HAI Price List 2005 - HPLC Column Manufacturer

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Higgins Analytical EXPERT MANUFACTURER OF HPLC CONSUMABLES 2013

Transcript of HAI Price List 2005 - HPLC Column Manufacturer

Page 1: HAI Price List 2005 - HPLC Column Manufacturer

HigginsAnalytical

EXPERT MANUFACTURER OF HPLC CONSUMABLE S

2013

Page 2: HAI Price List 2005 - HPLC Column Manufacturer

WORLDWIDE AVA I LAB I L I TY AND SUPPORT

Higgins Analytical ist eine Qualitäts orientierte Firma,welche sich der Lieferung von leistungsfähigenProdukten widmet, Kundenunterstützung durchExperten, sowie rasche Lieferung an Forschungs undQualitätskontrolleinrichtungen ist weltweit unser Ziel.

La Higgins Analytical é una societá orientata versol’alta qualitá, dedicata a fornire ai laboratori diricerca e controllo qualitá nel mondo prodotti adalto livello, supporto di esperti e rapide consegne.

Higgins Analytical is a quality oriented company, dedicatedto providing high performance products, expert support, andrapid delivery to research and quality control laboratoriesworldwide.

Higgins Analytical es una compañia dedicada a poveerproductos de óptima calidad, excelente rendimiento,experto soporte técnico y entrega immediata a los lab-oratorios de investigación y de control de calidad delmundo entero.

I Higgins Analytical iyinkampani engusaziwayo kwe-zobuchwepheshe. Izinikele ekuthini ikhiqizeumkhiqizo oseqophelweni eliphezulu. Izebenzisauxhaso oluphambili ukukhipha imiphumela ecut-shungwe kahle nangokushesha kanye nezindawozokucubungula eziseqophelweni eliphezulu emh-labeni jikelele.

Higgins Analytical est une société ayant pourobjectif la qualité et de proposer des produitsperformants, un support expert, des livraisonsrapides pour les laboratories de recherches etde contrôle qualité à travers le monde.

Higgins Analities is ‘n kwaliteits-georienteerdemaatskappy wat toegewyd is om produkte vanhoogstaande gehalte, kundige ondersteuning ensnelle lewering van produkte vir navorsings-engehalte-beheer laboratoria wereldwyd te voorsien.

Higgins Analytical on kromatografiakolonneja valmistava yritys, joka tarjoaa laadukkaita tuotteita, asiantuntevaapalvelua ja nopeat toimitukset tutkimus- ja laadunvalvon-talaoratoriolle maailmanlaajuisesti.

Higgins Analytical, hedefi yüksek performanslı ürünlersağlamak olan, dünya genelinde araştırma ve kalitekontrol labratuvarlarına expert destek veren ve hızlıdaḡıtım yapan kalite odaklı bir şirkettir.

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HIGGINS ANALYTICAL 2 - 3

CLIPEUS™ 4 - 5

HAISIL 100™ 6 - 7

HAISIL 300™ 8 - 9

HAISIL HL™ 10 - 11

HEAVY™ 12

Some Interesting Facts 13

PROTO™ 200 14 - 15

PROTO™ 300 16 - 17

PHALANX™ 18 - 19

TARGA® 20 - 21

Specialty Columns 22

Par t Number Guide 23

HPLC COLUMNS 24 - 29

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CONTENTS

MICROBORE COLUMNS 30 - 35

SEMI-PREP Columns 36 - 39

Caπllary™ NEW 40 - 41

Capellini™ 6-32 42 - 49

SPRITE™ 50 - 51

PICCOLO™ 52 - 53

s.s. Analytical Car tridges 54 - 57

PEEK/Titanium Car tridges 58 - 59

10mm Car tridge Columns 60 - 61

Analytical Guard Car tridges 62 - 63

Prep Guard Car tridges 64 - 65

Car tridge Holders 66 - 67

H HAIFILTER NEW 68 - 69XX-XX-XXX XX-XX-XXXX XX-XXXX-X

π 75µm and 150µm Columns

and PREP Columns

Ordering, Warranty, Trademarks 70 - 71

NEW 30x1.0mm for more capcity

NEW 35x20 & 50x20mm sizes

and Services

Par ts and Accessories

Capellini™ 10-32 NEW

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H IGG INS ANALYT ICAL

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Experience, Expertise, ContinuityHiggins Analytical was founded in early1994, but that is only half of the story.The two founders had already beenpacking commercial HPLC columns formore than fifteen years before that.Besides a lot of expertise, our experi-ence brings an unrivaled continuity tothe field.

Total Quality Approach to allAspects of Design, Manufacturingand Testing Each HPLC column and analyticalcartridge from Higgins Analytical isevaluated by a computerized perform-ance test prior to receiving a quality

control approval. This testing protocolinvolves a highly accurate statisticalmoments determination of the column’sperformance characteristics. Thesesensitive peak shape and columnefficiency measurements can only bemade by computerized data loggingmethods. The raw digital chromatogramfor every column we have manufacturedsince March 1994 is retrievable from ourarchives.

Product TraceabilityThe test data for these serializedcolumns include sorbent properties andlot number as well as complete descrip-tion of the test conditions. We are ableto trace the serial number of every

column to the customer that originallypurchased the column.

Comprehensive Record Keepingand Statistical Quality ControlRaw digital test data is automaticallyarchived for every column and analyticalcartridge we manufacture. As a result, itis a simple matter for us to retrievecomprehensive quality control informa-tion and routinely apply it to ourstatistical process control activities. This

commitment to quality manufacturingand comprehensive record keepingpermits us to produce replacementCertificates of Analysis when requestedby laboratories complying with theirinternal GLP needs. And, most

importantly, we are able to assistcustomers during the lengthy processbeginning with method development andoptimization to validation and NDA filingby providing comprehensive serialnumber, manufacturing date, sorbent lot,and performance data for every columnthey ever purchased.

Expert Manufacturing and State-of-the-Art Equipment Two of Higgins Analytical’s staff havebeen involved with the chemical separa-tions business for over 30 years, othersfor only 10 or 15. By focusing thisexpertise exclusively on the separationsconsumables business, high qualityproducts as well as high quality processesare assured. A key element of thisexpertise is the electronic manufacturing

lab book that we have developed. All ofour column packing equipment iscontrolled by this electronic book, and allmethods are electronically stored. It isimpossible to pack a column without theelectronic lab book being completelydocumented. Processes cannot be

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Quality and Environmental Aspectsof Process SolventsProper composition of slurry and pack-ing solvents is vital to the whole process.Our solvent purification equipment andprocedures allow us to address all of thesolvent requirements for production andquality control. Not only does thisinvestment permit us to use the highestpurity solvents available, it also eliminatesmuch of the expensive and environment-polluting solvent disposal practice char-acteristically found in today’s chemicalindustry.

Unique Bonded Phase SynthesisChemists at Higgins Analytical have beenproducing bonded phases since 1979.During this long experience, we havepioneered many technologies longbefore they were “discovered” by otherswith a bigger marketing clout. HigginsAnalytical’s introduction of a “No

Requirement for TFA” HPLC column inearly 1997 is just one example of ourvanguard approach to this field.

Computer Aided Drawings andNumerical Control MachineryKey to reinforcing our ability to providethe highest performance-to-price ratioproducts available in the separationsconsumables industry has been ourinvestment in computer aided design(CAD) technology and computernumerically controlled (CNC) machinery.SolidWorks™ software is used by ourengineers which permits us to designHPLC hardware and see it in its

PROCES S AND PRODUCT CONTROL

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changed without an expert supervisor’sintervention. When new processes fornew products are developed oroptimization is required for an existingmethod, a complete audit trail is createdfor every condition change that is made.The packing pumps operate with PID(proportional integral/derivative)controllers allowing for control flow rate,

rather than pressure, throughout thepacking process. This is a very importantpoint. Common folklore is that it takeshigh pressure to pack an HPLC column.Actually, pressure is only the result of a

complex two phase (liquid/solid) flow.This flow, not pressure, is what producesa packed bed. The “pressures” used bymany column manufacturers are theresult of flow rates that approach thevelocities that will produce a “fluidizedbed” during certain phases of the packingprocess, not exactly a condition that willproduce a stable, optimally packedcolumn. In addition to being able tocontrol our equipment digitally, we areable to data log the actual conditions aswell.

Our unique and highly automatedcolumn packing process is based on aclosed, continuous flow system employingrecoverable, unreactive, and safe fluids.And the best part about it is that itenables Higgins Analytical to manufacturea wide variety of HPLC columns withunrivaled efficiency.

assembled 3D form before we manufac-ture it. Dedicated tooling and securebackups of machine code assure fastsetups and consistant high qualityproduction of all the stainless steel andplastic components in each product line.

Our product development, manufac-turing, and testing processes strictlyfollow a systematic and welldocumented cycle.

A Certificate of Analysis, like the one below for a3cm x 10mm cartridge, accompanies each

HPLC Column and Cartridge we manufacture

Note: This high performance cartridgeis typically used as a guard column.

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CL I P EUS

CLIPEUS A “Small Shield” for a Big Job Phases Silica, C8, C18, Cyano, Phenyl

Particle Sizes 4.5 and 10µmPore Size 120Å

Pore Volume 0.8mL/gmSurface Area 330m2/gm

Carbon%(w/w) C18 = 18%Phase type Extremely well end-capped

monofunctional phasesSilica Class Type B

ApplicationsThe high purity base silica and thoroughlyend capped bonded phases insure thatCLIPEUS columns and cartridges will per-form well in demanding situations wheremany other column brands fail. Evenpolar compounds elute as sharp and sym-metrical peaks permitting surprisinglyshort columns to be used for high speedanalysis. In addition to Silica, C8 and C18phases, CLIPEUS columns are availablewith phenyl or cyano functionality.

Methylxanthines

CLIPEUS C18 150x4.6mm P/N CS-1546-C185

25% MeOH/water (unbuffered)1mL/min, 30ºC, 254nm

1. Theobromine 2. Theophylline3. Caffeine 4. Phenol

Carbamates and Urea PesticidesEPA 632A

Industrial and Municipal Waste WaterColumn P/N CS-2546-C185

1. Monuron 2. Propoxur 3. Carbaryl 4. Propham5. Diuron 6. Linuron 7. Methiocarb 8. Mexacarbate 9. Chlorpropham 10. Barban 11. Neburon

This unbuffered Pyridine and Phenol chromatogram illus-trates the absence of any undesirable solute/silanol inter-action on a CLIPEUS C18 HPLC Column.

CLIPEUS C18 5µm 150x4.6mm

40% MeOH/water (unbuffered)1mL/min, 25ºC, 254nmP/N CS-1546-C185

Sunscreen Formulation

CLIPEUS C18 250x4.6mm P/N CS-2546-C185

87.5% MeOH/ 12.5% water (unbuffered)1mL/min, 30ºC, 285nm

1. Oxybenzone 2. Padimate O3. Octyl Methoxycinnamate

Guide to CLIPEUS Part NumbersCx-xxxx-SIL5 CLIPEUS Silica 5µmCx-xxxx-C085 CLIPEUS C8 5µmCx-xxxx-C185 CLIPEUS C18 5µmCx-xxxx-CNP5 CLIPEUS Cyano 5µmCx-xxxx-PHN5 CLIPEUS Phenyl 5µmSee Page 23 for complete Part Number information

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Comparative Selectivity of CLIPEUS HPLC Columns

Neutrals Test Mix NIST Test Mix

1. Uracil 2. Toluene 3. Ethylbenzene4. Quinizarin 5. Amitriptyline

1. Thiourea 2. Phenol 3. Acetophenone4. Nitrobenzene 5. Toluene 6. Napthalene

CLIPEUS C18 150x4.6mm P/N CS-1546-C185

CLIPEUS C8 150x4.6mm P/N CS-1546-C085

CLIPEUS Phenyl 150x4.6mm P/N CS-1546-PHN5

CLIPEUS Cyano 150x4.6mm P/N CS-1546-CNP5

50% MeCN/water, 1.5mL/min, 205nm 80% MeOH/Buffer, 1.5mL/min, 254nmBuffer: 20mM potassium phosphate

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CL I P EUS APPL ICAT IONS

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HA I S I L 1 0 0

HAISIL 100 The WorkhorsePhases Silica, C8, and C18

Particle Sizes 3µm and 5µmPore Size 100Å

Pore Volume 0.5mL/gmSurface Area 190m2/gm

Carbon%(w/w) C8 = 7%(w/w), C18 = 12%(w/w)Phase type Monofunctional & fully endcappedSilica Class Type A

ApplicationsHAISIL 100 columns and cartridges arevery good general purpose columns thatare particularly well suited for small mol-ecules and peptide applications. TheHAISIL 100 column and cartridge effi-ciencies are unrivaled yet they representone of the most economical productlines in the market today. In formatsranging from 0.075 to 20mm ID andfrom 10 to 250mm in length,

100Å vs. 300Å Columns for PeptideMapping The question of whether to use a 100Åor 300Å column for mapping usuallydivides peptide chromatographers intotwo groups. Rather than take sides, wehave chosen to offer very efficientcolumns with both pore diameters tosuit both camp's preferences. HAISIL100 and HAISIL 300 columns are avail-able in 10 to 250mm lengths and0.075mm to 20mm internal diameters.

Tryptic Map of Transferrin on HAISIL 100250x4.6mm P/N HS-2546-M185

Tryptic Map of Transferrin on HAISIL 300250x2.1mm P/N HS-2521-W185

Guide to HAISIL 100 Part NumbersHx-xxxx-SIL3 HAISIL 100 Silica 3µmHx-xxxx-M183 HAISIL 100 C18 3µmHx-xxxx-SIL5 HAISIL 100 Silica 5µmHx-xxxx-M085 HAISIL 100 C8 5µmHx-xxxx-M185 HAISIL 100 C18 5µmSee Page 23 for complete Part Number information

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Enzymatic Reaction Mixtures of Human 15-lipoxygenase

HAISIL 100 C18 250x4.6mm74.9% MeOH, 25% water, 0.1%HoAC

1mL/min, 234nmP/N HS-2546-M185

Economical Alternative for PICOTAG Analysis

Our HAISIL 100 C18 columns havedemonstrated superior resolution and lifetime for PICOTAG™ amino acid analysiswhen compared to considerably moreexpensive columns from other manufac-turers. Besides good linearity and columnlifetime, the 150x4.6mm column yieldsvery efficient amino acid analysis asshown in the chromatogram above (P/NHS-1546-M185).

ConditionsHAISIL 100 C18 5µm 150x4.6mmSolvent A: Phosphoric acid, ammonium

sulfate aqueous bufferSolvent B: Acetonitrile. Linear gradient at 1mL/min254nm detection

Analogs:A1: pGlu-His-Trp-Ser-Tyr-(d-Ser(but))-Leu-Arg-Pro-NHEtA2: pGlu-His-Trp-Ser-Tyr-d-Ala-Leu-Arg-Pro-NHEtA3: pGlu-His-Trp-Ser-Tyr-d-Leu-Leu-Arg-Pro-NHEtA4: pGlu-His-Trp-Ser-Tyr-d-Ala-Trp-Leu-Pro-NHEtA5: pGlu-His-Trp-Ser-Tyr-d-Trp-Leu-Arg-Pro-NHEt

Hormone Analogs LH-RH (Luteinizing Hormone - ReleasingHormone) Analogs are used for theinhibition of gonadal steroid production.An important application area is in thetreatment of prostate cancer.

Kinetic Measurements in DiseaseResearch Researchers at the University ofCalifornia, Santa Cruz have recentlypublished a novel HPLC method thatyields baseline separation of per-deuter-ated and protonated lipoxygenases.

The striking ability of a 250x4.6mmHAISIL 100 C18 column to resolvethese two very similar compounds isenabling researchers to study largecompetitive kinetic isotope effects inHuman 15-lipoxygenase catalysis.Protio/per-deutero ratio data obtainedby this HPLC method agreed withinexperimental error of data obtained byES-MS.

The elucidation of these mechanisms isnoteworthy because of the increasingrole human lipoxygenases are playing ininflammatory diseases and cancergrowth regulation.

Alkaloids A Peruvian vine named “Una de Gato”(Uncaria tomentosa) and commonlyreferred to as Cat’s Claw, has become apopular natural product source forherbal remedies. Enthusiasts claim theoxindole alkaloid extracts to be effectivein the treatment of a wide range ofdigestive and circulatory diseases as wellas other afflictions ranging from cancerto arthritis. Reputable providers of suchphytochemical containing formulationscarefully assay the alkaloid content of theextracts and formulations. Thechromatogram above illustrates baselineresolution of Cat’s Claw bark extractscontaining isopteropodine, pteropodine,mitraphylline, isomitraphylline,ryncophylline and isorynchophylline.

Alkaloids from Cat’s Clawanalyzed on a HAISIL 100 C18 column

P/N HS-2546-M185

HAISIL 100 - the Separations WorkHorse The 100Å pore size, monomeric bondedphase, and very high performance makeHAISIL 100 an ideal choice for separa-tions ranging from small molecules suchas carbamate pesticides and pharmaceu-

tical drugs to synthetic oligonucleotidesand small peptides. High efficiency,symmetrical peaks, and long column life ischaracteristic not only of the C8 andC18 reverse phase columns, but thenormal phase silica columns as well.

Carbamate Pesticide Analysis250 x 4.6mm HAISIL 100 C18

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HA I S I L 1 0 0 APPL ICAT IONS

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HA I S I L 3 0 0

HAISIL 300 For BiomoleculesPhases C18

Particle Sizes 5 and 10µm*Pore Size 300Å

Pore Volume 0.7mL/gmSurface Area 80m2/gm

%Carbon (w/w) C18 = 6%Phase type Monofunctional and fully endcappedSilica Class Type A

ApplicationsHAISIL 300 columns and cartridges areideal for biomolecule separations such aspeptides and small proteins. Optimizedphase bonding and column packingtechniques assure unsurpassed perform-ance and column-to-columnreproducibility. Short cartridge columnsare economical and are ideally suited forLC/MS applications. The column andcartridge offering ranges from 150µm to20mm I.D. which means that biosepara-tions can be optimized by selecting aformat that best fits your analyticalrequirements.

