgesPHALANX C18 5µm
20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgPF-0232-C185 $190 PF-0432-C185 $330 PF-0532-C185 $340 PF-1032-C185 $225
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgPF-0446-C185 $330 PF-0546-C185 $340 PF-1046-C185 $225
PROTO 200 3.2 and 4.6mm I.D. S.S.CartridgesPROTO 200 C4 5µm
20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgRF-0232-D045 $190 RF-0432-D045 $330 RF-0532-D045 $340 RF-1032-D045 $225
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgRF-0446-D045 $330 RF-0546-D045 $340 RF-1046-D045 $225
PROTO 200 C18 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgRF-0232-D185 $190 RF-0432-D185 $330 RF-0532-D185 $340 RF-1032-D185 $225
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgRF-0446-D185 $330 RF-0546-D185 $340 RF-1046-D185 $225
PROTO 300 3.2 and 4.6mm I.D. S.S.CartridgesPROTO 300 C4 5µm
20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgRF-0232-W045 $190 RF-0432-W045 $330 RF-0532-W045 $340 RF-1032-W045 $225
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgRF-0446-W045 $330 RF-0546-W045 $340 RF-1046-W045 $225
PROTO 300 C18 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgRF-0232-W185 $190 RF-0432-W185 $330 RF-0532-W185 $340 RF-1032-W185 $225
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgRF-0446-W185 $330 RF-0546-W185 $340 RF-1046-W185 $225
TARGA 3.2 and 4.6mm I.D. S.S.CartridgesTARGA C8 5µm
20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgTF-0232-C085 $175 TF-0432-C085 $330 TF-0532-C085 $340 TF-1032-C085 $225
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgTF-0446-C085 $330 TF-0546-C085 $340 TF-1046-C085 $225
TARGA C18 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgTF-0232-C185 $175 TF-0432-C185 $330 TF-0532-C185 $340 TF-1032-C185 $225
40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgTF-0446-C185 $330 TF-0546-C185 $340 TF-1046-C185 $225
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57
HA ICART STA INLE S S STEE L CARTR IDGE COLUMNS
See pages 66 - 67 for information about the Cartridge Holders required for these Cartridges
58
HA I P E EK P E EK /T I TAN IUM CARTR IDGE COLUMNS
HAIPEEK CARTRIDGESPEEK/TITANIUM CARTRIDGE COLUMNS
PEEK and Titanium CartridgesThe HAIPEEK family of narrowboreHPLC cartridge columns is a unique highperformance product for LC-MS andother high-speed, small volume applica-tions. The 40 and 50mm sizes of 2.1mmI.D HAIPEEK HPLC Cartridges areindividually tested. A Certificate ofAnalysis, which includes the originalchromatogram and comprehensivetesting and sorbent documentation,accompanies each column. The 20mm x2.1mm cartridges excel as narrowboreguard columns, yet even though they arenot individually tested, the majority areroutinely used as very economical andefficient columns for high speed HPLCscreening and LC-MS applications. Seepages 66 - 67 for information about thecartridge holders required for HigginsAnalytical’s HAIPEEK Cartridge Columns.HAIPEEK cartridges are also compatiblewith PE/Brownlee MPLC cartridgeholders.