Micro-Preparative HPLC Higgins Analytical Cartridge Columns arehelping researchers understand theimmune response of living cells. An inves-tigation to understand the genetic con-trol of the immune response systems inorganisms involves the extraction, isola-tion, and identification of more than10,000 peptides. These distinct peptidesare bound to a major histocompatibilitycomplex (MHC) molecule on tumorcells.

Extraction of approximately 5x1010

cellsyield several nanomoles of a very com-plex peptide mixture. Several rounds ofRP-HPLC with different organic modifierson a 40x2.1mm PEEK cartridge column(HAISIL 300 C18 5µ m, P/N: HK-0421-W185) are performed to fractionate themixture into individual components.

The chromatograms on the left illustratehow this technique is being used to nar-row the search for the one peptide thatmarks a particular tumor cell as beingdiseased enabling it to be recognized bythe immune cells.

Fractionation of a Purified Tumor Cell Extract

Peptides ranging from 100 fmol to 1pmol are collected in 40secintervals during a 70min gradient from 0 - 60% MeCN with HFBAmodifier. A 40x2.1mm HAISIL 300 C18 5µm cartridge column(HK-0421-W185) is used.

Reanalysis of a Fraction from the Chromatogram above

The same 40x2.1mm HAISIL 300 cartridge is used for a 70mingradient from 0-60% MeCN with TFA modifier. Tic marks indicate40sec fraction collections. Peek and Titanium cartridge columns

used in the application described above.P/N: HK-0421-W185

Guide to HAISIL 300 Part NumbersHx-xxxx-W185 HAISIL 300 C18 5µmSee Page 23 for complete Part Number information

* 10µm is offered in HAISpeed 20 Guard Cartridges only. See pages 68-69.

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Fast Analysis of a Complex DrugDelivery SystemMost pharmaceutical QC laboratoriesare faced with rapidly increasingdemands for high throughput capability.A good example of one laboratory's

approach to addressing a large numberof samples for analysis on a daily basis issummarized below. This novel drugdelivery system contains a small proteinand other components that span anorder of magnitude in molecular weight.All the compounds of interest wereeasily resolved in a single analysis with afast linear gradient on a 5cm longHAISIL 300 C18 5µm column.

Very High Peak CapacityDepending upon the type of informationa protein chemist is seeking, the HPLCanalysis of a complex digest can take 1to 2 hours. The following chromatogram

illustrates a high resolution tryptic digestmap of BSA on a 250x2.1mm HAISIL300 C18 5µm column indicates peakcapacities in excess of 100. Smallproteins, on the other hand can yieldfewer fragments, and if only a "finger-print" of a digest is required, high peakcapacity columns can be a waste oftime.

HA I S I L 3 0 0 APPL ICAT IONS

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Tryptic Map of BSAon HAISIL 300 C18 5µm 250x4.6mm

P/N HS-2546-W185

Peptide Map in 5 minutesThe figure below illustrates a very fast

peptide map of cytochrome-C. Theinformation that can be gleaned from thisfive minute profile is more than adequatefor many analytical requirements. Therobust 50x4.6mm HAISIL 300 C18column provides hundreds of runswithout performance loss even underthe aggressive 6ml/min flow conditionsused in this example.

The cytochrome-C is a good example ofpushing a column beyond its optimumoperating conditions (more than sixtimes optimum linear velocity) and stillbeing able to get desired results. Thesame column can be run under moreconventional conditions with a flattergradient to optain even higher resolutionseparations. The rhGH tryptic map belowwas done on a 5cm long column thatreplaced the conventional 25cm columnthat was used initially.

The Role of Column Length inGradient Separations Changes in column length have a directand linear effect on theoretical plates andanalysis time in isocratic separations.Column length plays a less important role,however, in gradient techniques. The threechromatograms below illustrate how wellpacked short HPLC columns yield verysimilar peptide separations under identicalgradient conditions.

For this study, the 2.1mm I.D. HAISIL 300C18 5µm columns ranged from 250 to100mm in length. While solvent andanalysis time savings may be minimal,column cost savings can be significantwhen using as short a column as possible.Short columns also exhibit lower pressureat high flow rates.

250x2.1mmHAISIL 300 C18 5µmHS-2521-W185

150x2.1mmHAISIL 300 C18 5µmHS-1521-W185

100x2.1mmHAISIL 300 C18 5µmHS-1021-W185

1. Gly-Tyr2. Val-Tyr-Val3. Met Enkephalin4. Leu Enkephalin5. Angiotensin II

Fast Tryptic Map of rhGH50x4.6mm HAISIL 300 C18

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HA I S I L HL

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HAISIL HL High LoadPhases C18

Particle Sizes 5µmPore Size 100Å

Pore Volume 0.8mL/gmSurface Area 300m2/gm

%Carbon (w/w) C18 = 18%Phase type Monofunctional & fully endcappedSilica Class Type B

ApplicationsHAISIL HL “high load” columns and car-tridges are intended for applicationsrequiring maximum loading and selectivi-ty. The high surface area permits highbonded phase loading yet the monofunc-tional bonded phase and the absence ofmicro-pores eliminates the problems thatplague many of the so-called “high capac-ity” columns currently on the market.The Type B silica backbone is pure,robust, and stable under harsh condi-tions. Users report satisfactory lifetimewhen used for prolong periods at pH ashigh as 9.

High Speed Analysis of ParabenFood PreservativesParabens represent another example offood additives that are routinely analyzedby HPLC. Often, the food matrix beingassayed for preservative content requiresexhaustive cleanup and pre-filtration inorder to prevent clogging of columnswith nonporous or 2µm sorbentsrecently suggested for this analysis. As inthe previous diet cola example, thecompounds of interest are base lineresolved under isocratic conditions. Theinexpensive HAISIL HL 5µm 20x2.1mmcartridge column and the 50 secondanalysis time contribute significantlytowards reducing operating costs.

Fast Paraben Analysis

HAISIL HL C18 5µm20x2.1mm CartridgeLK-0221-C185

700µL/min, 40% MeCN/water

Abstract Previously, we presented a complete 20-minute "fast" Edman chemistry cycle forthe Applied Biosystems ProteinSequencer, HT series (49X-HT). Herewe describe a new "fast" cycle for thehigh sensitivity cLC series (49X-cLC)instrument. The 35-min cycle includes a20-min reversed phase capillary HPLCseparation of the phenylthiohydantoin(PTH)-amino acids. The normal cycletime of the 49x-cLC was approx. 50minutes; therefore approximately 10hours were required to complete 10residues of sequencing. With the newcycle, 10 residues of sequencing can be

completed in only 7.5 hours. Using thisrapid cycle on the 492 or 4-cLC proteinsequencer, 10 residues of sequencing on2 different proteins can be automaticallycompleted within a night.In developing this rapid cycle, we havecreated new cartridge and flask cyclesand sequencing methods. We are using ashorter C18 RP-HPLC capillary columnfor the chromatography and have devel-oped a new gradient. We will describeall the changes made to the standardsystem. We will also present protein andpeptide sequencing results that weregenerated using this rapid cycle.

A new “fast” cycle for high sensitivity Edman Sequencing

Poster 4P-52378th Annual Meeting of he Japanese Biochemical Society - Oct 2005Y. Shionoya, D. Dupont, H. Hiranuma, S. Ando

Guide to HAISIL HL Part NumbersLx-xxxx-C185 HAISIL HL C18 5µmSee Page 23 for complete Part Number information

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HPLC Peptide Standards for RP onthe HAISIL HL C18 ColumnThe aromatic hydrocarbons that mostHPLC column manufacturers use toquality control columns render littleinformation about a column's selectivity

and performance characteristics forpeptides. The Canadian corporation, SPI,produces a well characterized yetchallenging peptide mixture that is veryuseful for quantifying HPLC columnperformance for peptides. The difficult toseparate first pair of peptides areresolved to near base line on HAISIL HLC18 columns. Many so-called peptideanalysis columns are unable to separatethis pair.

HA I S I L HL APPL ICAT IONS

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Speed and Selectivity for BallisticGradients on Complex LibrariesThe relative importance of particle sizeand selectivity are graphically illustratedbelow by the high speed analysis of a sixcomponent combinatorial library on aHAISIL HL C18 5µm column and twocompetitor’s C18 2µm columns. HAISILHL with its high carbon loading andsurface area of often works much betterthan expensive small particle sizecolumns that are prone to plugging upmore quickly.

Competitor “A”2µm C18

HAISIL HL5µm C18

Competitor “B”2µm C18

Water Soluble Vitamins

HAISIL HL C18 5µm 250x4.6mmA: 0.05mM KOAc, pH5; B: MeCNGradient: 2.5% - 30%B in 25min

Food AdditivesBeverage additive analysis is not only animportant industrial application, but italso serves as a practical and relativelysimple procedure for introductory HPLCcourses. Industry is increasingly driven byrapid analysis requirements and an HPLCcolumn can represent an overwhelmingexpense in a teaching environment. Thediet cola analysis in the chromatogram tothe left illustrates an extremely fastanalysis on an extremely inexpensiveHPLC column. A package containing five

of the 20x2.1mm cartridge column usedfor this analysis costs about half as muchas one moderately priced HPLC column.And, there's no need to pre-filter thecola sample through a 0.5µm filter assome column manufacturers recommendwhen using their expensive columnspacked with 2µm particles for thisanalysis! The readily available and non-toxic nature of the sample and the lowcost of Higgins Analytical cartridges hasmade the diet cola analysis an idealchoice for classroom purposes.

Basic Compounds in Combinatorial Mixtures

HAISIL HL C18P/N LS-1546-C185

Reverse Phase Chromatography ofBasic Compounds in CombinatorialMixturesIn the example at the top of the nextcolumn, A and B are single compoundscontaining two identical building blocks.A-B and B-A represent positionalisomers, each containing two buildingblocks.

This analysis was conducted on the crudeliquid-phase reaction mixture. Eachcomponent contains multiple secondary

and tertiary amine functionality yet thecombinatorial products can be rapidlychromatographed and exhibit no tailingon a 150x4.6mm HAISIL HL C18column using a simple linear 5% to 100%MeCN/water 0.1% TFA gradient.

Water Soluble Vitamin Analysis onHAISIL HL

1. H2N-RGAGGLGLGK-amide2. AC-RGGGGLGLGK-amide3. AC-RGAGGLGLGK-amide4. AC-RGVGGLGLGK-amide5. AC-RGVVGLGLGK-amide

“Hodges Test Peptide Mix”(from Synthetic Peptides, Inc.)

HAISIL HL C18 5µm 150x4.6mm P/N LS-1546-C185

Fast Diet Cola Analysis

HAISIL HL C18 5µm 2cm x 2.1mm Cartridge PN LK-0221-C185

15% MeCN/Water 200µL/min

Caffeine

Aspartame

Sodium Benzoate

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HEAVY

HEAVY Very Hydrophobic LoadingPhases C18

Particle Sizes 4.5µmPore Size 100Å

Pore Volume 1.1mL/gmSurface Area 450m2/gm

%Carbon (w/w) C18 = 22%Phase type Monofunctional & fully endcappedSilica Class Type B

ApplicationsKeeping in mind that relative retention(α) is a much more powerful term thanplates (N) in the expression for resolu-tion in chromatography, choosing a col-umn with different selectivity is almostalways more helpful than just going to asmaller particle size when trying toimprove a difficult separation. Our newHEAVY columns provide the highestretention of any column we manufacture.

Relative hydrophobicity of Higgins Analytical’s bonded phase sorbents.Contitions: 70% MeCN/Water

Guide to HEAVY Part NumbersVx-xxxx-C185 HEAVY C18 5µmSee Page 23 for complete Part Number information

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Using a PICCOLO as a MicroboreGuard ColumnPICCOLO columns are normally used astrap columns but since they aremanufactured with the same techniques

we use to pack microbore HPLCcolumns, they are very efficient. Theirperformance makes them ideal for useas short microbore HPLC column or asa guard for placed in front of anotheranalytical microbore column. Thechromatograms in the figure belowillustrate typical performance when usedin these unconventional modes.

Why do HPLC columns plug up? A large percentage of HPLC columns diebecause the back pressure builds up to

levels that exceed the instrument’spractical limits. Most chromatographers(and many column manufacturers)believe this is because the inlet frit plugsup with particles. Actually, it is usually thepacked-bed that becomes plugged bysmaller than 1µm particles that passthrough in-line filters and the 2µm inletfrits. The SEM above illustrates therelative sizes of particles and pores.Higgins Analytical offers a wide variety ofguard cartridges that address thisproblem.

13

INTERE ST ING COLUMN FACTS

The Role of Column Length inGradient Separations Changes in column length have a directand linear effect on theoretical platesand analysis time in isocratic separa-tions. Column length plays a lessimportant role, however, in gradienttechniques. The three chromatogramsbelow illustrate how well packed shortHPLC columns yield very similarpeptide separations under identicalgradient conditions.

For this study, the 2.1mm I.D. HAISIL300 C18 5µm columns ranged from250 to 100mm in length. While solventand analysis time savings may beminimal, column cost savings can besignificant when using as short a columnas possible. Short columns also exhibitlower pressure at high flow rates.

250x2.1mmHAISIL 300 C18 5µmHS-2521-W185

150x2.1mmHAISIL 300 C18 5µmHS-1521-W185

100x2.1mmHAISIL 300 C18 5µmHS-1021-W185

1. Gly-Tyr2. Val-Tyr-Val3. Met Enkephalin4. Leu Enkephalin5. Angiotensin II

5µm Silica Gel Particle

0.75µm Extra-Particle Flow Path

Piccolo Column performance as anAnalytical Column and as a Guard Column

Blue Trace: 10x1mm Targa C18 5µm Piccolo GuardColumn alone (P/N TP-0101-C185)

Red Trace: 50x1mm TARGA C18 5µm AnalyticalColumn (P/N TS-0501-C185)

Black Trace: 10x1mm Guard coupled to 50x1mmAnalytical Column

Peak #1: Dimethyl Phthalate Peak #2: Fluorene60% MeCN/water, 50µL/min

System Variance ~6µL2 at 50µL/min

SPRITE is Designed to Operate inEither Flow DirectionA SPRITE is made of PEEK and titaniumand can be connected directly to asample injection valve or directly to amass spectrometer’s source inlet.

Page 16: HAI Price List 2005 - HPLC Column Manufacturer

PROTO™ 200 More Selectivity and Resolution for Peptides

Phases C4 and C18Particle Sizes 4.5 and 10µm

Pore Size 200ÅPore Volume 1.1mL/gmSurface Area 200m2/gm

%Carbon (w/w) C4. = 4.5%, C18= 14%Phase type Monofunctional & fully endcappedSilica Class Type B

14

PROTO200

Applications300Å wide pore HPLC columns aretraditionally used for biomolecule analysissince the molecular radii of largepeptides and proteins are bettermatched than with smaller pore sizecolumns (80 - 120Å) typically used forsmall molecule analysis. 300Å sorbents,however, have reduced surface area, thusless capacity and selectivity than wouldbe had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200presents a powerful alternative for theanalysis of peptides of ~19kD and less.

200

PROTO 200150x4.6mm

P/N/ RS-1546-D185

238EV150x4.6mm

218TP250x4.6mm

Phosphopeptide Purity AnalysisPeptide HPLC column performance,using the same gradient conditions, wasevaluated on the PROTO™ 200 C18, anew peptide analysis column obtainedfrom The Nest Group, Inc.. Against theindustry standard, Grace/Vydac® 218TPC18, and their newer peptide chemistry,Everest® C18, the data show that thePROTO™ 200 C18 column has superiorresolution to the 218TP chemistry andbetter resolution, especially for the morehydrophobic peptides, than the Everest®C18. In addition, the PROTO 200columns can be run without TFA, whichallows better detection when utilizingLC-MS.

Previously, the Everest chemistry hadbeen shown by Grace/Vydac to besuperior to all other wide pore columns,especially for the more hydrophobicpeptides. However, the ready availabilityof the PROTO™ 200 peptide columns,in either C4 or C18 chemistries, and incapillary through preparative sizes, nowallows researchers their first opportunityto save both time and money on a widervariety of applications, compared to theGrace/Vydac columns, and by extensionto all other peptide columns.

Comparison of Phosphopeptide Purity Analysis

Guide to PROTO 200 Part NumbersRx-xxxx-D045 PROTO 200 C4 5µmRx-xxxx-D185 PROTO 200 C18 5µmSee Page 23 for complete Part Number information

200

Page 17: HAI Price List 2005 - HPLC Column Manufacturer

PROTO200

15

Vydac C18 238MS5115150 x 1.0mm

PROTO 200 C18150 x 1.0mm

Proto™ 200 C18 provides greater selectivity than the popular 300Å choice

Tryptic Digest of Carbamidomethylated BSA (16h, 20 pMols on column).

Column Size: 150 x 1.0mm

Column Brand:Upper Chromatogram: Vydac 300Å 238MS C18 5µm P/N 238MS5115Lower Chromatogram: Higgins 200Å Proto 200 C18 5µm P/N RS-1501-D185

Conditions:A = 0.1% formic acid containing 0.01% TFAB = 0.085% formic acid in CH3CN containing 0.01% TFAInitial 5 min isocratic hold at 5% B, then 60 min hyperbolic gradient to 65%BFlow 0.15 ml/min, Green & Black curves: Blank gradient profile

Higgins Analytical thanks Amos Heckendorf of The Nest Group (www.nestgrp.com) for these Proto 200 data.

200

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16

PROTO 300

PROTO™300 Robust BioseparationsPhases C4 and C18

Particle Sizes 4.5 and 10µmPore Size 300Å

Pore Volume 0.9mL/gmSurface Area 100m2/gm

%Carbon (w/w) C4 = 3%, C18= 8%Phase type Monofunctional & fully endcappedSilica Class Type B

ApplicationsLow pH operating conditions and highpH column washing steps are typicalbioprocessing operating conditions thatcan severely limit the lifetime andperformance of widepore columnstypically used for protein and peptideanalysis. Higgins Analytical’s newPROTO300 has high capacity forenhanced resolution and is robustenough to withstand prolonged use atextreme pH (1.5 - 10).