Guide to HAIPEEK Analytical Cartridge Part NumbersxK-0421-xxxx 40 x 2.1mm PEEK/titanium cartridgexK-0521-xxxx 50 x 2.1mm PEEK/titanium cartridgeSee Page 23 for complete Part Number information
CLIPEUS 2.1mm I.D. PEEK/Titanium CartridgesCLIPEUS C8 5µm
20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgCK-0221-C085 $180 CK-0421-C085 $270 CK-0521-C085 $275
CLIPEUS C18 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgCK-0221-C185 $180 CK-0421-C185 $270 CK-0521-C185 $275
CLIPEUS Cyano 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgCK-0221-CNP5 $190 CK-0421-CNP5 $285 CK-0521-CNP5 $290
CLIPEUS Phenyl 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgCK-0221-PHN5 $190 CK-0421-PHN5 $285 CK-0521-PHN5 $290
CLIPEUS Silica 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgCK-0221-SIL5 $180 CK-0421-SIL5 $270 CK-0521-SIL5 $275
HAISIL 100 2.1mm I.D. PEEK/Titanium CartridgesHAISIL 100 C8 5µm
20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgHK-0221-M085 $180 HK-0421-M085 $270 HK-0521-M085 $275
HAISIL 100 C18 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgHK-0221-M185 $180 HK-0421-M185 $270 HK-0521-M185 $275
59
HA I P E EK P E EK /T I TAN IUM CARTR IDGE COLUMNS
HAISIL 300 2.1mm I.D. PEEK/Titanium CartridgesHAISIL 300 C18 5µm
20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgHK-0221-W185 $190 HK-0421-W185 $310 HK-0521-W185 $315
HAISIL HL 2.1mm I.D. PEEK/Titanium CartridgesHAISIL HL C18 5µm
20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgLK-0221-C185 $190 LK-0421-C185 $300 LK-0521-C185 $310
HEAVY 2.1mm I.D. PEEK/Titanium CartridgesHEAVY C18 5µm
20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgVK-0221-C185 $190 VK-0421-C185 $310 VK-0521-C185 $315
PHALANX 2.1mm I.D. PEEK/Titanium CartridgesPHALANX C18 5µm
20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgPK-0221-C185 $190 PK-0421-C185 $310 PK-0521-C185 $315
PROTO 200 2.1mm I.D. PEEK/Titanium CartridgesPROTO 200 C4 5µm
20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgRK-0221-D045 $190 RK-0421-D045 $310 RK-0521-D045 $315
PROTO 200 C18 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgRK-0221-D185 $190 RK-0421-D185 $310 RK-0521-D185 $315
PROTO 300 2.1mm I.D. PEEK/Titanium CartridgesPROTO 300 C4 5µm
20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgRK-0221-W045 $190 RK-0421-W045 $310 RK-0521-W045 $315
PROTO 300 C18 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgRK-0221-W185 $190 RK-0421-W185 $310 RK-0521-W185 $315
TARGA 2.1mm I.D. PEEK/Titanium CartridgesTARGA C8 5µm
20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgTK-0221-C085 $180 TK-0421-C085 $270 TK-0521-C085 $280
TARGA C18 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgTK-0221-C185 $180 TK-0421-C185 $270 TK-0521-C185 $280
See pages 66 - 67 for information about the Cartridge Holders required for these Cartridges
200
CLIPEUS 10mm I.D. Semi-Prep Cartridges CLIPEUS C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges
30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgCF-0310-C181 $275 CF-0510-C181 $215 CF-1010-C181 $270
CLIPEUS C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgCF-0510-C185 $315 CF-1010-C185 $395
HAISIL 100 10mm I.D. Semi-Prep Cartridges HAISIL 300 C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges
30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgHF-0310-W181 $275 HF-0510-W181 $215 HF-1010-W181 $270
HAISIL 300 10mm I.D. Semi-Prep Cartridges HAISIL 300 C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges
50x10mm 1/pkg 100x10mm 1/pkgHF-0510-W185 $315 HF-1010-W185 $395
PROTO 200 10mm I.D. Semi-Prep Cartridges PROTO 200 C4 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges
30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgRF-0310-D041 $275 RF-0510-D041 $215 RF-1010-D041 $270
PROTO 200 C4 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgRF-0510-D045 $315 RF-1010-D045 $395
PROTO 200 C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges
30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgRF-0310-D181 $275 RF-0510-D181 $215 RF-1010-D181 $270
PROTO 200 C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgRF-0510-D185 $315 RF-1010-D185 $395
60
HAISpeed10 - A high performance10mm I.D. Cartridge SystemHigh throughput requirements forcombinatorial chemistry applicationsoften call for 10mm I.D. columns. HigginsAnalytical has developed a convenientcartridge system that offers an econom-ical solution for demanding combinatorialand semi-preprative needs. Even thougha HAISpeed10 cartridge costs a fractionof conventional 10mm HPLC column ofthe same length, they have the sameconsistently high performance of otherHiggins Analytical columns.