Proto™300 C18

Manufacturer K300 C18

Manufacturer P300 C18

Stability Study Under ExtremelyAlkaline Conditions Experimental:150 x 4.6mm columns were studied. Allexperiments were conducted onduplicate columns. Solutes used forperformance measurement weredimethylphthalate and fluorene.

Step One:Column stability and measurementprecision was established for eachcolumn with multiple injections over18hrs under neutral conditions (70%MeCN/water, 1mL/min). All columnswere very stable. The chromatograms ofa column from each manufacturer at theend of the 18 hour period are shown onthe left hand side.

Step Two:Each column was flushed with 70%MeCN/0.5N NaOH solution at 1mL/minfor one hour (23ºC). The columns werethen purged and equilibrated under thestandard neutral conditions thenretested. The center chromatograms arethe results of the first test after neutralequilibration

Step Three:After the second step, the neutral testconditions were maintained for one hourand a repeat injection was made. Theresults are illustrated in thechromatograms on the right hand side ofthe figure.

While Higgins Analytical does notrecommend flushing any silica-basedHPLC column under such aggressiveconditions, these data show the superiorakaline stability of Proto™ 300 C18.

Guide to PROTO 300 Part NumbersRx-xxxx-W045 PROTO 300 C4 5µmRx-xxxx-W185 PROTO 300 C18 5µmSee Page 23 for complete Part Number information

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PROTO 300

17

C8 Vydac®, 208TP5215, 5µm2.1 x 150mm

C4 PROTO™300, 5µm2.1 x 250mm

C8 Zorbax® Rapid Resolution 3.5µm2.1 x 150mm

Column: 250 x 2.1mm Higgins P/N RS-2521-W045 (5µm, 300Å)Vydac P/N 214TP52 (5µm, 300Å)

Mobile Phase: (A) 0.1%TFA in water, (B) 0.08%TFA in ACN, Flow Rate: 0.3minStart Conditions: 25%B, Linear Gradient Run Time 34min

Superior Resolution with PROTO™300 C4 for Protein Tryptic Digests

PROTO™ 300 C4 for Protein Analysis

Higgins Proto 300 C4250 x 2.1mm

Vydac 214TP52 C4250 x 2.1mm

Higgins Analytical thanks Amos Heckendorf of The Nest Group (www.nestgrp.com) for these data.

Page 20: HAI Price List 2005 - HPLC Column Manufacturer

18

Phalanx is Higgins Analytical’s latest addi-tion to a family of unique high perform-ance HPLC sorbents. The novel end cap-ping technology of this new sorbent is acontinuation of the pioneering approachtaken in 1996 when we introducedTarga®, the first HPLC sorbentspecifically designed for efficientLC-MS applications without TFA.

Based on an ultrapure, Type-B silica back-bone, Phalanx has a relatively high sur-face area of 430 m2/gm, but its very tightpore size distribution eliminates theundesirable side effects of microporesthat often plague other high surfacematerials.

Optimum for Basic and Acidic com-pounds: Many modern HPLC sorbentsemploy embedded polar functionality.While these secondary structures canprovide unique selectivity and eliminateundesirable solute/sorbent interaction,they often compromise the useful rangeof solutes or buffers that can be used.The figure on the left and those on page2 illustrate that Phalanx columns pro-duce symmetrical peaks during the analy-sis of basic, neutral, and acidic com-pounds.

Wide pH operating range: The uniqueendcapping reduces acid/base hydrolysisof the bonded phase and greatly reducessilica solvation at high pH.

PHALANX

PHALANX Ultra High Density End CappingPhases C18

Particle Sizes 3, 4.5, 10µmPore Size 100Å

Pore Volume 1.1mL/gmSurface Area 430m2/gm

%Carbon (w/w) C18 = 18%Phase type Monofunctional & fully endcappedSilica Class Type B

ApplicationsPhalanx is uniquely suitable for theefficient analysis of both acidic and basicanalytes. In addition, its high capacity, goodstability at high pH, and fast equilibrationat extreme ranges of hydrophobicitymake Phalanx a powerful option forchallenging separation problems.

Phalanx HPLC columns and cartridges facilitate the development ofrobust assays for challenging analytes

Unique Features:• Ideal for Basic and Acidic Analytes• Wide pH Range Stability• High Capacity• No secondary Si-OH interaction• No metal contamination• Extreme hydrophobicity range

Standard Sizes:• Special products for LC-MS• 0.075 - 20mm I.D. range• Wide range of HPLC Cartridge and Column types• PEEK/titanium as well as stainless steel formats

High SelectivityWide range of solutes resolve quickly on a short HPLC columnPhalanx C18, 100x4.6mm, P/N: PS-1046-C185(1) Uracil, (2) Caffeine, (3) Phenol, (4) n-Butylbenzene, (5) o-Terphenyl, (6) n-Amylbenzene, (7) Triphenylene80% MeOH/water, 1mL/min

Guide to Phalanx Part NumberPx-xxxx-C185 Phalanx C18 5µmSee Page 23 for complete Part Number information

1

2

3

45

6

7

NEW

3 & 10

µm

Page 21: HAI Price List 2005 - HPLC Column Manufacturer

19

Good Peak Symmetry for BasicCompounds

The very low solute/sorbent interactionof Phalanx C18 is clearly illustrated bythe analysis of basic compounds on ashort 5cm cartridge column. Phalanx C18, 50x2.1mm, P/N: PK-0521-C185(1) Propanediol, (2) Diphenyldramine,(3) Acenapthene, (4) Amitiptyline.65% MeOH/water 20mM KH2PO4 (pH 7.0), 200µL/min

Tricyclic Antidepressants

The very low solute/sorbent interactionof Phalanx C18 is clearly illustrated bythe analysis of basic compounds on a100x4.6mm HPLC column. Phalanx C18, 100x4.6mm, P/N: PS-1046-C185(1) Desipramine, (2) Nortriptyline, (3) Acenaphthene,(4) Imipramine, (5) Amitriptyline, (6) Trimipramine.70% MeOH/water 20mM KH2PO4 (pH 7.0), 1.0mL/min

Unbuffered Pyridine Test for Free Silanol Activity

A very sensitive test for residual silanolsproduces a symmetrical pyridine peak ina low organic mobile phase withoutbuffers. Phalanx C18, 50x2.1mm, P/N: PK-0521-C185(1) Uracil, (2) Phenol, (3) Pyridine40% MeCN/water, 200µL/min

NIST Test Mix for Metal Activity andResidual Silanols

Quininzarin, a strong metal chelator, andamitriptyline, a very sensitive solute forresidual silanols, both elute with goodrecovery and symmetrical peaks on bothTarga and Phalanx C18 columns. Targa® C18, 150x4.6mm, P/N: TS-1546-C185Phalanx™ C18, 150x4.6mm, P/N: PS-1546-C185(1) Uracil, (2) Toluene, (3) Ethylbenzene,(4) Quinizarin,(5) Amitiptyline.80% MeOH/buffer, 1.5mL/min

Nucleosides

Like its cousin Targa, Phalanx’s uniqueend-capping chemistry works equallywell at extreme hydrophilic andhydrophobic ranges. Phalanx C18, 50x2.1mm, P/N: PK-0521-C185(1) Cytosine, (2) Uracil, (3) Cytidine, (4) Uridine, (5) Thymine.100% Water, 1.0mL/min

TARGA® C18

PHALANX™ C18

1

2

3

4

1 2 3

4

5

1 2 3

4

5

1

2

3

45

1

2

3

45

6

1

2

3

PHALANX APPL ICAT IONS

See page 28 for an interestingcomparison of PHALANX toKROMASIL C18 for an analysis of apharmaceutical drug.

Page 22: HAI Price List 2005 - HPLC Column Manufacturer

20

While TARGA columns give exceptionalperformance without TFA buffer, it doesnot mean that you are precluded fromusing it. In fact, the changes in selectivity

that comes with changing buffer acidscan be a powerful tool for separationoptimization. Notice how the peptideelution orders change between theacetic acid (above) and TFA (below)buffered chromatogram. This is due tothe diferences in pH of the acetic acidand TFA mobile phase.

TARGA

TARGA A Uniquely Shielded Surface Phases C8 and C18

Particle Sizes 3, 4.5 and 10µmPore Size 120Å

Pore Volume 0.8mL/gmSurface Area 330m2/gm

Carbon%(w/w) C18 = 18%Phase type Monofuctional with a unique polar and

bulky end-cappingSilica Class Type B

ApplicationsTARGA columns and cartridges areideal for applications requiring anabsence of secondary solute-silanol inter-action. Basic and acidic drugs and pep-tides can be chromatographed efficientlywith little or no buffer. Targa columns areparticularly well suited for LC/MS appli-cations where acetic acid or formic acidcan be substituted for TFA resulting insignificant increase in signal. While TFA isnot required, it does not preclude its useon TARGA columns. TARGA exhibitsextremely fast equilibration kinetics fromhigh to low organic phase compositions.It works well in 100% aqueous condi-tions for hydrophilic applications also.Peptide Mapping without TFA

The following chromatograms illustratethe differences in selectivity for peptidemaps of cytochrome-C when using0.05% TFA or 0.05% acetic acid buffer in

1%/min acetonitrile gradient on a short50x1.0mm TARGA C18 column. Bothdilute buffer examples are characterizedby sharp peaks and no tailing.

LC-MS without TFAResearchers have shown that HigginsAnalytical’s TARGA C8 and C18 phaseshave an extremely well protected silanolsurface. An acetonitrile/water 1%/mingradients with as little as 0.05% aceticacid gives a very satisfactory peptidemap on a short 5cm x 1mm TARGAC18 column. The ability to buffer withacetic or formic acid rather than TFAgreatly enhances signals for LC/MS tech-niques.

Basic Peptides

with 0.1% Acetic Acid pH 3.1TARGA C18 5µm 50x1.0mm

Basic Peptides

with 0.1% TFA pH 2.2TARGA C18 5µm 50x1.0mm

Tryptic Map of Human Ocular Lens Proteins beta A1 crystallin and gamma S crystallin

TARGA C18 5µm 150x0.5mmP/N TS-15M5-C185

Solvent A: 0.1% Acetic AcidSolvent B: 0.1% Acetic Acid, 65% MeCN0 - 50% B in 60min, 50 - 100%B in 15min

Sample is a tryptic digest of a 2D electrophoresis separat-ed spot digested from PDVF membrane. The two ocularproteins are ~25kD. The LC/MS zoom scan below con-firms the peak at 39.15min is singly charged, and softwareinterpretation of the MS/MS scan at the bottom confirmsthe 1156.6 ion as residues 84-94 in human gamma S crys-tallin.

LC/MS Scan

LC/MS/MS Scan

Guide to TARGA Part NumbersTx-xxxx-C183 Targa C18 3µmTx-xxxx-C185 Targa C18 5µmSee Page 23 for complete Part Number information

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Unrivaled End Capping TechnologyEfforts to eliminate solute-silanol Targa HPLC columns are true to theLatin root of their name. TARGA,meaning “shield,” is precisely the effectthe unique bonded phase has on thesilica’s silanol surface.

Many HPLC column manufacturersillustrate the performance of theircolumns packed with inert, base deacti-vated, “Type B silica” bonded phases bythe chromatographic behavior of polarcompounds such as pyridine.Performance claims can be misleading,however, when mobile phases arebuffered to low pH or when theycontain high percentages of organicsolvent.

Favorable Kinetics at Aqueous orOrganic Mobile Phase ExtremesThe bulky moiety used for end cappingTARGA C8 and C18 sorbents presentother valuable selectivity characteristics inaddition to the extreme inertness tobasic and acidic compounds. Long chainalkane bonded phases collapse underlow organic phase conditions on mostreverse phase HPLC columns causingpoor reproducibility and slow reequilibra-tion. Fast gradients can be run onTARGA columns from initial 100%aqueous conditions with fast cycle timesand very good run-to-run reproducibility.TARGA columns can also be run isocrat-ically in 100% aqueous conditions forcarbohydrate analysis, or with only a fewpercent organic modifier to achieveselectivity on solutes that are retainedunder typical reverse phase conditions.

TARGA APPL ICAT IONS

Trace Catecholamine AnalysisIn vivo microdialysis present a challengeto both instrumentation and columndesign, especially when depended uponas a tool for routine analysis. Thechromatogram below illustrates rathypothalamus microdialysis analysis for5HT. Researchers report that the100x2.1mm Higgins Analytical PEEK andtitanium TARGA C18 column operatesroutinely at 0.2 - 100 nM detectionlevels.

5HT Analysis of Rat Hypothalamus Dialysate

TARGA C18 5µm 100x2.1mmP/N TS-1021-C185

NaH2PO4 0.15M, OSA 0.74mM, EDTA 0.1mMpH 2.87 (orthophosphoric acid) 2% IPA

Instrumentation: Flux Rheos Isocratic Pump, In-line SolventDegasser, Spark Holland Triathlon Autosampler, AntecINTRO EC Detector

Detection levels of 0.2 - 100 nM are routinely achieved.

TARGA Compared to a Competitor’s Hydophilic Column

150x4.6mm, 20% MeCN/water (unbuffered) 1mL/min

TARGA’s Superior HydrophilicReverse Phase PropertiesSeveral manufacturers offer reversephase column products with imbeddedpolar functionality intended to enhancethe chromatography of hydrophiliccompounds like those illustrated in theprevious antibacterial example.Unfortunately, most of these approachesintroduce undesirable secondary effectsthat preclude their use with acidic orbasic solutes unlike TARGA with itsunique bonded phase.

Fat-Soluble Vitamin Assay

TARGA C18 5µm 100x2.1mmPart Number TS-1021-C185

3% water in MeOH mobile phase200µl/min, diode array detection

Analysis of Very Polar Antibacterials with a Highly Aqueous Mobile Phase

1. Vancomycin, 2. Cefotaxime, 3. Cefazolin

TARGA C18 100x2.1mm TS-1021-C185Mobile Phase: 92% Water with 0.l% TFA pH2.5,

8% MeOH, DAD at 230nm

Unbuffered 20% MeCN/water

Anti-Hypertensives

TARGA C18 5µm 150x4.6mmPart Number TS-1546-C185

25% MeCN/20mM NaH2PO4 pH3.2 1mL/min 260nm

1. Triamterene 2. Hydrochlorothiazide

Analgesics and Anti-Inflammatorys

TARGA C18 5µm 150x4.6mmPart Number TS-1546-C185

45% MeCN/20mM NaH2PO4 pH3.2 1mL/min 260nm

1. Acetaminophen 2. Indoprofen3. Naproxan 4. Indomethacin5. Ibuprofen

A Popular Hydrophilic RP Column

TARGA C18 Column

Pyridine

Pyridine Phenol

Phenol

Page 24: HAI Price List 2005 - HPLC Column Manufacturer

We Manufacture Products with Internal Diameters Ranging Almost Three Orders of MagnitudeThis catalog contains over one thousand products manufactured with over fifteen different phases in an unequaled variety of for-mats. This generous offering makes it easy to choose the proper column for your application.

The columns and cartridges included in this version of the Higgins Analytical cat-alog have internal diameters ranging from 75µm to 20mm. Not listed are our30mm and 50mm i.d. prep-columns. The bulk of our business in these large for-mats is based on Proto 200 and Proto 300 C4 and C18 products on particlesizes ranging from 10µm to 50µm geared for biomolecule purification - especiallysynthetic peptides.

As outlined in the paragraph below, another service we provide is custom manu-facturing for columns of all sizes, 30mm and 50mm prep-columns included, withsorbents produced by manufacturers other than ourselves. So, if you are interest-ed in one of our many sorbents, or another material that you may prefer, pleasegive us a call to discuss the details.

UNEQUALED FLEXIBILITY

22

S P EC IALTY COLUMNS AND S ERV ICE S

PTC Amino Acid AnalysisHiggins Analytical now manufacturers the PTC Amino Acid analysis column used on the Applied Biosystems Model 420 Analyzer.We have approval from AB to manufacture these columns and we can provide a certificate indicating that our columns have theidentical form and function as the product previously supplied by AB.

Part Number: 0711-0204 Price: $600 Always available from stock.

A recent publication cites a Higgins Analytical PTC column: Mass-spectrometric study of changes in allergen proteins by application ofcontrolled chemical reaction, where attempts to identify stable peptide markers from hazelnut and soybean were made.

C. Platteau, T. Cucu, R. Dobson, S. Fourdrilis, C. Bourgeon, D. Ebo, M. De Loose, I.Taverniers, E. Daeseleire, B. De Meulenaer, B. Devreese, E. DePauw, G. Maghuin-Rogister, M.-L. Scippo, W. Stevens, C. Bridts. “Development of an integrated strategy for controlling the allergen issue in theBelgian food and catering industry ALLERRISK”. Final Report. Brussels : Belgian Science Policy 2011 – 109 p. (Research Programme Sciencefor a Sustainable Development), Page 54

http://www.belspo.be/belspo/SSD/science/Reports/ALLERRISK_FinRep%20ML.pdf

Custom Manufacturing ServiceBesides being known worldwide for our huge variety of high quality and uncompromisingly affordable Higgins Analytical products,custom labeled manufacturing represents a sizeable part of our business. In fact, a lot of people might be very surprised if theylearned that their favorite competitive column was actually packed by Higgins Analytical. In addition to being based on our ownunique sorbents, many of the products we manufacture are produced with our customers’ own proprietary media. So, if your com-pany feels it might want to add a selection of high quality HPLC columns to its product offering, or if you need large numbers of aparticular column or device for your internal analytical needs, please give us a call. Higgins Analytical is very expert at providing thistype of service.

If you can put it in a bottle, Higgins Analytical will pack it into a column. If we’re unsuccessful, it won’t cost a thing!

Page 25: HAI Price List 2005 - HPLC Column Manufacturer

23

GU IDE TO H IGG INS ANALYT ICAL’ S PART NUMBER ING SYSTEM

HPLC Columns and CartridgesHiggins Analytical HPLC column productpart numbers are based on a 2-4-4character pattern. For example:

TS-1521-C185

is the part number for a conventional15cm x 2.1mm stainless steel columnpacked with Targa C18 5µm sorbent.

The two character prefix indicates sorbent and hardware type.