Each 5cm and 10cm cartridge column istested and is shipped with a CofA.
HAISpeed10 CARTRIDGES10mm I.D. Semi-Prep Cartridge Columns
Guide to HAISpeed 10 Cartridge Part NumbersxF-0310-xxxx 30 x 10mm cartridgexF-0510-xxxx 50 x 10mm cartridgexF-1010-xxxx 100 x 10mm cartridgeSee Page 23 for complete Part Number information
HA I S P E ED10 CARTR IDGE COLUMNS AND GUARDS
200
61
HA I S P E ED10 CARTR IDGE COLUMN ORDER ING INFORMAT ION
PROTO 300 10mm I.D. Semi-Prep Cartridges PROTO 300 C4 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges
30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgRF-0310-W041 $275 RF-0510-W041 $215 RF-1010-W041 $270
PROTO 300 C4 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgRF-0510-W045 $315 RF-1010-W045 $395
PROTO 300 C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges
30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgRF-0310-W181 $275 RF-0510-W181 $215 RF-1010-W181 $270
PROTO 300 C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgRF-0510-W185 $315 RF-1010-W185 $395
PHALANX 10mm I.D. Semi-Prep Cartridges PHALANX C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges
30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgPF-0310-C181 $275 PF-0510-C181 $215 PF-1010-C181 $270
PHALANX C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgPF-0510-C185 $315 PF-1010-C185 $395
TARGA 10mm I.D. Semi-Prep Cartridges TARGA C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges
30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgTF-0310-C181 $275 TF-0510-C181 $215 TF-1010-C181 $270
TARGA C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgTF-0510-C185 $315 TF-1010-C185 $395
See pages 66 - 67 for information about Cartridge Holders required for these Cartridges
NEW
10mm guard Cartridges
62
Analytical Guard CartridgesThe complete range of sorbentsmanufactured by Higgins Analytical isavailable in 2cm x 2.1 or 3.2mm I.D.guard cartridges. Not only does thisprovide a wide selection of products forprotecting analytical and narrowboreHPLC columns, it also facilitates a widevariety of trace enrichment, columnswitching, and ballistic gradient applica-tions.
These 2cm long cartridges require areusable holder and are compatible withHiggins Analytical’s HAIPEEK GuardHolders as well as PE/Brownlee’sNewGuard Holder.
HAIPEEK & HAICART GuardsPEEK/TITANIUM and s.s. Guard Cartridges
Guide to HAIPEEK and HAICART Guard Cartridge Part NumbersxK-0221-xxxx 20 x 2.1mm PEEK/titanium guard cartridgexF-0232-xxxx 20 x 3.2mm stainless steel guard cartridgeSee Page 23 for complete Part Number informationNote: Old HAICART P/Ns such as H118, T118, C1PH, etc. can still be used when ordering.
GUARD CARTR IDGES
CLIPEUS Guard CartridgesCLIPEUS C8 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgCK-0221-C085 $180 CF-0232-C085 $180
CLIPEUS C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgCK-0221-C185 $180 CF-0232-C185 $180
CLIPEUS Cyano 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgCK-0221-CNP5 $190 CF-0232-CNP5 $185
CLIPEUS Phenyl 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgCK-0221-PHN5 $190 CF-0232-PHN5 $185
CLIPEUS Silica 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgCK-0221-SIL5 $180 CF-0232-SIL5 $180
HAISIL 100 Guard CartridgesHAISIL 100 C8 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgHK-0221-M085 $180 HF-0232-M085 $180
HAISIL 100 C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgHK-0221-M185 $180 HF-0232-M185 $180
63
GUARD CARTR IDGE ORDER ING INFORMAT ION
HAISIL 300 Guard CartridgesHAISIL 300 C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgHK-0221-W185 $190 HF-0232-W185 $190
HAISIL HL Guard CartridgesHAISIL HL C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgLK-0221-C185 $190 LF-0232-C185 $190
HEAVY Guard CartridgesHEAVY C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgVK-0221-C185 $190 VF-0232-C185 $190
PHALANX Guard CartridgesPHALANX C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgPK-0221-C185 $190 PF-0232-C185 $190
PROTO 200 Guard CartridgesPROTO 200 C4 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgRK-0221-D045 $190 RF-0232-D045 $190
PROTO 200 C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgRK-0221-D185 $190 RF-0232-D185 $190
PROTO 300 Guard CartridgesPROTO 300 C4 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgRK-0221-W045 $190 RF-0232-W045 $190
PROTO 300 C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 0X3.2mm 5/pkgRK-0221-W185 $190 RF-0232-W185 $190
TARGA Guard CartridgesTARGA C8 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgTK-0221-C085 $180 TF-0232-C085 $180
TARGA C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel
20X2.1mm 5/pkg 20X3.2mm 5/pkgTK-0221-C185 $180 TF-0232-C185 $180
64
HA I S P E ED 2 0 - PREP GUARD CARTR IDGES
HAISpeed20 guard cartridges pro-vide unique Protection for 20mmI.D. prep columns.“Prep is a dirty business,” and expensivecolumns can be quickly destroyed bycrude samples if proper precautions arenot taken. Higgins Analytical’sHAISpeed20 Prep Guard Systemprovides effective column protectionwithout compromising performance. Inaddition to an efficiently packed bedwhich retains strongly absorbing junkthat would change the selectivity of theprep column, these unique guardcartridges have 0.5µm frits that removeparticulate contaminants as well.
HAISpeed20 CARTRIDGES20mm I.D. Prep Guard Cartridge Columns
Guide to HAISpeed 20 Cartridge Part NumbersxF-0220-xxxx 25 x 20mm cartridgeSee Page 23 for complete Part Number information
Typical 250x20mm HAISIL 100 C18 5µmTest Chromatogram.
The same 250x20mm 5µm column shown in the top chromatogram retested with a HAISPEED 20 Guard Cartridge in line.
HAISpeed20 guard cartridges provideconvenience and performance
65
HA I S P E ED 2 0 ORDER ING INFORMAT ION
HAISIL 100 20mm I.D. Prep Guard Cartridges HAISIL 100 C18 10µm 20mm I.D. s.s. Prep Guard Cartridges
25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkHF-0220-M181 $295 HF-0320-M181 $150 HF-0520-M181 $200
CLIPEUS 20mm I.D. Prep Guard Cartridges CLIPEUS C18 10µm 20mm I.D. s.s. Prep Guard Cartridges
25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkCF-0220-C181 $295 CF-0320-C181 $150 CF-0520-C181 $200
HAISIL 300 20mm I.D. Prep Guard Cartridges HAISIL 300 C18 10µm 20mm I.D. s.s. Prep Guard Cartridges
25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkHF-0220-W181 $295 HF-0320-W181 $150 HF-0520-W181 $200
PROTO 200 20mm I.D. Prep Guard Cartridges PROTO 200 C4 10µm 20mm I.D. s.s. Prep Guard Cartridges
25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkRF-0220-D041 $295 RF-0320-D041 $150 RF-0520-D041 $200
PROTO 200 C18 10µm 20mm I.D. s.s. Prep Guard Cartridges25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkRF-0220-D181 $295 RF-0320-D181 $150 RF-0520-D181 $200
PROTO 300 20mm I.D. Prep Guard Cartridges PROTO 300 C4 10µm 20mm I.D. s.s. Prep Guard Cartridges
25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkRF-0220-W041 $295 RF-0320-W041 $150 RF-0520-W041 $200
PROTO 300 C18 10µm 20mm I.D. s.s. Prep Guard Cartridges25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkRF-0220-W181 $295 RF-0320-W181 $150 RF-0520-W181 $200
PHALANX 20mm I.D. Prep Guard Cartridges PHALANX C18 10µm 20mm I.D. s.s. Prep Guard Cartridges
25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkPF-0220-C181 $295 PF-0320-C181 $150 PF-0520-C181 $200
TARGA 20mm I.D. Prep Guard Cartridges TARGA C18 10µm 20mm I.D. s.s. Prep Guard Cartridges
25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkTF-0220-C181 $295 TF-0320-C181 $150 TF-0520-C181 $200
Both a Filter and a Guard
The 0.5µm pore size frits onHAISpeed20 cartridges provide uniquebuilt-in filtration for maximum columnprotection. These cartridges are shippedwith end caps that prevent solvent lossand protect the cartridge and frits.