Prefix Character 1 (Sorbent)X-XXXX-XXXX

C = CLIPEUSH = HAISIL 100 or HAISIL 300L = HAISIL HLP = PHALANXR = PROTO 200 or 300T = TARGAV = HEAVY

Prefix Character 2 (Hardware)X-XXXX-XXXX

FS = Capellini 6-32 columnPS = Capellini 10-32 columnS = Stainless steel HPLC ColumnP = PEEK HPLC columnF = Stainless steel cartridge columnK = PEEK cartridge columnR = Sprite Direct-Connect column

Note: Microbore columns have the sec-ond character, S, like stainless steelcolumns, but they are glass lined.

The four center characters indicate thecolumn or cartridge length (cm) and internal diameter (mm).

Center Characters 1 and 2 (Length)XX-XX-XXX

03 = 3cm04 = 4cm05 = 5cm07 = 7.5cm10 = 10cm12 = 12.5cm15 = 15cm20 = 20cm22 = 22cm25 = 25cm30 = 30cm50 = 50cm

The four center characters indicate the column or cartridge length (cm) and internal diameter (mm).

Center Characters 3 and 4 (I.D.)XX-XX-XXXX

07 = 0.075mmM1 = 0.1mm15 = 0.15mmM3 = 0.3mmM5 = 0.5mmM8 = 0.8mm01 = 1.0mm21 = 2.1mm30 = 3.0mm32 = 3.2mm39 = 3.9mm40 = 4.0mm46 = 4.6mm10 = 10mm20 = 20mm

The four character suffix indicates sorbentchemistry and particle size.

Suffix Characters 1 to 3 (Phase)XX-XXXX-X

C04 = Butyl or C4C08 = Octyl or C8C18 = ODS or C18CNP = Cyano or NitrileD04 = 200Å C4D18 = 200Å C18M08 = HAISIL 100 C8M18 = HAISIL 100 C18NH2 = AminoPHN = PhenylSIL = Silica Gel W04 = 300Å C4W08 = 300Å C8W18 = 300Å C18

Suffix Character 4 (Particle Size)XX-XXXX-XXX

1 = 10µm2 = 10 - 15µm3 = 3 or 3.5µm5 = 4 - 5µm7 = 15 - 20µm

Notes: (1) Standard configurations are highlighted.(2) Polymeric phases have a 3-4-6 pattern.

The first two prefix charactes are PL.The last character denotes particle size.

PLx-xxxx-xxxxxx

Cartridge HoldersGuardsTwo types of holders for 2cm long PEEKand s.s. cartridges are offered.

HK-GUARD-FF Female/FemaleHK-GUARD-FM Direct Connect

Analytical Cartridge HoldersThree internal diameters (2.1, 3.2, and4.6mm) and three lengths are offered.

HK-HOLD-40 for 4cm long cartridgeHK-HOLD-50 for 5cm long cartridgeHK-HOLD-100 for 10cm long cartridge

HAISpeed 10 Cartridge HoldersThese 10mm i.d. cartridges are offered inthree lengths.

10-HOLD-30 for 3cm long cartridge10-HOLD-50 for 5cm long cartridge10-HOLD-100 for 10cm long cartridge

HAISpeed 20 Cartridge Holder20mm i.d. prep guard cartridges require a25mm long cartridge holder.

20-HOLD-25 for 20 x 25mm cartridge

Page 26: HAI Price List 2005 - HPLC Column Manufacturer

24

HPLC ColumnsWide Range of Stainless Steel HPLC COLUMNS

Widest Range of Hardware Typesand Sizes Available AnywhereHiggins Analytical manufacturers thewidest range of column formats in thewidest range of hardware types availableanywhere. Conventional HPLC columnsare offered in stainless steel hardware.Frit materials include stainless steel,Carpenter C, titanium. Standard lengthsrange from 3.3cm to 25 cm. Customcolumns are routinely manufactured inodd length ranging from 3.3 to 50cm.The internal diameters of our entireHPLC column and cartridge offeringrange from 75µm to 20mm, over twoorders of magnitude.

Guide to Some Standard HPLC Column Part NumbersxS-xxxx-xxxx Stainless Steel (ss) columnxP-xxxx-xxxx PEEK columnxS-03xx-xxxx 3.5cm ss column Shortest standard sizexS-25xx-xxxx 25cm ss column Longest standard sizexP-05xx-xxxx 5cm PEEK column Shortest standard sizexP-25xx-xxxx 25cm PEEK column Longest standard sizexS-xx21-xxxx 2.1mm ss I.D. Smallest standard I.D.xP-xx21-xxxx 2.1mm PEEK I.D. Smallest standard I.D.xS-xx46-xxxx 4.6mm ss I.D. Popular standard I.D.xP-xx46-xxxx 4.6mm PEEK I.D. Popular standard I.D.See Page 23 for complete Part Number information

CLIPEUS Analytical Columns - Stainless SteelCLIPEUS C8 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-C085 $270 CS-0546-C085 $280 CS-1046-C085 $290 CS-1546-C085 $325 CS-2546-C085 $35030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmCS-0340-C085 $270 CS-0540-C085 $280 CS-1040-C085 $290 CS-1540-C085 $325 CS-2540-C085 $35030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmCS-0330-C085 $270 CS-0530-C085 $280 CS-1030-C085 $290 CS-1530-C085 $325 CS-2530-C085 $35030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmCS-0321-C085 $270 CS-0521-C085 $280 CS-1021-C085 $290 CS-1521-C085 $325 CS-2521-C085 $350

CLIPEUS C18 10µm 4.6mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-C181 $245 CS-0546-C181 $260 CS-1046-C181 $270 CS-1546-C181 $295 CS-2546-C181 $320

CLIPEUS C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-C185 $270 CS-0546-C185 $280 CS-1046-C185 $290 CS-1546-C185 $325 CS-2546-C185 $35030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmCS-0340-C185 $270 CS-0540-C185 $280 CS-1040-C185 $290 CS-1540-C185 $325 CS-2540-C185 $35030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmCS-0330-C185 $270 CS-0530-C185 $280 CS-1030-C185 $290 CS-1530-C185 $325 CS-2530-C185 $35030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmCS-0321-C185 $270 CS-0521-C185 $280 CS-1021-C185 $290 CS-1521-C185 $325 CS-2521-C185 $350

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CLIPEUS Analytical Columns - Stainless SteelCLIPEUS Cyano 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-CNP5 $280 CS-0546-CNP5 $290 CS-1046-CNP5 $300 CS-1546-CNP5 $345 CS-2546-CNP5 $36530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmCS-0340-CNP5 $280 CS-0540-CNP5 $290 CS-1040-CNP5 $300 CS-1540-CNP5 $345 CS-2540-CNP5 $36530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmCS-0330-CNP5 $280 CS-0530-CNP5 $290 CS-1030-CNP5 $300 CS-1530-CNP5 $345 CS-2530-CNP5 $36530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmCS-0321-CNP5 $280 CS-0521-CNP5 $290 CS-1021-CNP5 $300 CS-1521-CNP5 $345 CS-2521-CNP5 $365

CLIPEUS Phenyl 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-PHN5 $280 CS-0546-PHN5 $290 CS-1046-PHN5 $300 CS-1546-PHN5 $345 CS-2546-PHN5 $36530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmCS-0340-PHN5 $280 CS-0540-PHN5 $290 CS-1040-PHN5 $300 CS-1540-PHN5 $345 CS-2540-PHN5 $36530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmCS-0330-PHN5 $280 CS-0530-PHN5 $290 CS-1030-PHN5 $300 CS-1530-PHN5 $345 CS-2530-PHN5 $36530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmCS-0321-PHN5 $280 CS-0521-PHN5 $290 CS-1021-PHN5 $300 CS-1521-PHN5 $345 CS-2521-PHN5 $365

CLIPEUS Silica 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmCS-0346-SIL5 $245 CS-0546-SIL5 $250 CS-1046-SIL5 $260 CS-1546-SIL5 $295 CS-2546-SIL5 $31530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmCS-0340-SIL5 $245 CS-0540-SIL5 $250 CS-1040-SIL5 $260 CS-1540-SIL5 $295 CS-2540-SIL5 $31530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmCS-0330-SIL5 $245 CS-0530-SIL5 $250 CS-1030-SIL5 $260 CS-1530-SIL5 $295 CS-2530-SIL5 $31530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmCS-0321-SIL5 $245 CS-0521-SIL5 $250 CS-1021-SIL5 $260 CS-1521-SIL5 $295 CS-2521-SIL5 $315

HAISIL100 Analytical Columns - Stainless SteelHAISIL 100 C8 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmHS-0346-M085 $270 HS-0546-M085 $280 HS-1046-M085 $290 HS-1546-M085 $325 HS-2546-M085 $35030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmHS-0340-M085 $270 HS-0540-M085 $280 HS-1040-M085 $290 HS-1540-M085 $325 HS-2540-M085 $35030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmHS-0330-M085 $270 HS-0530-M085 $280 HS-1030-M085 $290 HS-1530-M085 $325 HS-2530-M085 $35030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmHS-0321-M085 $270 HS-0521-M085 $280 HS-1021-M085 $290 HS-1521-M085 $325 HS-2521-M085 $350

HAISIL 100 C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmHS-0346-M185 $270 HS-0546-M185 $280 HS-1046-M185 $290 HS-1546-M185 $325 HS-2546-M185 $35030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmHS-0340-M185 $270 HS-0540-M185 $280 HS-1040-M185 $290 HS-1540-M185 $325 HS-2540-M185 $35030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmHS-0330-M185 $270 HS-0530-M185 $280 HS-1030-M185 $290 HS-1530-M185 $325 HS-2530-M185 $35030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmHS-0321-M185 $270 HS-0521-M185 $280 HS-1021-M185 $290 HS-1521-M185 $325 HS-2521-M185 $350

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HAISIL100 Analytical Columns - Stainless Steel (cont.)HAISIL 100 Silica 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmHS-0346-SIL5 $245 HS-0546-SIL5 $250 HS-1046-SIL5 $260 HS-1546-SIL5 $295 HS-2546-SIL5 $31530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmHS-0340-SIL5 $245 HS-0540-SIL5 $250 HS-1040-SIL5 $260 HS-1540-SIL5 $295 HS-2540-SIL5 $31530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmHS-0330-SIL5 $245 HS-0530-SIL5 $250 HS-1030-SIL5 $260 HS-1530-SIL5 $295 HS-2530-SIL5 $31530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmHS-0321-SIL5 $245 HS-0521-SIL5 $250 HS-1021-SIL5 $260 HS-1521-SIL5 $295 HS-2521-SIL5 $315

HAISIL300 Analytical Columns - Stainless SteelHAISIL 300 C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmHS-0346-W185 $310 HS-0546-W185 $320 HS-1046-W185 $350 HS-1546-W185 $375 HS-2546-W185 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmHS-0340-W185 $310 HS-0540-W185 $320 HS-1040-W185 $350 HS-1540-W185 $375 HS-2540-W185 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmHS-0330-W185 $310 HS-0530-W185 $320 HS-1030-W185 $350 HS-1530-W185 $375 HS-2530-W185 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmHS-0321-W185 $310 HS-0521-W185 $320 HS-1021-W185 $350 HS-1521-W185 $375 HS-2521-W185 $405

HAISIL HL Analytical Columns - Stainless SteelHAISIL HL C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmLS-0346-C185 $380 LS-0546-C185 $390 LS-1046-C185 $400 LS-1546-C185 $425 LS-2546-C185 $44030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmLS-0340-C185 $380 LS-0540-C185 $390 LS-1040-C185 $400 LS-1540-C185 $425 LS-2540-C185 $44030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmLS-0330-C185 $380 LS-0530-C185 $390 LS-1030-C185 $400 LS-1530-C185 $425 LS-2530-C185 $44030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmLS-0321-C185 $380 LS-0521-C185 $390 LS-1021-C185 $400 LS-1521-C185 $425 LS-2521-C185 $440

HEAVY Analytical Columns - Stainless SteelHEAVY C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmVS-0346-C185 $310 VS-0546-C185 $320 VS-1046-C185 $350 VS-1546-C185 $375 VS-2546-C185 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmVS-0340-C185 $310 VS-0540-C185 $320 VS-1040-C185 $350 VS-1540-C185 $375 VS-2540-C185 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmVS-0330-C185 $310 VS-0530-C185 $320 VS-1030-C185 $350 VS-1530-C185 $375 VS-2530-C185 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmVS-0321-C185 $310 VS-0521-C185 $320 VS-1021-C185 $350 VS-1521-C185 $375 VS-2521-C185 $405

HPLC Columns (continued)Wide Range of Stainless Steel HPLC COLUMNS

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PROTO 200 Analytical Columns - Stainless SteelPROTO 200 C4 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmRS-0346-D045 $310 RS-0546-D045 $320 RS-1046-D045 $350 RS-1546-D045 $375 RS-2546-D045 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmRS-0340-D045 $310 RS-0540-D045 $320 RS-1040-D045 $350 RS-1540-D045 $375 RS-2540-D045 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmRS-0330-D045 $310 RS-0530-D045 $320 RS-1030-D045 $350 RS-1530-D045 $375 RS-2530-D045 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmRS-0321-D045 $310 RS-0521-D045 $320 RS-1021-D045 $350 RS-1521-D045 $375 RS-2521-D045 $405

PROTO 200 C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmRS-0346-D185 $310 RS-0546-D185 $320 RS-1046-D185 $350 RS-1546-D185 $375 RS-2546-D185 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmRS-0340-D185 $310 RS-0540-D185 $320 RS-1040-D185 $350 RS-1540-D185 $375 RS-2540-D185 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmRS-0330-D185 $310 RS-0530-D185 $320 RS-1030-D185 $350 RS-1530-D185 $375 RS-2530-D185 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmRS-0321-D185 $310 RS-0521-D185 $320 RS-1021-D185 $350 RS-1521-D185 $375 RS-2521-D185 $405

PROTO 300 Analytical Columns - Stainless SteelPROTO 300 C4 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmRS-0346-W045 $310 RS-0546-W045 $320 RS-1046-W045 $350 RS-1546-W045 $375 RS-2546-W045 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmRS-0340-W045 $310 RS-0540-W045 $320 RS-1040-W045 $350 RS-1540-W045 $375 RS-2540-W045 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmRS-0330-W045 $310 RS-0530-W045 $320 RS-1030-W045 $350 RS-1530-W045 $375 RS-2530-W045 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmRS-0321-W045 $310 RS-0521-W045 $320 RS-1021-W045 $350 RS-1521-W045 $375 RS-2521-W045 $405

PROTO 300 C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmRS-0346-W185 $310 RS-0546-W185 $320 RS-1046-W185 $350 RS-1546-W185 $375 RS-2546-W185 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmRS-0340-W185 $310 RS-0540-W185 $320 RS-1040-W185 $350 RS-1540-W185 $375 RS-2540-W185 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmRS-0330-W185 $310 RS-0530-W185 $320 RS-1030-W185 $350 RS-1530-W185 $375 RS-2530-W185 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmRS-0321-W185 $310 RS-0521-W185 $320 RS-1021-W185 $350 RS-1521-W185 $375 RS-2521-W185 $405

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200

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PHALANX Analytical Columns - Stainless SteelPHALANX C18 3µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmPS-0346-C183 $380 PS-0546-C183 $390 PS-1046-C183 $440 PS-1546-C183 $475 PS-2546-C183 $50030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmPS-0340-C183 $380 PS-0540-C183 $390 PS-1040-C183 $440 PS-1540-C183 $475 PS-2540-C183 $50030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmPS-0330-C183 $380 PS-0530-C183 $390 PS-1030-C183 $440 PS-1530-C183 $475 PS-2530-C183 $50030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmPS-0321-C183 $380 PS-0521-C183 $390 PS-1021-C183 $440 PS-1521-C183 $475 PS-2521-C183 $500

PHALANX C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmPS-0346-C185 $310 PS-0546-C185 $320 PS-1046-C185 $350 PS-1546-C185 $375 PS-2546-C185 $40530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmPS-0340-C185 $310 PS-0540-C185 $320 PS-1040-C185 $350 PS-1540-C185 $375 PS-2540-C185 $40530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmPS-0330-C185 $310 PS-0530-C185 $320 PS-1030-C185 $350 PS-1530-C185 $375 PS-2530-C185 $40530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmPS-0321-C185 $310 PS-0521-C185 $320 PS-1021-C185 $350 PS-1521-C185 $375 PS-2521-C185 $405

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NEW 3µm

HPLC Columns (continued)Wide Range of Stainless Steel HPLC COLUMNS

Comparison of PHALANX to Kromasil C18 for a Pharmaceutical Drug Analysis150 X 4.6mm Columns, 1mL/min Linear Gradient for 50 minutes

Higgins Analytical thanks Amos Heckendorf of The Nest Group (www.nestgrp.com) for these data.

PHALANX Full Scale Chromatogram KROMASIL C18 Full Scale Chromatogram

PHALANX Expanded Scale Chromatogram KROMASIL C18 Expanded Scale Chromatogram

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See pages 62 - 63 for information on the wide variety of Guard Cartridges available for these Analytical Columns

TARGA Analytical Columns - Stainless SteelTARGA C8 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns

30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmTS-0346-C085 $290 TS-0546-C085 $300 TS-1046-C085 $320 TS-1546-C085 $350 TS-2546-C085 $37030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmTS-0340-C085 $290 TS-0540-C085 $300 TS-1040-C085 $320 TS-1540-C085 $350 TS-2540-C085 $37030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmTS-0330-C085 $290 TS-0530-C085 $300 TS-1030-C085 $320 TS-1530-C085 $350 TS-2530-C085 $37030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmTS-0321-C085 $290 TS-0521-C085 $300 TS-1021-C085 $320 TS-1521-C085 $350 TS-2521-C085 $370

TARGA C18 3µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmTS-0346-C183 $365 TS-0546-C183 $375 TS-1046-C183 $400 TS-1546-C183 $430 TS-2546-C183 $45530x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmTS-0340-C183 $365 TS-0540-C183 $375 TS-1040-C183 $400 TS-1540-C183 $430 TS-2540-C183 $45530x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmTS-0330-C183 $365 TS-0530-C183 $375 TS-1030-C183 $400 TS-1530-C183 $430 TS-2530-C183 $45530x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmTS-0321-C183 $365 TS-0521-C183 $375 TS-1021-C183 $400 TS-1521-C183 $430 TS-2521-C183 $455

TARGA C18 5µm 4.6, 4.0, 3.0, and 2.1mm I.D. s.s. columns30x4.6mm 50x4.6mm 100x4.6mm 150x4.6mm 250x4.6mmTS-0346-C185 $290 TS-0546-C185 $300 TS-1046-C185 $320 TS-1546-C185 $350 TS-2546-C185 $37030x4.0mm 50x4.0mm 100x4.0mm 150x4.0mm 250x4.0mmTS-0340-C185 $290 TS-0540-C185 $300 TS-1040-C185 $320 TS-1540-C185 $350 TS-2540-C185 $37030x3.0mm 50x3.0mm 100x3.0mm 150x3.0mm 250x3.0mmTS-0330-C185 $290 TS-0530-C185 $300 TS-1030-C185 $320 TS-1530-C185 $350 TS-2530-C185 $37030x2.1mm 50x2.1mm 100x2.1mm 150x2.1mm 250x2.1mmTS-0321-C185 $290 TS-0521-C185 $300 TS-1021-C185 $320 TS-1521-C185 $350 TS-2521-C185 $370

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CLIPEUS Microbore ColumnsCLIPEUS C8 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmCS-0301-C085 $310 CS-0501-C085 $320 CS-1001-C085 $350 CS-1501-C085 $375 CS-2501-C085 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmCS-03M8-C085 $310 CS-05M8-C085 $320 CS-10M8-C085 $350 CS-15M8-C085 $375 CS-25M8-C085 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmCS-03M5-C085 $310 CS-05M5-C085 $320 CS-10M5-C085 $350 CS-15M5-C085 $375 CS-25M5-C085 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmCS-03M3-C085 $310 CS-05M3-C085 $320 CS-10M3-C085 $350 CS-15M3-C085 $375 CS-25M3-C085 $405

CLIPEUS C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmCS-0301-C185 $310 CS-0501-C185 $320 CS-1001-C185 $350 CS-1501-C185 $375 CS-2501-C185 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmCS-03M8-C185 $310 CS-05M8-C185 $320 CS-10M8-C185 $350 CS-15M8-C185 $375 CS-25M8-C185 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmCS-03M5-C185 $310 CS-05M5-C185 $320 CS-10M5-C185 $350 CS-15M5-C185 $375 CS-25M5-C185 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmCS-03M3-C185 $310 CS-05M3-C185 $320 CS-10M3-C185 $350 CS-15M3-C185 $375 CS-25M3-C185 $405

CLIPEUS Cyano 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmCS-0301-CNP5 $325 CS-0501-CNP5 $340 CS-1001-CNP5 $365 CS-1501-CNP5 $390 CS-2501-CNP5 $42530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmCS-03M8-CNP5 $325 CS-05M8-CNP5 $340 CS-10M8-CNP5 $365 CS-15M8-CNP5 $390 CS-25M8-CNP5 $42530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmCS-03M5-CNP5 $325 CS-05M5-CNP5 $340 CS-10M5-CNP5 $365 CS-15M5-CNP5 $390 CS-25M5-CNP5 $42530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmCS-03M3-CNP5 $325 CS-05M3-CNP5 $340 CS-10M3-CNP5 $365 CS-15M3-CNP5 $390 CS-25M3-CNP5 $425

CLIPEUS Phenyl 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmCS-0301-PHN5 $325 CS-0501-PHN5 $340 CS-1001-PHN5 $365 CS-1501-PHN5 $390 CS-2501-PHN5 $42530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmCS-03M8-PHN5 $325 CS-05M8-PHN5 $340 CS-10M8-PHN5 $365 CS-15M8-PHN5 $390 CS-25M8-PHN5 $42530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmCS-03M5-PHN5 $325 CS-05M5-PHN5 $340 CS-10M5-PHN5 $365 CS-15M5-PHN5 $390 CS-25M5-PHN5 $42530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmCS-03M3-PHN5 $325 CS-05M3-PHN5 $340 CS-10M3-PHN5 $365 CS-15M3-PHN5 $390 CS-25M3-PHN5 $425

MICROBORE HPLC COLUMN ORDER ING INFORMAT ION

Microbore HPLC ColumnsWide Range of 1mm, 0.8mm, 0.5mm, and 0.3mm Columns

Widest Range of Microbore columnsizes with a wide range of sorbentsHiggins Analytical manufacturers thewidest range of Microbore columnformats available anywhere. In addition tothe glass-lined columns ranging from300µm to 1mm I.D., we also porvideunique PEEK/Titanium microborecolumns for specialized applications (seePiccolo).

Guide to MICROBORE Part NumbersxS-xx01-xxxx 1.0mm MicroborexS-xxM8-xxxx 0.8mm MicroborexS-xxM5-xxxx 0.5mm MicroborexS-xxM3-xxxx 0.3mm MicroboreSee Page 23 for complete Part Number information

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CLIPEUS Microbore Columns (cont.)CLIPEUS Silica 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmCS-0301-SIL5 $310 CS-0501-SIL5 $320 CS-1001-SIL5 $350 CS-1501-SIL5 $375 CS-2501-SIL5 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmCS-03M8-SIL5 $310 CS-05M8-SIL5 $320 CS-10M8-SIL5 $350 CS-15M8-SIL5 $375 CS-25M8-SIL5 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmCS-03M5-SIL5 $310 CS-05M5-SIL5 $320 CS-10M5-SIL5 $350 CS-15M5-SIL5 $375 CS-25M5-SIL5 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmCS-03M3-SIL5 $310 CS-05M3-SIL5 $320 CS-10M3-SIL5 $350 CS-15M3-SIL5 $375 CS-25M3-SIL5 $405

HAISIL100 Microbore ColumnsHAISIL 100 C8 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmHS-0301-M085 $310 HS-0501-M085 $320 HS-1001-M085 $350 HS-1501-M085 $375 HS-2501-M085 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmHS-03M8-M085 $310 HS-05M8-M085 $320 HS-10M8-M085 $350 HS-15M8-M085 $375 HS-25M8-M085 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmHS-03M5-M085 $310 HS-05M5-M085 $320 HS-10M5-M085 $350 HS-15M5-M085 $375 HS-25M5-M085 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmHS-03M3-M085 $310 HS-05M3-M085 $320 HS-10M3-M085 $350 HS-15M3-M085 $375 HS-25M3-M085 $405

HAISIL 100 C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmHS-0301-M185 $310 HS-0501-M185 $320 HS-1001-M185 $350 HS-1501-M185 $375 HS-2501-M185 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmHS-03M8-M185 $310 HS-05M8-M185 $320 HS-10M8-M185 $350 HS-15M8-M185 $375 HS-25M8-M185 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmHS-03M5-M185 $310 HS-05M5-M185 $320 HS-10M5-M185 $350 HS-15M5-M185 $375 HS-25M5-M185 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmHS-03M3-M185 $310 HS-05M3-M185 $320 HS-10M3-M185 $350 HS-15M3-M185 $375 HS-25M3-M185 $405

HAISIL 100 Silica 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmHS-0301-SIL5 $310 HS-0501-SIL5 $320 HS-1001-SIL5 $350 HS-1501-SIL5 $375 HS-2501-SIL5 $40530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmHS-03M8-SIL5 $310 HS-05M8-SIL5 $320 HS-10M8-SIL5 $350 HS-15M8-SIL5 $375 HS-25M8-SIL5 $40530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmHS-03M5-SIL5 $310 HS-05M5-SIL5 $320 HS-10M5-SIL5 $350 HS-15M5-SIL5 $375 HS-25M5-SIL5 $40530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmHS-03M3-SIL5 $310 HS-05M3-SIL5 $320 HS-10M3-SIL5 $350 HS-15M3-SIL5 $375 HS-25M3-SIL5 $405

MICROBORE HPLC COLUMN ORDER ING INFORMAT ION

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HAISIL300 Microbore ColumnsHAISIL 300 C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmHS-0301-W185 $320 HS-0501-W185 $330 HS-1001-W185 $350 HS-1501-W185 $375 HS-2501-W185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmHS-03M8-W185 $320 HS-05M8-W185 $330 HS-10M8-W185 $350 HS-15M8-W185 $375 HS-25M8-W185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmHS-03M5-W185 $320 HS-05M5-W185 $330 HS-10M5-W185 $350 HS-15M5-W185 $375 HS-25M5-W185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmHS-03M3-W185 $320 HS-05M3-W185 $330 HS-10M3-W185 $350 HS-15M3-W185 $375 HS-25M3-W185 $400

HAISIL HL Microbore ColumnsHAISIL HL C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmLS-0301-C185 $320 LS-0501-C185 $330 LS-1001-C185 $350 LS-1501-C185 $375 LS-2501-C185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmLS-03M8-C185 $320 LS-05M8-C185 $330 LS-10M8-C185 $350 LS-15M8-C185 $375 LS-25M8-C185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmLS-03M5-C185 $320 LS-05M5-C185 $330 LS-10M5-C185 $350 LS-15M5-C185 $375 LS-25M5-C185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmLS-03M3-C185 $320 LS-05M3-C185 $330 LS-10M3-C185 $350 LS-15M3-C185 $375 LS-25M3-C185 $400

HEAVY Microbore Columns HEAVY C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmVS-0301-C185 $320 VS-0501-C185 $330 VS-1001-C185 $350 VS-1501-C185 $375 VS-2501-C185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmVS-03M8-C185 $320 VS-05M8-C185 $330 VS-10M8-C185 $350 VS-15M8-C185 $375 VS-25M8-C185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmVS-03M5-C185 $320 VS-05M5-C185 $330 VS-10M5-C185 $350 VS-15M5-C185 $375 VS-25M5-C185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmVS-03M3-C185 $320 VS-05M3-C185 $330 VS-10M3-C185 $350 VS-15M3-C185 $375 VS-25M3-C185 $400

Microbore HPLC Columns (continued)Wide Range of 1mm, 0.5mm, and 0.3mm Columns

MICROBORE HPLC COLUMN ORDER ING INFORMAT ION

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33

PROTO 200 Microbore ColumnsPROTO 200 C4 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmRS-0301-D045 $320 RS-0501-D045 $330 RS-1001-D045 $350 RS-1501-D045 $375 RS-2501-D045 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmRS-03M8-D185 $320 RS-05M8-D185 $330 RS-10M8-D185 $350 RS-15M8-D185 $375 RS-25M8-D185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmRS-03M5-D045 $320 RS-05M5-D045 $330 RS-10M5-D045 $350 RS-15M5-D045 $375 RS-25M5-D045 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmRS-03M3-D045 $320 RS-05M3-D045 $330 RS-10M3-D045 $350 RS-15M3-D045 $375 RS-25M3-D045 $400

PROTO 200 C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmRS-0301-D185 $320 RS-0501-D185 $330 RS-1001-D185 $350 RS-1501-D185 $375 RS-2501-D185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmRS-03M8-D185 $320 RS-05M8-D185 $330 RS-10M8-D185 $350 RS-15M8-D185 $375 RS-25M8-D185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmRS-03M5-D185 $320 RS-05M5-D185 $330 RS-10M5-D185 $350 RS-15M5-D185 $375 RS-25M5-D185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmRS-03M3-D185 $320 RS-05M3-D185 $330 RS-10M3-D185 $350 RS-15M3-D185 $375 RS-25M3-D185 $400

PROTO 300 Microbore ColumnsPROTO 300 C4 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmRS-0301-W045 $320 RS-0501-W045 $330 RS-1001-W045 $350 RS-1501-W045 $375 RS-2501-W045 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmRS-03M8-W045 $320 RS-05M8-W045 $330 RS-10M8-W045 $350 RS-15M8-W045 $375 RS-25M8-W045 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmRS-03M5-W045 $320 RS-05M5-W045 $330 RS-10M5-W045 $350 RS-15M5-W045 $375 RS-25M5-W045 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmRS-03M3-W045 $320 RS-05M3-W045 $330 RS-10M3-W045 $350 RS-15M3-W045 $375 RS-25M3-W045 $400

PROTO 300 C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmRS-0301-W185 $320 RS-0501-W185 $330 RS-1001-W185 $350 RS-1501-W185 $375 RS-2501-W185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmRS-03M8-W185 $320 RS-05M8-W185 $330 RS-10M8-W185 $350 RS-15M8-W185 $375 RS-25M8-W185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmRS-03M5-W185 $320 RS-05M5-W185 $330 RS-10M5-W185 $350 RS-15M5-W185 $375 RS-25M5-W185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmRS-03M3-W185 $320 RS-05M3-W185 $330 RS-10M3-W185 $350 RS-15M3-W185 $375 RS-25M3-W185 $400

PHALANX Microbore Columns (5µm PHALANX on following page)PHALANX C18 3µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmPS-0301-C183 $380 PS-0501-C183 $390 PS-1001-C183 $440 PS-1501-C183 $475 PS-2501-C183 $50030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmPS-03M8-C183 $380 PS-05M8-C183 $390 PS-10M8-C183 $440 PS-15M8-C183 $475 PS-25M8-C183 $50030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmPS-03M5-C183 $380 PS-05M5-C183 $390 PS-10M5-C183 $440 PS-15M5-C183 $475 PS-25M5-C183 $50030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmPS-03M3-C183 $380 PS-05M3-C183 $390 PS-10M3-C183 $440 PS-15M3-C183 $475 PS-25M3-C183 $500

MICROBORE HPLC COLUMN ORDER ING INFORMAT ION

NEW 3µm

200

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PHALANX Microbore Columns (cont. - 3µm PHALANX on previous page)PHALANX C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmPS-0301-C185 $320 PS-0501-C185 $330 PS-1001-C185 $350 PS-1501-C185 $375 PS-2501-C185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmPS-03M8-C185 $320 PS-05M8-C185 $330 PS-10M8-C185 $350 PS-15M8-C185 $375 PS-25M8-C185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmPS-03M5-C185 $320 PS-05M5-C185 $330 PS-10M5-C185 $350 PS-15M5-C185 $375 PS-25M5-C185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmPS-03M3-C185 $320 PS-05M3-C185 $330 PS-10M3-C185 $350 PS-15M3-C185 $375 PS-25M3-C185 $400

TARGA Microbore Columns TARGA C8 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns

30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmTS-0301-C085 $320 TS-0501-C085 $330 TS-1001-C085 $350 TS-1501-C085 $375 TS-2501-C085 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmTS-03M8-C085 $320 TS-05M8-C085 $330 TS-10M8-C085 $350 TS-15M8-C085 $375 TS-25M8-C085 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmTS-03M5-C085 $320 TS-05M5-C085 $330 TS-10M5-C085 $350 TS-15M5-C085 $375 TS-25M5-C085 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmTS-03M3-C085 $320 TS-05M3-C085 $330 TS-10M3-C085 $350 TS-15M3-C085 $375 TS-25M3-C085 $400

TARGA C18 3µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmTS-0301-C183 $370 TS-0501-C183 $380 TS-1001-C183 $400 TS-1501-C183 $430 TS-2501-C183 $45530x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmTS-03M8-C183 $370 TS-05M8-C183 $380 TS-10M8-C183 $400 TS-15M8-C183 $430 TS-25M8-C183 $45530x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmTS-03M5-C183 $370 TS-05M5-C183 $380 TS-10M5-C183 $400 TS-15M5-C183 $430 TS-25M5-C183 $45530x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmTS-03M3-C183 $370 TS-05M3-C183 $380 TS-10M3-C183 $400 TS-15M3-C183 $430 TS-25M3-C183 $455

TARGA C18 5µm 1.0, 0.8, 0.5, and 0.3mm I.D. s.s. columns30x1.0mm 50x1.0mm 100x1.0mm 150x1.0mm 250x1.0mmTS-0301-C185 $320 TS-0501-C185 $330 TS-1001-C185 $350 TS-1501-C185 $375 TS-2501-C185 $40030x0.8mm 50x0.8mm 100x0.8mm 150x0.8mm 250x0.8mmTS-03M8-C185 $320 TS-05M8-C185 $330 TS-10M8-C185 $350 TS-15M8-C185 $375 TS-25M8-C185 $40030x0.5mm 50x0.5mm 100x0.5mm 150x0.5mm 250x0.5mmTS-03M5-C185 $320 TS-05M5-C185 $330 TS-10M5-C185 $350 TS-15M5-C185 $375 TS-25M5-C185 $40030x0.3mm 50x0.3mm 100x0.3mm 150x0.3mm 250x0.3mmTS-03M3-C185 $320 TS-05M3-C185 $330 TS-10M3-C185 $350 TS-15M3-C185 $375 TS-25M3-C185 $400

Microbore HPLC Columns (continued)Wide Range of 1mm, 0.5mm, and 0.3mm Columns

MICROBORE HPLC COLUMN ORDER ING INFORMAT ION

Higgins Analytical manufacturers the widest range of Microbore HPLC Columns in the world.

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35

MICROBORE POLYMER IC COLUMN ORDER ING INFORMAT ION

Comparison of a Higgins to Another Manufacturer’s 1mm Column

Vydac C18 238MS5115150 x 1.0mm

PROTO 200 C18150 x 1.0mm

Tryptic Digest of Carbamidomethylated BSA (see page 15 for details)

Page 38: HAI Price List 2005 - HPLC Column Manufacturer

S EM I - PREP AND PREP COLUMNS ORDER ING INFORMAT ION

36

CLIPEUS 10mm Semi-Prep and 20mm Prep ColumnsCLIPEUS C8 5µm 10 and 20mm I.D. s.s. columns

50x10mm 100x10mm 150x10mm 250x10mmCS-0510-C085 $525 CS-1010-C085 $700 CS-1510-C085 $980 CS-2510-C085 $119050x20mm 100x20mm 150x20mm 250x20mmCS-0520-C085 $1200 CS-1020-C085 $1715 CS-1520-C085 $2250 CS-2520-C085 $3300

CLIPEUS C18 10µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmCS-0510-C181 $320 CS-1010-C181 $395 CS-1510-C181 $565 CS-2510-C181 $71550x20mm 100x20mm 150x20mm 250x20mmCS-0520-C181 $950 CS-1020-C181 $1220 CS-1520-C181 $1560 CS-2520-C181 $1990

CLIPEUS C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmCS-0510-C185 $525 CS-1010-C185 $700 CS-1510-C185 $980 CS-2510-C185 $119050x20mm 100x20mm 150x20mm 250x20mmCS-0520-C185 $1200 CS-1020-C185 $1715 CS-1520-C185 $2250 CS-2520-C185 $3300

CLIPEUS Silica 10µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmCS-0510-SIL1 $300 CS-1010-SIL1 $370 CS-1510-SIL1 $530 CS-2510-SIL1 $67050x20mm 100x20mm 150x20mm 250x20mmCS-0520-SIL1 $890 CS-1020-SIL1 $1140 CS-1520-SIL1 $1460 CS-2520-SIL1 $1850

Semi-Prep (10mm) and Prep (20mm)ColumnsNot only does Higgins Analytical manufacturersemi-prep and prep columns with our mostpopular phases, we also produce guardcartridges for these columns. See pages 56 - 57 (10mm) and 60 - 61(20mm) for more information about guardcartridges, and pages 62 - 63 for cartridgeholders.