200
NEW
35x20mm and
50x20mm
Even greater capacity
for trapp
ing and
purification
66
No Tools Required for Leak-FreeOperationEven though Higgins Analytical cartridgeholders do not require any tools forassembly and disassembly, their leak-freeoperation assures that maximum per-formance will be achieved in yourdemanding, high-speed separations.
The Holder’s light weight also makes iteasy to handle and assures its compati-bility in challenging plumbing situations.
Cartridge HoldersHolders for 2.1, 3.2, 4.6,10, and 20mm I.D. Cartridges
Guide to Cartridge Holder Part NumbersHK-GUARD-FF Female/Female Holder for 2cm cartridgeHK-GUARD-FM Female/Male Direct Connect Holder for 2cm cartridgeHK-HOLD-40 Holder for 4cm x 2.1, 3.2, 4.6mm cartridgeHK-HOLD-50 Holder for 5cm x 2.1, 3.2, 4.6mm cartridgeHK-HOLD-100 Holder for 10cm x 2.1, 3.2, 4.6mm cartridge10-HOLD-30 Holder for 30x10mm cartridge10-HOLD-50 Holder for 50x10mm cartridge10-HOLD-100 Holder for 100x10mm cartridge20-HOLD-25 Holder for 20 x 25mm cartridge
CARTR IDGE HOLDERS
Higgins Analytical Cartridges and Holders enable a wide range of applications and techniques
Direct-Connect Guards
Loop-Column applications
CARTR IDGE HOLDER ORDER ING INFORMAT ION
67
2cm Guard Cartridge HoldersDirect Connect Holders
Female/Male Standard Female/Male for Wateers ColumnHK-GUARD-FM $160 WK-GUARD-FM $160
Symmetrical Connection HoldersFemale/Female HolderHK-GUARD-FF $175
Holders for Analytical Cartridge ColumnsAnalytical Cartridge Holders
2cm Cartridges 4cm Cartridges 5cm Cartridges 10cm CartridgesHK-HOLD-20 $175 HK-HOLD-40 $175 HK-HOLD-50 $180 HK-HOLD-100 $185
Note: HK-HOLD-20 is the same as HK-GUARD-FF
Holders for 10mm I.D. Semi-Prep Guards and CartridgesHAISPEED 10 Cartridge Holders
3cm Cartridges 5cm Cartridges 10cm Cartridges10-HOLD-30 $175 10-HOLD-50 $180 10-HOLD-100 $185
Holders for 20mm I.D. Prep Guard CartridgesHAISPEED 20 Guard Cartridge Holders
20x25mm Cartridges 20x35mm Cartridges 20x50mm Cartridges20-HOLD-25 $270 20-HOLD-35 $270 20-HOLD-50 $270
Higgins Analytical manufacturers the widest range of guard cartridges in the world
Analytical (2.1 and 3.2mm)
Semi-Prep (10mm)
Prep Scale (20mm)
NEW
20x35 & 20x50mm Holders
See page 63 for the New Cartriges
68
HA I F I LTER - H IGH P ERFORMANCE IN - L INE F I LTER - NO TOOLS REQU IRED
A simple, yet effective, means ofprotection is to place a filter in theflow path between the sampleinjection valve and the HPLC col umn.While this technique is commonlyused, it is also com monly plagued byleaky holders and significantperformance loss. TheHiggins Analytical HAIFILTER resolvesall of these problems!