Guide to Semi-Prep and Prep Column Part NumbersxS-xx10-xxxx 10mm I.D. columnsxS-xx20-xxxx 20mm I.D. columnsSee Page 23 for complete Part Number information

Semi-Prep and Prep ColumnsWide Range of 10mm and 20mm i.d. columns

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37

HAISIL100 10mm Semi-Prep and 20mm Prep ColumnsHAISIL 100 C8 5µm 10 and 20mm I.D. s.s. columns

50x10mm 100x10mm 150x10mm 250x10mmHS-0510-M085 $525 HS-1010-M085 $700 HS-1510-M085 $980 HS-2510-M085 $119050x20mm 100x20mm 150x20mm 250x20mmHS-0520-M085 $1200 HS-1020-M085 $1715 HS-1520-M085 $2250 HS-2520-M085 $3300

HAISIL 100 C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmHS-0510-M185 $525 HS-1010-M185 $700 HS-1510-M185 $980 HS-2510-M185 $119050x20mm 100x20mm 150x20mm 250x20mmHS-0520-M185 $1200 HS-1020-M185 $1715 HS-1520-M185 $2250 HS-2520-M185 $3300

PHALANX 10mm Semi-Prep and 20mm Prep ColumnsPHALANX C18 10µm 10 and 20mm I.D. s.s. columns

50x10mm 100x10mm 150x10mm 250x10mmPS-0510-C181 $320 PS-1010-C181 $395 PS-1510-C181 $565 PS-2510-C181 $71550x20mm 100x20mm 150x20mm 250x20mmPS-0520-C181 $950 PS-1020-C181 $1220 PS-1520-C181 $1560 PS-2520-C181 $1990

PHALANX C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmPS-0510-C185 $545 PS-1010-C185 $700 PS-1510-C185 $980 PS-2510-C185 $119050x20mm 100x20mm 150x20mm 250x20mmPS-0520-C185 $1200 PS-1020-C185 $1715 PS-1520-C185 $2250 PS-2520-C185 $3300

PROTO 200 10mm Semi-Prep and 20mm Prep ColumnsPROTO 200 C4 10µm 10 and 20mm I.D. s.s. columns

50x10mm 100x10mm 150x10mm 250x10mmRS-0510-D041 $320 RS-1010-D041 $395 RS-1510-D041 $565 RS-2510-D041 $71550x20mm 100x20mm 150x20mm 250x20mmRS-0520-D041 $950 RS-1020-D041 $1220 RS-1520-D041 $1560 RS-2520-D041 $1990

PROTO 200 C4 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-D045 $525 RS-1010-D045 $700 RS-1510-D045 $980 RS-2510-D045 $119050x20mm 100x20mm 150x20mm 250x20mmRS-0520-D045 $1200 RS-1020-D045 $1715 RS-1520-D045 $2250 RS-2520-D045 $3300

PROTO 200 C18 10µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-D181 $320 RS-1010-D181 $395 RS-1510-D181 $565 RS-2510-D181 $71550x20mm 100x20mm 150x20mm 250x20mmRS-0520-D181 $950 RS-1020-D181 $1220 RS-1520-D181 $1560 RS-2520-D181 $1990

PROTO 200 C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-D185 $525 RS-1010-D185 $700 RS-1510-D185 $980 RS-2510-D185 $119050x20mm 100x20mm 150x20mm 250x20mmRS-0520-D185 $1200 RS-1020-D185 $1715 RS-1520-D185 $2250 RS-2520-D185 $3300

200

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PROTO 300 10mm Semi-Prep and 20mm Prep ColumnsPROTO 300 C4 10µm 10 and 20mm I.D. s.s. columns

50x10mm 100x10mm 150x10mm 250x10mmRS-0510-W041 $320 RS-1010-W041 $395 RS-1510-W041 $565 RS-2510-W041 $71550x20mm 100x20mm 150x20mm 250x20mmRS-0520-W041 $950 RS-1020-W041 $1220 RS-1520-W041 $1560 RS-2520-W041 $1990

PROTO 300 C4 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-W045 $525 RS-1010-W045 $700 RS-1510-W045 $980 RS-2510-W045 $119050x20mm 100x20mm 150x20mm 250x20mmRS-0520-W045 $1200 RS-1020-W045 $1715 RS-1520-W045 $2250 RS-2520-W045 $3300

PROTO 300 C18 10µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-W181 $320 RS-1010-W181 $395 RS-1510-W181 $565 RS-2510-W181 $71550x20mm 100x20mm 150x20mm 250x20mmRS-0520-W181 $950 RS-1020-W181 $1220 RS-1520-W181 $1560 RS-2520-W181 $1990

PROTO 300 C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmRS-0510-W185 $525 RS-1010-W185 $700 RS-1510-W185 $980 RS-2510-W185 $119050x20mm 100x20mm 150x20mm 250x20mmRS-0520-W185 $1200 RS-1020-W185 $1715 RS-1520-W185 $2250 RS-2520-W185 $3300

TARGA 10mm Semi-Prep and 20mm Prep ColumnsTARGA C18 10µm 10 and 20mm I.D. s.s. columns

50x10mm 100x10mm 150x10mm 250x10mmTS-0510-C181 $320 TS-1010-C181 $395 TS-1510-C181 $565 TS-2510-C181 $71550x20mm 100x20mm 150x20mm 250x20mmTS-0520-C181 $950 TS-1020-C181 $1220 TS-1520-C181 $1560 TS-2520-C181 $1990

TARGA C18 5µm 10 and 20mm I.D. s.s. columns50x10mm 100x10mm 150x10mm 250x10mmTS-0510-C185 $525 TS-1010-C185 $700 TS-1510-C185 $980 TS-2510-C185 $119050x20mm 100x20mm 150x20mm 250x20mmTS-0520-C185 $1200 TS-1020-C185 $1715 TS-1520-C185 $2250 TS-2520-C185 $3300

See pages 56 - 57 and 60 - 61 for information about Guard Cartridges available for these Prep and Semi-Prep Columns

38

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Prep Scale (20mm)

Prep Scale (20mm)

Semi-Prep Scale (10mm)

See pages 66 - 67 for information about the Prep and Semi-Prep Cartridge Holders

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Robust design with HighPerformance:To the right, aBSA tryptic digest on a150cm x 75µm Targa C18 5µm Caπllary.P/N TC-2507-C185 cut to 15cm.

CAπ L LARY ORDER ING INFORMAT ION

CaπllaryCAPILLARY HPLC COLUMNS

A New Benchmark in Capillary ColumnQuality and Cost:Higgins Analytical’s unique approach pro-vides a flexible format and reasonablypriced capillary HPLC column for LC-MSapplications. Caπllary, π, columns are allmanufactured and tested as 250mmproducts and are offered for the reason-able price of $314.16 for both 75µm and150µm versions.

Cutting and trimming a Caπllary™ Column:The Caπllaries can be used as is, or theycan be easily cut to any required length,with a ceramic cutter that is includedwith each column. Caπllary™ columnsare nominally 25cm in length. Unless thecapillary is going to be used in its fulllength, the white ceramic cutter is usedto cut the column to the required size.Measure from the frit in the center ofthe green section. This technique can alsobe useful for removing a short sectionfrom the column inlet if it becomes con-taminated or plugged. Similarly, the col-umn outlet can be trimmed to a properlength before attaching it to the post-col-umn device.

Plumbing Considerations:The Caπllary™ 75 and Caπllary™ 150columns have standard 360µm outerdiameters which can be convenientlyplumbed to HPLC systems in a widevariety of manners. The optional HigginsMicro Column Connection Kit (P/N MIC-KIT,see page 74) includes sleeves and finger-tight bushings to facilitate capillary col-umn attachment to most injection valves,detectors, and MS source inlets.

π

TM

NEW

$314.16

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HAISIL 300 C18 5µm250x0.075mmHC-2507-W185 $314.16250x0.150mmHC-2515-W185 $314.16

HAISIL HL C18 5µm250x0.075mmLC-2507-C185 $314.16250x0.150mmLC-2515-C185 $314.16

HEAVY C18 5µm250x0.075mmVC-2507-C185 $314.16250x0.150mmVC-2515-C185 $314.16

PROTO 200 C4 5µm250x0.075mmRC-2507-D045 $314.16250x0.150mm RC-2515-D045 $314.16

PROTO 200 C18 5µm250x0.075mmRC-2507-D185 $314.16250x0.150mmRC-2515-D185 $314.16

PROTO 300 C4 5µm75µm and 150µm 250x0.075mmRC-2507-W045 $314.16250x0.150mmRC-2515-W045 $314.16

PROTO 300 C18 5µm75µm and 150µm 250x0.075mmRC-2507-W185 $314.16250x0.150mmRC-2515-W185 $314.16

PHALANX C18 5µm75µm and 150µm 250x0.075mmPC-2507-C185 $314.16250x0.150mmPC-2515-C185 $314.16

TARGA C8 5µm75µm and 150µm250x0.075mmTC-2507-C085 $314.16250x0.150mmTC-2515-C085 $314.16

TARGA C18 5µm250x0.075mmTC-2507-C185 $314.16250x0.150mmTC-2515-C185 $314.16

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CAπ L LARY ORDER ING INFORMAT ION

200

Caπllary™ is a trademark of Higgins Analytical, Inc.

CLIPEUS C8 5µm250x0.075mmCC-2507-C085 $314.16250x0.150mmCC-2515-C085 $314.16

CLIPEUS C18 5µm250x0.075mmCC-2507-C185 $314.16250x0.150mmCC-2515-C185 $314.16

CLIPEUS CYANO 5µm250x0.075mmCC-2507-CNP5 $314.16250x0.150mmCC-2515-CNP5 $314.16

CLIPEUS PHENYL 5µm250x0.075mmCC-2507-PHN5 $314.16250x0.150mmCC-2515-PHN5 $314.16

CLIPEUS SILICA 5µm250x0.075mmCC-2507-SIL5 $314.16250x0.150mmCC-2515-SIL5 $314.16

HAISIL 100 C8 5µm250x0.075mmHC-2507-M085 $314.16250x0.150mmHC-2515-M085 $314.16

HAISIL 100 C18 5µm250x0.075mmHC-2507-M185 $314.16250x0.150mmHC-2515-M185 $314.16

HAISIL 100 SILICA 5µm250x0.075mmHC-2507-SIL5 $314.16250x0.150mmHC-2515-SIL5 $314.16

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CAPELLINI 6-32CAPILLARY HPLC COLUMNS with 6-32 Thread PEEK/Ti Fittings

Higgins Analytical Capellini are robust,high performance capillary HPLCcolumns for LC-MS and other very smallscale applications. The inert PEEK andtitanium fittings complement the HigginsAnalytical sorbents such as TARGAwhich was developed to enable LC-MSapplications without TFA.

Our no nonsense testing proceduredoes not resort to sample and flowsplitting techniques, inaccurate k’ values,and miniscule chromatograms, as dosome manufacturers, to illustrate columnperformance. The CofA’s symmetricalpeaks and the high resolution map speakfor themselves.

Capellini - Back by Popular DemandWhen Higgins Analytical introducedCaπllary columns three years ago, weremoved Capellini from our catalog,however, we contnued to manufacturethis design for customers that had beenusing it for several years. Somewhat toour surprise, Capellini sales havecontinued to grow and we still receivenumerous product inquiries in spite oftheir absence from our literature. WhileCaπllary columns are very efficient andcost effective (see page 40), we havefound that many chromatographersprefer the symmetrical, flow reversabledesign and the 6-32 threaded end-fittingsof Capellini. Even though Capellini aremore expensive to manufacture andsomewhat less efficient than Caπllarycolumns, due to the end fitting design,they enjoy a wide variety of uses thatinvolve flow reversal, such trap applica-tions.

Guide to CAPELLINI Part NumbersxFS-xx07-xxxx 75µm CAPELLINI 6-32xFS-xx15-xxxx 150µm CAPELLINI 6-32See Page 23 for complete Part Number information

Capillary HPLC ColumnsUnique Features:• 6-32 threads for capillary fittings• PEEK/Titanium fittings• Tested and supplied with CofA• High performance• Higgins Analytical Sorbents

Applications:• LC-MS• Microbore HPLC

Standard Sizes:• 50 to 150 x 0.150mm• 50 to 150 x 0.075mm• Longer and shorter lengths available

High Selectivity:A partial 1.5µL/min LCMSMS map of aBSA tryptic digest.

CAPELL IN I 6 - 3 2 ORDER ING INFORMAT ION

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CLIPEUS Capillary Columns with 6-32 threadsCLIPEUS C8 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmCFS-0507-C085 $400 CFS-1007-C085 $400 CFS-1507-C085 $40050x0.150mm 100x0.150mm 150x0.150mmCFS-0515-C085 $400 CFS-1015-C085 $400 CFS-1515-C085 $400

CLIPEUS C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCFS-0507-C185 $400 CFS-1007-C185 $400 CFS-1507-C185 $40050x0.150mm 100x0.150mm 150x0.150mmCFS-0515-C185 $400 CFS-1015-C185 $400 CFS-1515-C185 $400

CLIPEUS Cyano 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCFS-0507-CNP5 $400 CFS-1007-CNP5 $400 CFS-1507-CNP5 $40050x0.150mm 100x0.150mm 150x0.150mmCFS-0515-CNP5 $400 CFS-1015-CNP5 $400 CFS-1515-CNP5 $400

CLIPEUS Phenyl 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCFS-0507-PHN5 $400 CFS-1007-PHN5 $400 CFS-1507-PHN5 $40050x0.150mm 100x0.150mm 150x0.150mmCFS-0515-PHN5 $400 CFS-1015-PHN5 $400 CFS-1515-PHN5 $400

CLIPEUS Silica 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCFS-0507-SIL5 $400 CFS-1007-SIL5 $400 CFS-1507-SIL5 $40050x0.150mm 100x0.150mm 150x0.150mmCFS-0515-SIL5 $400 CFS-1015-SIL5 $400 CFS-1515-SIL5 $400

HAISIL100 Capillary Columns with 6-32 threadsHAISIL 100 C8 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmHFS-0507-M085 $400 HFS-1007-M085 $400 HFS-1507-M085 $40050x0.150mm 100x0.150mm 150x0.150mmHFS-0515-M085 $400 HFS-1015-M085 $400 HFS-1515-M085 $400

HAISIL 100 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmHFS-0507-M185 $400 HFS-1007-M185 $400 HFS-1507-M185 $40050x0.150mm 100x0.150mm 150x0.150mmHFS-0515-M185 $400 HFS-1015-M185 $400 HFS-1515-M185 $400

HAISIL 100 Silica 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmHFS-0507-SIL5 $400 HFS-1007-SIL5 $400 HFS-1507-SIL5 $40050x0.150mm 100x0.150mm 150x0.150mmHFS-0515-SIL5 $400 HFS-1015-SIL5 $400 HFS-1515-SIL5 $400

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44

HAISIL300 Capillary Columns with 6-32 threadsHAISIL 300 C18 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmHFS-0507-W185 $400 HFS-1007-W185 $400 HFS-1507-W185 $40050x0.150mm 100x0.150mm 150x0.150mmHFS-0515-W185 $400 HFS-1015-W185 $400 HFS-1515-W185 $400

HAISIL HL Capillary Columns with 6-32 threadsHAISIL HL C18 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmLFS-0507-C185 $400 LFS-1007-C185 $400 LFS-1507-C185 $40050x0.150mm 100x0.150mm 150x0.150mmLFS-0515-C185 $400 LFS-1015-C185 $400 LFS-1515-C185 $400

HEAVY Capillary Columns with 6-32 threads HEAVY C18 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmVFS-0507-C185 $400 VFS-1007-C185 $400 VFS-1507-C185 $40050x0.150mm 100x0.150mm 150x0.150mmVFS-0515-C185 $400 VFS-1015-C185 $400 VFS-1515-C185 $400

PROTO 200 Capillary Columns with 6-32 threadsPROTO 200 C4 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmRFS-0507-D045 $400 RFS-1007-D045 $400 RFS-1507-D045 $40050x0.150mm 100x0.150mm 150x0.150mmRFS-0515-D045 $400 RFS-1015-D045 $400 RFS-1515-D045 $400

PROTO 200 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmRFS-0507-D185 $400 RFS-1007-D185 $400 RFS-1507-D185 $40050x0.150mm 100x0.150mm 150x0.150mmRFS-0515-D185 $400 RFS-1015-D185 $400 RFS-1515-D185 $400

CAPELLINI 6-32 (cont.)CAPILLARY HPLC COLUMNS with 6-32 Thread PEEK/Ti Fittings

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PROTO 300 Capillary Columns with 6-32 threadsPROTO 300 C4 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmRFS-0507-W045 $400 RFS-1007-W045 $400 RFS-1507-W045 $40050x0.150mm 100x0.150mm 150x0.150mmRFS-0515-W045 $400 RFS-1015-W045 $400 RFS-1515-W045 $400

PROTO 300 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmRFS-0507-W185 $400 RFS-1007-W185 $400 RFS-1507-W185 $40050x0.150mm 100x0.150mm 150x0.150mmRFS-0515-W185 $400 RFS-1015-W185 $400 RFS-1515-W185 $400

PHALANX Capillary Columns with 6-32 threadsPHALANX C18 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmPFS-0507-C185 $400 PFS-1007-C185 $400 PFS-1507-C185 $40050x0.150mm 100x0.150mm 150x0.150mmPFS-0515-C185 $400 PFS-1015-C185 $400 PFS-1515-C185 $400

TARGA Capillary Columns with 6-32 threadsTARGA C8 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmTFS-0507-C085 $400 TFS-1007-C085 $400 TFS-1507-C085 $40050x0.150mm 100x0.150mm 150x0.150mmTFS-0515-C085 $400 TFS-1015-C085 $400 TFS-1515-C085 $400

TARGA C18 3µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmTFS-0507-C183 $400 TFS-1007-C183 $400 TFS-1507-C183 $40050x0.150mm 100x0.150mm 150x0.150mmTFS-0515-C183 $400 TFS-1015-C183 $400 TFS-1515-C183 $400

TARGA C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmTFS-0507-C185 $400 TFS-1007-C185 $400 TFS-1507-C185 $40050x0.150mm 100x0.150mm 150x0.150mmTFS-0515-C185 $400 TFS-1015-C185 $400 TFS-1515-C185 $400

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CAPELLINI 10-32CAPILLARY HPLC COLUMNS with 10-32 Thread s.s. Fittings

Higgins Analytical Capellini have beenpopular with chromatographers requiringan easy-to-use and robust capillaryHPLC column. The original Capellini(now called Capellini 6-32) wereavailable only with 6-32 fittings. Now, wehave another version for chromatogra-phers prefering 10-32 thread fittings -Capellini 10-32. Another differencebetween the two versions of Capellini isthat the 10-32 threads are stainless steelrather than PEEK. Both types are madewith titanium frits on each end, providinginertness and flow direction reversability.