Simplicity and ConvenienceThe Higgins Analytical HAIFILTER pro videsefficient and leak-free in-linefiltration, yet it does notrequire any tools. To make iteven more convenient, theHAIFILTER is designed toconnect directly to the inletfitting on the HPLC column,maximizing per- formance, and minimizinghardware. The 0.5µm porosity frits willremove much of the particulate contami -nation that is largely responsible for col umnpressure build up and plugging. The holder’sinert construction, and the avail ability ofPEEK and titanium as well as stainless steelfrits, insures that the HAIFILTER can addressall of your in-line filtration needs.
Two Convenient FormatsHAIFILTER is now available in two for mats.The leak-free, finger-tight, and effi cientperformance are qualities of the HAIFILTERthat have made it a popular product,replacing the competition’s awk ward andleaky alternatives in hundreds oflaboratories worldwide. The direct -connect feature in the original designmakes the HAIFILTER FM ideal for pro -tecting HPLC columns while the symmet -rical female thread fittings on each end ofHAIFILTER FF provide unequaled flexibil ityto address your HPLC system’s filtra tionrequirements.
KEY FEATURES:
• Low cost, yet effective column protection
• Direct column connection and symmetrical versions
• No tools required • Leak free operation
• 0.5µm filters in stainless steel, titanium or PEEK
• Inert construction’s threads won’t oxidize or gall
PerformanceTry the experiment summarized by thechromatograms above with another man -ufacturer’s in-line filter. You may be verysurprised how much more other in linefilters contribute to peak broadeningwhen comparison to the miniscule 28µL2
at 200µL/min volume-variance that is
HPLC Columns without a HAIFILTER
HPLC Columns with a HAIFILTER
N=24,664
N=19,525
N=24,336N=17,708
250x4.6mm (P/N LS-2546-C185)
250x4.6mm (P/N LS-2546-C185)
250x2.1mm (P/N HS-2546-M185)
250x2.1mm (P/N HS-2546-M185)
HAIFILTER “We found the HAIFILTER to be an ideal solution for our high performance
in-line filtration needs, and it doesn’t require any tools or wrenches!”
obtained with the HAIFILTER. Thisexcep tional efficiency is confirmed bychroma tographers reporting that using aHAIFILTER permits the routine analysis ofsolid-phase sample extracts by LC/MSwith good efficiency and greatlyenhanced HPLC column life.
REINTRODUCED!
Even tho
ugh HAIF
ILTER wa
s
removed
from our
catalog s
everal
years ago
, it conti
nues to b
e so
popular w
ith our o
ld custom
ers,
we’ve de
cided to
include
it again.
Best par
t?
Price is u
nchanged
!
HA I F I LTER & PARTS AND ACCES SOR I E S ORDER ING INFORMAT ION
69
PARTS AND ACCES SOR I E S
Micro Column Connector KitKit facilitates plumbing a Piccolo, Sprite, Capellini 6-32, or a Caπllary to your instrument
MIC-KIT $100Kit Contains:
6-32 PEEK Bushings (4 each)10-32 PEEK Long Bushings (2 each)Green Sleeve 0.155 x 0.025” (4 each)Brown Sleeve 0.062 x 0.015” (2 each)Fused Silica Capillaries 10cm x 150µm (4 each)
HAIFILTER FM (Female/Male) Starter KitsHF-FKIT HAIFILTER FM (Standard) $270HF-WKIT HAIFILTER FM (for Waters HPLC columns) $270HF-RKIT HAIFILTER FM (for Rheodyne injection valves - $270
Specify valve model when ordering)
Note: HAIFILTER FM Starter Kits include a package of 10 filters (HF-SFILT), a 10cm piece ofPEEK tubing, and a Delrin bushing and a PEEK ferrule.
HAIFILTER FF (Female/Female) Starter KitFF-FKIT HAIFILTER FF $270
Note: The HAIFILTER FF Starter Kit includes a package of 10 filters (HF-SFILT), two 10cmpieces of PEEK tubing, and two Delrin bushings and two PEEK ferrules.