Guide to CAPELLINI 10-32 Part NumbersxPS-xx07-xxxx 75µm CAPELLINI 10-32xPS-xx15-xxxx 150µm CAPELLINI 10-32See Page 23 for complete Part Number information

Capillary HPLC ColumnsUnique Features:• 10-32 threads for standard fittings• s.s. End Fittings/Titanium Frits• Tested and supplied with CofA• High performance• Higgins Analytical Sorbents

Applications:• LC-MS• Microbore HPLC

Standard Sizes:• 50 to 150 x 0.150mm• 50 to 150 x 0.075mm• Longer and shorter lengths available

46

NEW

Robust design with HighPerformance:To the right, aBSA tryptic digest on a150cm x 75µm Targa C18 5µm Capellini.P/N TC-1507-C185

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47

CLIPEUS Capillary Columns with 10-32 threadsCLIPEUS C8 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmCPS-0507-C085 $450 CPS-1007-C085 $450 CPS-1507-C085 $45050x0.150mm 100x0.150mm 150x0.150mmCPS-0515-C085 $450 CPS-1015-C085 $450 CPS-1515-C085 $450

CLIPEUS C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCPS-0507-C185 $450 CPS-1007-C185 $450 CPS-1507-C185 $45050x0.150mm 100x0.150mm 150x0.150mmCPS-0515-C185 $450 CPS-1015-C185 $450 CPS-1515-C185 $450

CLIPEUS Cyano 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCPS-0507-CNP5 $450 CPS-1007-CNP5 $450 CPS-1507-CNP5 $45050x0.150mm 100x0.150mm 150x0.150mmCPS-0515-CNP5 $450 CPS-1015-CNP5 $450 CPS-1515-CNP5 $450

CLIPEUS Phenyl 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCPS-0507-PHN5 $450 CPS-1007-PHN5 $450 CPS-1507-PHN5 $45050x0.150mm 100x0.150mm 150x0.150mmCPS-0515-PHN5 $450 CPS-1015-PHN5 $450 CPS-1515-PHN5 $450

CLIPEUS Silica 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmCPS-0507-SIL5 $450 CPS-1007-SIL5 $450 CPS-1507-SIL5 $45050x0.150mm 100x0.150mm 150x0.150mmCPS-0515-SIL5 $450 CPS-1015-SIL5 $450 CPS-1515-SIL5 $450

HAISIL100 Capillary Columns with 10-32 threadsHAISIL 100 C8 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmHPS-0507-M085 $450 HPS-1007-M085 $450 HPS-1507-M085 $45050x0.150mm 100x0.150mm 150x0.150mmHPS-0515-M085 $450 HPS-1015-M085 $450 HPS-1515-M085 $450

HAISIL 100 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmHPS-0507-M185 $450 HPS-1007-M185 $450 HPS-1507-M185 $45050x0.150mm 100x0.150mm 150x0.150mmHPS-0515-M185 $450 HPS-1015-M185 $450 HPS-1515-M185 $450

HAISIL 100 Silica 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmHPS-0507-SIL5 $450 HPS-1007-SIL5 $450 HPS-1507-SIL5 $45050x0.150mm 100x0.150mm 150x0.150mmHPS-0515-SIL5 $450 HPS-1015-SIL5 $450 HPS-1515-SIL5 $450

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HAISIL300 Capillary Columns with 10-32 threadsHAISIL 300 C18 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmHPS-0507-W185 $450 HPS-1007-W185 $450 HPS-1507-W185 $45050x0.150mm 100x0.150mm 150x0.150mmHPS-0515-W185 $450 HPS-1015-W185 $450 HPS-1515-W185 $450

HAISIL HL Capillary Columns with 10-32 threadsHAISIL HL C18 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmLPS-0507-C185 $450 LPS-1007-C185 $450 LPS-1507-C185 $45050x0.150mm 100x0.150mm 150x0.150mmLPS-0515-C185 $450 LPS-1015-C185 $450 LPS-1515-C185 $450

HEAVY Capillary Columns with 10-32 threads HEAVY C18 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmVPS-0507-C185 $450 VPS-1007-C185 $450 VPS-1507-C185 $45050x0.150mm 100x0.150mm 150x0.150mmVPS-0515-C185 $450 VPS-1015-C185 $450 VPS-1515-C185 $450

PROTO 200 Capillary Columns with 10-32 threadsPROTO 200 C4 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmRPS-0507-D045 $450 RPS-1007-D045 $450 RPS-1507-D045 $45050x0.150mm 100x0.150mm 150x0.150mmRPS-0515-D045 $450 RPS-1015-D045 $450 RPS-1515-D045 $450

PROTO 200 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmRPS-0507-D185 $450 RPS-1007-D185 $450 RPS-1507-D185 $45050x0.150mm 100x0.150mm 150x0.150mmRPS-0515-D185 $450 RPS-1015-D185 $450 RPS-1515-D185 $450

CAPELLINI 10-32 (cont.)CAPILLARY HPLC COLUMNS with 10-32 Thread s.s. Fittings

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PROTO 300 Capillary Columns with 10-32 threadsPROTO 300 C4 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmRPS-0507-W045 $450 RPS-1007-W045 $450 RPS-1507-W045 $45050x0.150mm 100x0.150mm 150x0.150mmRPS-0515-W045 $450 RPS-1015-W045 $450 RPS-1515-W045 $450

PROTO 300 C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmRPS-0507-W185 $450 RPS-1007-W185 $450 RPS-1507-W185 $45050x0.150mm 100x0.150mm 150x0.150mmRPS-0515-W185 $450 RPS-1015-W185 $450 RPS-1515-W185 $450

PHALANX Capillary Columns with 10-32 threadsPHALANX C18 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmPPS-0507-C185 $450 PPS-1007-C185 $450 PPS-1507-C185 $45050x0.150mm 100x0.150mm 150x0.150mmPPS-0515-C185 $450 PPS-1015-C185 $450 PPS-1515-C185 $450

TARGA Capillary Columns with 10-32 threadsTARGA C8 5µm 75µm and 150µm Capillary columns

50x0.075mm 100x0.075mm 150x0.075mmTPS-0507-C085 $450 TPS-1007-C085 $450 TPS-1507-C085 $45050x0.150mm 100x0.150mm 150x0.150mmTPS-0515-C085 $450 TPS-1015-C085 $450 TPS-1515-C085 $450

TARGA C18 3µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmTPS-0507-C183 $450 TPS-1007-C183 $450 TPS-1507-C183 $45050x0.150mm 100x0.150mm 150x0.150mmTPS-0515-C183 $450 TPS-1015-C183 $450 TPS-1515-C183 $450

TARGA C18 5µm 75µm and 150µm Capillary columns 50x0.075mm 100x0.075mm 150x0.075mmTPS-0507-C185 $450 TPS-1007-C185 $450 TPS-1507-C185 $45050x0.150mm 100x0.150mm 150x0.150mmTPS-0515-C185 $450 TPS-1015-C185 $450 TPS-1515-C185 $450

NEW

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S PR ITE ORDER ING INFORMAT ION

SPRITEDIRECT CONNECT HPLC COLUMN

The Sprite family of narrowbore HPLCcolumns is a unique high performanceproduct for LC-MS and other high-speed, small volume applications. Allthree sizes of 2.1mm I.D Sprite HPLCcolumns are individually tested. ACertificate of Analysis accompanies eachcolumn. The Sprite’s certificate includesthe original chromatogram and compre-hensive testing and sorbentdocumentation.

The inert PEEK and titanium design hasbeen proven to be structurally sound toover 5,000 PSI (340Bar).

The optional Higgins Micro ColumnConnection Kit (P/N MIC-KIT, see page 74)includes sleeves and finger-tight bushingsto facilitate the attachment of a Sprite tomost injection valves, detectors, and MSsource inlets.

Guide to SPRITE Part NumbersxR-0121-xxxx 10 x 2.1mm SpritexR-0221-xxxx 20 x 2.1mm SpritexR-0421-xxxx 40 x 2.1mm SpriteSee Page 23 for complete Part Number information

CLIPEUS SpriteCLIPEUS C8 5µm

10X2.1mm 20X2.1mm 40X2.1mmCR-0121-C085 $175 CR-0221-C085 $185 CR-0421-C085 $215

CLIPEUS C18 5µm10X2.1mm 20X2.1mm 40X2.1mmCR-0121-C185 $175 CR-0221-C185 $185 CR-0421-C185 $215

CLIPEUS Cyano 5µm10X2.1mm 20X2.1mm 40X2.1mmCR-0121-CNP5 $175 CR-0221-CNP5 $185 CR-0421-CNP5 $215

CLIPEUS Phenyl 5µm10X2.1mm 20X2.1mm 40X2.1mmCR-0121-PHN5 $175 CR-0221-PHN5 $185 CR-0421-PHN5 $215

CLIPEUS Silica 5µm10X2.1mm 20X2.1mm 40X2.1mmCR-0121-SIL5 $175 CR-0221-SIL5 $185 CR-0421-SIL5 $215

HAISIL 100 SpriteHAISIL 100 C8 5µm

10X2.1mm 20X2.1mm 40X2.1mmHR-0121-M085 $175 HR-0221-M085 $185 HR-0421-M085 $215

HAISIL 100 C18 5µm10X2.1mm 20X2.1mm 40X2.1mmHR-0121-M185 $175 HR-0221-M185 $185 HR-0421-M185 $215

HAISIL 300 SpriteHAISIL 300 C18 5µm

10X2.1mm 20X2.1mm 40X2.1mmHR-0121-W185 $175 HR-0221-W185 $185 HR-0421-W185 $215

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S PR ITE ORDER ING INFORMAT ION

HAISIL HL SpriteHAISIL HL C18 5µm

10X2.1mm 20X2.1mm 40X2.1mmLR-0121-C185 $175 LR-0221-C185 $185 LR-0421-C185 $215

HEAVY SpriteHEAVY C18 5µm

10X2.1mm 20X2.1mm 40X2.1mmVR-0121-C185 $175 VR-0221-C185 $185 VR-0421-C185 $215

PHALANX SpritePHALANX C18 5µm

10X2.1mm 20X2.1mm 40X2.1mmPR-0121-C185 $175 PR-0221-C185 $185 PR-0421-C185 $215

PROTO 200 SpritePROTO 200 C4 5µm

10X2.1mm 20X2.1mm 40X2.1mmRR-0121-D045 $175 RR-0221-D045 $185 RR-0421-D045 $215

PROTO 200 C18 5µm10X2.1mm 20X2.1mm 40X2.1mmRR-0121-D185 $175 RR-0221-D185 $185 RR-0421-D185 $215

PROTO 300 SpritePROTO 300 C4 5µm

10X2.1mm 20X2.1mm 40X2.1mmRR-0121-W045 $175 RR-0221-W045 $185 RR-0421-W045 $215

PROTO 300 C18 5µm10X2.1mm 20X2.1mm 40X2.1mmRR-0121-W185 $175 RR-0221-W185 $185 RR-0421-W185 $215

TARGA SpriteTARGA C8 5µm

10X2.1mm 20X2.1mm 40X2.1mmTR-0121-C085 $175 TR-0221-C085 $185 TR-0421-C085 $215

TARGA C18 5µm10X2.1mm 20X2.1mm 40X2.1mmTR-0121-C185 $175 TR-0221-C185 $185 TR-0421-C185 $215

SPRITE is Designed to Operate inEither Flow DirectionA SPRITE is made of PEEK and titaniumand can be connected directly to asample injection valve or directly to amass spectrometer’s source inlet.

200

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The Piccolo family of microbore HPLCcolumns is a unique high performanceproduct for LC-MS and other high-speed, small volume applications. Theseefficient columns are the only productsof their type that are individually tested.A Certificate of Analysis accompanieseach column.

In addition to analytical microboreapplications Piccolo columns are idealguards for protecting longer microboreHPLC columns.

Piccolo column void volumes range fromapproximately 5µl to 300nL, makingthem perfect for a wide variety oftrapping, desalting, and trace enrichmentapplications. These small columns can beconveniently used with micro injectionand switching valves as pictured on theleft.

The optional Higgins Micro ColumnConnection Kit (P/N MIC-KIT, see page 74)includes sleeves and finger-tight bushingsto facilitate the attachment of a Piccoloto most injection valves, detectors, andMS source inlets.

PICCOLOSHORT MICROBORE TRAPS AND COLUMNS

Guide to PICCOLO Part NumbersxP-M2M3-xxxx 2.5 x 0.3mm PICCOLOxP-M2M5-xxxx 2.5 x 0.5mm PICCOLOxP-M501-xxxx 5 x 1mm PICCOLOxP-0101-xxxx 10 x 1mm PICCOLOxP-0301-xxxx 30 x 1mm PICCOLOSee Page 23 for complete Part Number information

CLIPEUS PiccoloCLIPEUS C8 5µm

2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmCP-M2M3-C085 $175 CP-M2M5-C085 $175 CP-M501-C085 $175 CP-0101-C085 $175 CP-0301-C085 $220

CLIPEUS C18 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmCP-M2M3-C185 $175 CP-M2M5-C185 $175 CP-M501-C185 $175 CP-0101-C185 $175 CP-0301-C185 $220

CLIPEUS Cyano 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmCP-M2M3-CNP5 $175 CP-M2M5-CNP5 $175 CP-M501-CNP5 $175 CP-0101-CNP5 $175 CP-0301-CNP5 $220

CLIPEUS Phenyl 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmCP-M2M3-PHN5 $175 CP-M2M5-PHN5 $175 CP-M501-PHN5 $175 CP-0101-PHN5 $175 CP-0301-PHN5 $220

CLIPEUS Silica 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmCP-M2M3-SIL5 $175 CP-M2M5-SIL5 $175 CP-M501-SIL5 $175 CP-0101-SIL5 $175 CP-0301-SIL5 $220

HAISIL 100 PiccoloHAISIL 100 C8 5µm

2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmHP-M2M3-M085 $175 HP-M2M5-M085 $175 HP-M501-M085 $175 HP-0101-M085 $175 HP-0301-M085 $220

HAISIL 100 C18 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmHP-M2M3-M185 $175 HP-M2M5-M185 $175 HP-M501-M185 $175 HP-0101-M185 $175 HP-0301-M185 $220

HAISIL 300 PiccoloHAISIL 300 C18 5µm

2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmHP-M2M3-W185 $175 HP-M2M5-W185 $175 HP-M501-W185 $175 HP-0101-W185 $175 HP-0301-W185 $220

P ICCOLO ORDER ING INFORMAT ION

NEW

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HAISIL HL PiccoloHAISIL HL C18 5µm

2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmLP-M2M3-C185 $175 LP-M2M5-C185 $175 LP-M501-C185 $175 LP-0101-C185 $175 LP-0301-C185 $220

HEAVY PiccoloHEAVY C18 5µm

2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmVP-M2M3-C185 $175 VP-M2M5-C185 $175 VP-M501-C185 $175 VP-0101-C185 $175 VP-0301-C185 $220

PHALANX PiccoloPHALANX C18 5µm

2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmPP-M2M3-C185 $175 PP-M2M5-C185 $175 PP-M501-C185 $175 PP-0101-C185 $175 PP-0301-C185 $220

PROTO 200 PiccoloPROTO 200 C4 5µm

2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmRP-M2M3-D045 $175 RP-M2M5-D045 $175 RP-M501-D045 $175 RP-0101-D045 $175 RP-0301-D045 $220

PROTO 200 C18 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmRP-M2M3-D185 $175 RP-M2M5-D185 $175 RP-M501-D185 $175 RP-0101-D185 $175 RP-0301-D185 $220

PROTO 300 PiccoloPROTO 300 C4 5µm

2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmRP-M2M3-W045 $175 RP-M2M5-W045 $175 RP-M501-W045 $175 RP-0101-W045 $175 RP-0301-WO45 $220

PROTO 300 C18 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmRP-M2M3-W185 $175 RP-M2M5-W185 $175 RP-M501-W185 $175 RP-0101-W185 $175 RP-0301-W185 $220

TARGA PiccoloTARGA C8 5µm

2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmTP-M2M3-C085 $175 TP-M2M5-C085 $175 TP-M501-C085 $175 TP-0101-C085 $175 TP-0301-C085 $220

TARGA C18 5µm2.5X0.3mm 2.5X0.5mm 5X1mm 10x1mm 30x1mmTP-M2M3-C185 $175 TP-M2M5-C185 $175 TP-M501-C185 $175 TP-0101-C185 $175 TP-0301-C185 $220

PICCOLO (continued)SHORT MICROBORE COLUMNS

P ICCOLO ORDER ING INFORMAT ION

200

NEW

30x1mm

For 3 tim

es greater

trapping capacity

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HAICART CARTRIDGESSTAINLESS STEEL CARTRIDGE COLUMNS

The HAICART family of HPLC cartridgecolumns provides a convenient andeconomical approach to guard column,trace enrichment, and fast analysisapplications. These stainless steelcartridges are available with 3.2mm and4.6mm internal diameters and with 2, 4,5, or 10cm lengths. This wide selectionmakes it possible to address numerousanalytical needs. See pages 66 - 67 for information aboutthe cartridge holders required forHiggins Analytical’s HAICART CartridgeColumns. HAICART cartridges are alsocompatible with PE/Brownlee MPLCcartridge holders.