Replacement FiltersAll replacement kits contain 10 filters per packageHF-SFILT Stainless steel HAIFILTER replacement filters pkg of 10 $85HF-TFILT Titanium HAIFILTER replacements filters pkg of 10 $140HF-PFILT PEEK HAIFILTER replacements filters pkg of 10 $160
HAIFILTER Holders and Replacement Filters
FlexibilityNot only is the HAIFILTER available intwo formats, it can also be directlyconnected to a sample injection orswitching valve. The photograph to theright demon strates how the HAIFILTERhas been designed to clear the bushingson a popular Rheodyne sample injectionvalve.
FILTER replacement is easy and requires no tools
TECHN ICAL SUPPORT AND ORDER ING INFORMAT ION
Expert and Responsive TechnicalSupport As a Higgins Analytical Customer seekingtechnical support or product information,you will eliminate long waits andexpensive phone calls, and you will neverbe transferred from one person toanother only to end up in someone’sphone mail! By simply faxing (1-650-988-8931) or e-mailing ([email protected])your question to our Technical SupportGroup, you will receive a prompt writtenresponse. If we don’t know the answer,we will tell you so – but, that doesn’thappen very often. Additional up-to-dateproduct and application information canbe found by pointing your browser at ourweb site. www.higanalyt.com
Easy Ordering and Rapid Delivery Our worldwide network of qualifieddistributors can provide expert supportand prompt delivery from their localinventory. Our catalog-listed items aregenerally shipped from the factory withina day of receiving the order. In the eventthat we have not provided you with anear by distributor, we can ship an orderto you directly from the factory by avariety of carriers.
In order to further simplify the process foryou and your purchasing department,
Higgins Analytical and many of its distribu-tors accept credit card orders. Paymentterms for all other orders are net 30 daysfrom date of invoice.
Product Availability Higgins Analytical products are availableworldwide through a network of respon-sive distributors. These organizations canprovide qualified technical support andprompt product delivery. The names canbe found by sending us a fax (650-988-8931) or by checking our web site which,in addition to names, addresses and phonenumbers, contains many hyperlinked e-mail and URL addresses.
70
WARRANTY, PATENTS AND TRADEMARKS
HIGGINS ANALYTICAL PRODUCTSMAY BE PURCHASED AND ARE SOLDONLY ON THE FOLLOWING WARRAN-TY TERMS AND CONDITIONS. ADDI-TIONAL OR DIFFERENT TERMS,WHETHER IN AN ORDER, ACKNOWL-EDGMENT, INVOICE OR OTHER DOCU-MENT, SHALL NOT BECOME PART OFOR VARY THE FOLLOWING TERMSUNLESS AGREED TO IN WRITING BYHIGGINS ANALYTICAL.
LIMITED WARRANTY
Higgins Analytical products are manufac-tured and quality controlled to the highestindustry standards, and Higgins Analyticalwarrants that the products, when properlyapplied in accordance with HigginsAnalytical's standards therefor, will performfor a period of thirty (30) days after deliverysubstantially as described in HigginsAnalytical's then current specifications forthe product. If a customer believes a prod-uct does not comply with such warranty, thecustomer shall, within the warranty period,report the purported defect to HigginsAnalytical and cooperate fully with HigginsAnalytical in determining the conditions andcause of any purported defect and, thereby,obtain a returned goods authorization fromHiggins Analytical or the place of purchase.If Higgins Analytical determines the allegeddefect occurred as a result of misuse, neg-lect, improper installation, repair, alteration,application or accident, rapid depressuriza-tion, removal of column end fittings, unusualphysical or electrical stress or improper han-dling procedure, the warranty shall be voidand ineffective.
EXPRESS LIMITATIONS
HIGGINS ANALYTICAL PRODUCTS AREMANUFACTURED FOR USE INRESEARCH IN CHEMICAL MANUFAC-TURING LABORATORIES ONLY ANDARE NOT INTENDED FOR USE INCLINICAL OR DIAGNOSTIC APPLICA-TIONS. THE HAISPEED10 AND HAI-SPEED20 CARTRIDGE HOLDERS ARERATED FOR USE UP TO 3,000 PSI(200BAR) WITH FLUIDS THAT ARE LIQ-UID AT STP (STANDARD TEMPERATUREAND PRESSURE) ONLY. SERIOUS BODI-LY INJURY OR DEATH MAY RESULT IF AHAISPEED HOLDER BROKE WHILEWORKING WITH SUPERCRITICAL FLU-IDS.
DISCLAIMER
EXCEPT AS EXPRESSLY PROVIDED, ALLWARRANTIES, CONDITIONS, REPRE-SENTATIONS, INDEMNITIES ANDGUARANTEES, WHETHER EXPRESS ORIMPLIED, ARISING BY LAW, CUSTOM,PRIOR ORAL OR WRITTEN STATE-MENTS BY HIGGINS ANALYTICAL OROTHERWISE (INCLUDING, BUT NOTLIMITED TO, ANY WARRANTY FORMERCHANTABILITY OR FITNESS FOR APARTICULAR PURPOSE) ARE OVERRID-DEN, EXCLUDED AND DISCLAIMED.
EXCLUSIVE REMEDY
In the event a customer establishes abreach of the above warranty, customer'sexclusive remedy against Higgins Analytical,and Higgins Analytical's sole liability, is, at itsoption, the replacement of the defectiveproduct or the refund of the purchase pricetherefor.
NO CONSEQUENTIAL DAMAGES
HIGGINS ANALYTICAL SHALL NOT BERESPONSIBLE FOR ANY CONSEQUEN-TIAL, INDIRECT, SPECIAL, PUNITIVE ORINCIDENTAL DAMAGES, WHETHERFORESEEABLE OR UNFORESEEABLE,INCLUDING, BUT NOT LIMITED TO,CLAIMS FOR LOSS OF GOODWILL,PROFITS, USE OF MONEY OR USE OFPRODUCTS, STOPPAGE OF OTHERWORK OR IMPAIRMENT OF OTHERASSETS, ARISING OUT OF BREACH OFEXPRESS OR IMPLIED WARRANTY,BREACH OF CONTRACT, MISREPRESEN-TATION, NEGLIGENCE, STRICT LIABILITYIN TORT OR OTHERWISE, EXCEPTONLY IN THE CASE OF PERSONALINJURY WHERE AND TO THE EXTENTTHAT APPLICABLE LAW REQUIRESSUCH LIABILITY.
PATENTS AND TRADEMARKS
Kromasil is a trademark of Eka Nobel.NewGuard and MPLC are trademarks ofPerkin-Elmer. PICOTAG is a trademark ofWaters. CAPELLINI, CLIPEUS, HAISIL,HAICART, HAIFILTER, HAIPEEK, HAISPEED, HEAVY, TARGA®, PHALANX,PROTO, HAIGUARD, PICCOLO, SPRITE,and Caπllary are trademarks of HigginsAnalytical, Inc.
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Higgins Analytical, Inc.1235 Pear Avenue, Suite 105Mountain View, California, 94043 USAPhone: 650-988-8930 or 800-988-6889Fax: [email protected] www.higanalyt.com
Expert Manufacturers of HPLC Consumables
Higgins Analytical develops and manufactures a wide range of stainlesssteel and PEEK (polyetheretherketone)High Performance Liquid Chromatography(HPLC) columns and cartridges. Theseunique offerings address many needs ofchromatographers desiring fast analysistimes, efficient system and columnprotection, and chemical inertness.
Special application areas include LC/MS,combinatorial chemistry, column switchingtechniques, in-line filtration, and guardcolumn requirements. Products aresupplied and supported worldwide by a network of responsive distributors.
We are a quality-oriented chromatographycompany dedicated to providingperformance products, expert support,and rapid delivery to research and quality control laboratories worldwide.
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