Guide to HAICART Analytical Cartridge Part NumbersxF-0421-xxxx 40 x 3.2mm stainless steel cartridgexF-0532-xxxx 50 x 3.2mm stainless steel cartridgexF-1032-xxxx 100 x 3.2mm stainless steel cartridgexF-0446-xxxx 40 x 4.6mm stainless steel cartridgexF-0546-xxxx 50 x 4.6mm stainless steel cartridgexF-1046-xxxx 100 x 4.6mm stainless steel cartridgeSee Page 23 for complete Part Number information

HA ICART STA INLE S S STEE L CARTR IDGE COLUMNS

CLIPEUS 3.2 and 4.6mm I.D. S.S.CartridgesCLIPEUS C8 5µm

20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgCF-0232-C085 $175 CF-0432-C085 $290 CF-0532-C085 $305 CF-1032-C085 $205

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgCF-0446-C085 $290 CF-0546-C085 $305 CF-1046-C085 $205

CLIPEUS C18 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgCF-0232-C185 $175 CF-0432-C185 $290 CF-0532-C185 $305 CF-1032-C185 $205

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgCF-0446-C185 $290 CF-0546-C185 $305 CF-1046-C185 $205

CLIPEUS Cyano 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgCF-0232-CNP5 $185 CF-0432-CNP5 $305 CF-0532-CNP5 $320 CF-1032-CNP5 $215

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgCF-0446-CNP5 $305 CF-0546-CNP5 $320 CF-1046-CNP5 $215

CLIPEUS Phenyl 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgCF-0232-PHN5 $185 CF-0432-PHN5 $305 CF-0532-PHN5 $320 CF-1032-PHN5 $215

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgCF-0446-PHN5 $305 CF-0546-PHN5 $320 CF-1046-PHN5 $215

CLIPEUS Silica 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgCF-0232-SIL5 $175 CF-0432-SIL5 $290 CF-0532-SIL5 $305 CF-1032-SIL5 $205

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgCF-0446-SIL5 $290 CF-0546-SIL5 $305 CF-1046-SIL5 $205

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HAISIL 100 C8 3.2 and 4.6mm I.D. S.S.CartridgesHAISIL 100 C8 5µm

20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgHF-0232-M085 $175 HF-0432-M085 $290 HF-0532-M085 $305 HF-1032-M085 $205

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgHF-0446-M085 $290 HF-0546-M085 $305 HF-1046-M085 $205

HAISIL 100 C18 3.2 and 4.6mm I.D. S.S.CartridgesHAISIL 100 C18 5µm

20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgHF-0232-M185 $175 HF-0432-M185 $290 HF-0532-M185 $305 HF-1032-M185 $205

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgHF-0446-M185 $290 HF-0546-M185 $305 HF-1046-M185 $205

HAISIL 300 3.2 and 4.6mm I.D. S.S.CartridgesHAISIL 300 C18 5µm

20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgHF-0232-W185 $190 HF-0432-W185 $330 HF-0532-W185 $340 HF-1032-W185 $225

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgHF-0446-W185 $330 HF-0546-W185 $340 HF-1046-W185 $225

HAISIL HL 3.2 and 4.6mm I.D. S.S.CartridgesHAISIL HL C18 5µm

20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgLF-0232-C185 $185 LF-0432-C185 $330 LF-0532-C185 $340 LF-1032-C185 $225

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgLF-0446-C185 $330 LF-0546-C185 $340 LF-1046-C185 $225

HEAVY 3.2 and 4.6mm I.D. S.S.CartridgesHEAVY C18 5µm

20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgVF-0232-C185 $190 VF-0432-C185 $330 VF-0532-C185 $340 VF-1032-C185 $225

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgVF-0446-C185 $330 VF-0546-C185 $340 VF-1046-C185 $225

55

HA ICART STA INLE S S STEE L CARTR IDGE COLUMNS

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HAICART CARTRIDGES (continued)STAINLESS STEEL CARTRIDGE COLUMNS

HA ICART STA INLE S S STEE L CARTR IDGE COLUMNS

PHALANX 3.2 and 4.6mm I.D. S.S.CartridgesPHALANX C18 5µm

20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgPF-0232-C185 $190 PF-0432-C185 $330 PF-0532-C185 $340 PF-1032-C185 $225

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgPF-0446-C185 $330 PF-0546-C185 $340 PF-1046-C185 $225

PROTO 200 3.2 and 4.6mm I.D. S.S.CartridgesPROTO 200 C4 5µm

20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgRF-0232-D045 $190 RF-0432-D045 $330 RF-0532-D045 $340 RF-1032-D045 $225

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgRF-0446-D045 $330 RF-0546-D045 $340 RF-1046-D045 $225

PROTO 200 C18 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgRF-0232-D185 $190 RF-0432-D185 $330 RF-0532-D185 $340 RF-1032-D185 $225

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgRF-0446-D185 $330 RF-0546-D185 $340 RF-1046-D185 $225

PROTO 300 3.2 and 4.6mm I.D. S.S.CartridgesPROTO 300 C4 5µm

20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgRF-0232-W045 $190 RF-0432-W045 $330 RF-0532-W045 $340 RF-1032-W045 $225

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgRF-0446-W045 $330 RF-0546-W045 $340 RF-1046-W045 $225

PROTO 300 C18 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgRF-0232-W185 $190 RF-0432-W185 $330 RF-0532-W185 $340 RF-1032-W185 $225

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgRF-0446-W185 $330 RF-0546-W185 $340 RF-1046-W185 $225

TARGA 3.2 and 4.6mm I.D. S.S.CartridgesTARGA C8 5µm

20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgTF-0232-C085 $175 TF-0432-C085 $330 TF-0532-C085 $340 TF-1032-C085 $225

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgTF-0446-C085 $330 TF-0546-C085 $340 TF-1046-C085 $225

TARGA C18 5µm20X3.2mm 5/pkg 40X3.2mm 2/pkg 50X3.2mm 2/pkg 100X3.2mm 1/pkgTF-0232-C185 $175 TF-0432-C185 $330 TF-0532-C185 $340 TF-1032-C185 $225

40X4.6mm 2/pkg 50X4.6mm 2/pkg 100X4.6mm 1/pkgTF-0446-C185 $330 TF-0546-C185 $340 TF-1046-C185 $225

200

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HA ICART STA INLE S S STEE L CARTR IDGE COLUMNS

See pages 66 - 67 for information about the Cartridge Holders required for these Cartridges

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HA I P E EK P E EK /T I TAN IUM CARTR IDGE COLUMNS

HAIPEEK CARTRIDGESPEEK/TITANIUM CARTRIDGE COLUMNS

PEEK and Titanium CartridgesThe HAIPEEK family of narrowboreHPLC cartridge columns is a unique highperformance product for LC-MS andother high-speed, small volume applica-tions. The 40 and 50mm sizes of 2.1mmI.D HAIPEEK HPLC Cartridges areindividually tested. A Certificate ofAnalysis, which includes the originalchromatogram and comprehensivetesting and sorbent documentation,accompanies each column. The 20mm x2.1mm cartridges excel as narrowboreguard columns, yet even though they arenot individually tested, the majority areroutinely used as very economical andefficient columns for high speed HPLCscreening and LC-MS applications. Seepages 66 - 67 for information about thecartridge holders required for HigginsAnalytical’s HAIPEEK Cartridge Columns.HAIPEEK cartridges are also compatiblewith PE/Brownlee MPLC cartridgeholders.

Guide to HAIPEEK Analytical Cartridge Part NumbersxK-0421-xxxx 40 x 2.1mm PEEK/titanium cartridgexK-0521-xxxx 50 x 2.1mm PEEK/titanium cartridgeSee Page 23 for complete Part Number information

CLIPEUS 2.1mm I.D. PEEK/Titanium CartridgesCLIPEUS C8 5µm

20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgCK-0221-C085 $180 CK-0421-C085 $270 CK-0521-C085 $275

CLIPEUS C18 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgCK-0221-C185 $180 CK-0421-C185 $270 CK-0521-C185 $275

CLIPEUS Cyano 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgCK-0221-CNP5 $190 CK-0421-CNP5 $285 CK-0521-CNP5 $290

CLIPEUS Phenyl 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgCK-0221-PHN5 $190 CK-0421-PHN5 $285 CK-0521-PHN5 $290

CLIPEUS Silica 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgCK-0221-SIL5 $180 CK-0421-SIL5 $270 CK-0521-SIL5 $275

HAISIL 100 2.1mm I.D. PEEK/Titanium CartridgesHAISIL 100 C8 5µm

20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgHK-0221-M085 $180 HK-0421-M085 $270 HK-0521-M085 $275

HAISIL 100 C18 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgHK-0221-M185 $180 HK-0421-M185 $270 HK-0521-M185 $275

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HA I P E EK P E EK /T I TAN IUM CARTR IDGE COLUMNS

HAISIL 300 2.1mm I.D. PEEK/Titanium CartridgesHAISIL 300 C18 5µm

20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgHK-0221-W185 $190 HK-0421-W185 $310 HK-0521-W185 $315

HAISIL HL 2.1mm I.D. PEEK/Titanium CartridgesHAISIL HL C18 5µm

20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgLK-0221-C185 $190 LK-0421-C185 $300 LK-0521-C185 $310

HEAVY 2.1mm I.D. PEEK/Titanium CartridgesHEAVY C18 5µm

20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgVK-0221-C185 $190 VK-0421-C185 $310 VK-0521-C185 $315

PHALANX 2.1mm I.D. PEEK/Titanium CartridgesPHALANX C18 5µm

20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgPK-0221-C185 $190 PK-0421-C185 $310 PK-0521-C185 $315

PROTO 200 2.1mm I.D. PEEK/Titanium CartridgesPROTO 200 C4 5µm

20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgRK-0221-D045 $190 RK-0421-D045 $310 RK-0521-D045 $315

PROTO 200 C18 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgRK-0221-D185 $190 RK-0421-D185 $310 RK-0521-D185 $315

PROTO 300 2.1mm I.D. PEEK/Titanium CartridgesPROTO 300 C4 5µm

20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgRK-0221-W045 $190 RK-0421-W045 $310 RK-0521-W045 $315

PROTO 300 C18 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgRK-0221-W185 $190 RK-0421-W185 $310 RK-0521-W185 $315

TARGA 2.1mm I.D. PEEK/Titanium CartridgesTARGA C8 5µm

20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgTK-0221-C085 $180 TK-0421-C085 $270 TK-0521-C085 $280

TARGA C18 5µm20X2.1mm 5/pkg 40X2.1mm 2/pkg 50X2.1mm 2/pkgTK-0221-C185 $180 TK-0421-C185 $270 TK-0521-C185 $280

See pages 66 - 67 for information about the Cartridge Holders required for these Cartridges

200

Page 62: HAI Price List 2005 - HPLC Column Manufacturer

CLIPEUS 10mm I.D. Semi-Prep Cartridges CLIPEUS C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges

30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgCF-0310-C181 $275 CF-0510-C181 $215 CF-1010-C181 $270

CLIPEUS C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgCF-0510-C185 $315 CF-1010-C185 $395

HAISIL 100 10mm I.D. Semi-Prep Cartridges HAISIL 300 C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges

30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgHF-0310-W181 $275 HF-0510-W181 $215 HF-1010-W181 $270

HAISIL 300 10mm I.D. Semi-Prep Cartridges HAISIL 300 C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges

50x10mm 1/pkg 100x10mm 1/pkgHF-0510-W185 $315 HF-1010-W185 $395

PROTO 200 10mm I.D. Semi-Prep Cartridges PROTO 200 C4 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges

30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgRF-0310-D041 $275 RF-0510-D041 $215 RF-1010-D041 $270

PROTO 200 C4 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgRF-0510-D045 $315 RF-1010-D045 $395

PROTO 200 C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges

30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgRF-0310-D181 $275 RF-0510-D181 $215 RF-1010-D181 $270

PROTO 200 C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgRF-0510-D185 $315 RF-1010-D185 $395

60

HAISpeed10 - A high performance10mm I.D. Cartridge SystemHigh throughput requirements forcombinatorial chemistry applicationsoften call for 10mm I.D. columns. HigginsAnalytical has developed a convenientcartridge system that offers an econom-ical solution for demanding combinatorialand semi-preprative needs. Even thougha HAISpeed10 cartridge costs a fractionof conventional 10mm HPLC column ofthe same length, they have the sameconsistently high performance of otherHiggins Analytical columns.

Each 5cm and 10cm cartridge column istested and is shipped with a CofA.

HAISpeed10 CARTRIDGES10mm I.D. Semi-Prep Cartridge Columns

Guide to HAISpeed 10 Cartridge Part NumbersxF-0310-xxxx 30 x 10mm cartridgexF-0510-xxxx 50 x 10mm cartridgexF-1010-xxxx 100 x 10mm cartridgeSee Page 23 for complete Part Number information

HA I S P E ED10 CARTR IDGE COLUMNS AND GUARDS

200

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HA I S P E ED10 CARTR IDGE COLUMN ORDER ING INFORMAT ION

PROTO 300 10mm I.D. Semi-Prep Cartridges PROTO 300 C4 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges

30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgRF-0310-W041 $275 RF-0510-W041 $215 RF-1010-W041 $270

PROTO 300 C4 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgRF-0510-W045 $315 RF-1010-W045 $395

PROTO 300 C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges

30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgRF-0310-W181 $275 RF-0510-W181 $215 RF-1010-W181 $270

PROTO 300 C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgRF-0510-W185 $315 RF-1010-W185 $395

PHALANX 10mm I.D. Semi-Prep Cartridges PHALANX C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges

30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgPF-0310-C181 $275 PF-0510-C181 $215 PF-1010-C181 $270

PHALANX C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgPF-0510-C185 $315 PF-1010-C185 $395

TARGA 10mm I.D. Semi-Prep Cartridges TARGA C18 10µm 10mm I.D. s.s. Guard Cartridges 10mm I.D. s.s. Semi-Prep Cartridges

30x10mm 2/pkg 50x10mm 1/pkg 100x10mm 1/pkgTF-0310-C181 $275 TF-0510-C181 $215 TF-1010-C181 $270

TARGA C18 5µm 10mm I.D. s.s. Semi-Prep Cartridges50x10mm 1/pkg 100x10mm 1/pkgTF-0510-C185 $315 TF-1010-C185 $395

See pages 66 - 67 for information about Cartridge Holders required for these Cartridges

NEW

10mm guard Cartridges

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Analytical Guard CartridgesThe complete range of sorbentsmanufactured by Higgins Analytical isavailable in 2cm x 2.1 or 3.2mm I.D.guard cartridges. Not only does thisprovide a wide selection of products forprotecting analytical and narrowboreHPLC columns, it also facilitates a widevariety of trace enrichment, columnswitching, and ballistic gradient applica-tions.

These 2cm long cartridges require areusable holder and are compatible withHiggins Analytical’s HAIPEEK GuardHolders as well as PE/Brownlee’sNewGuard Holder.

HAIPEEK & HAICART GuardsPEEK/TITANIUM and s.s. Guard Cartridges

Guide to HAIPEEK and HAICART Guard Cartridge Part NumbersxK-0221-xxxx 20 x 2.1mm PEEK/titanium guard cartridgexF-0232-xxxx 20 x 3.2mm stainless steel guard cartridgeSee Page 23 for complete Part Number informationNote: Old HAICART P/Ns such as H118, T118, C1PH, etc. can still be used when ordering.

GUARD CARTR IDGES

CLIPEUS Guard CartridgesCLIPEUS C8 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgCK-0221-C085 $180 CF-0232-C085 $180

CLIPEUS C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgCK-0221-C185 $180 CF-0232-C185 $180

CLIPEUS Cyano 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgCK-0221-CNP5 $190 CF-0232-CNP5 $185

CLIPEUS Phenyl 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgCK-0221-PHN5 $190 CF-0232-PHN5 $185

CLIPEUS Silica 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgCK-0221-SIL5 $180 CF-0232-SIL5 $180

HAISIL 100 Guard CartridgesHAISIL 100 C8 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgHK-0221-M085 $180 HF-0232-M085 $180

HAISIL 100 C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgHK-0221-M185 $180 HF-0232-M185 $180

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GUARD CARTR IDGE ORDER ING INFORMAT ION

HAISIL 300 Guard CartridgesHAISIL 300 C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgHK-0221-W185 $190 HF-0232-W185 $190

HAISIL HL Guard CartridgesHAISIL HL C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgLK-0221-C185 $190 LF-0232-C185 $190

HEAVY Guard CartridgesHEAVY C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgVK-0221-C185 $190 VF-0232-C185 $190

PHALANX Guard CartridgesPHALANX C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgPK-0221-C185 $190 PF-0232-C185 $190

PROTO 200 Guard CartridgesPROTO 200 C4 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgRK-0221-D045 $190 RF-0232-D045 $190

PROTO 200 C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgRK-0221-D185 $190 RF-0232-D185 $190

PROTO 300 Guard CartridgesPROTO 300 C4 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgRK-0221-W045 $190 RF-0232-W045 $190

PROTO 300 C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 0X3.2mm 5/pkgRK-0221-W185 $190 RF-0232-W185 $190

TARGA Guard CartridgesTARGA C8 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgTK-0221-C085 $180 TF-0232-C085 $180

TARGA C18 5µm2.1mm I.D. Peek/Titanium 3.2mm I.D. Stainless Steel

20X2.1mm 5/pkg 20X3.2mm 5/pkgTK-0221-C185 $180 TF-0232-C185 $180

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HA I S P E ED 2 0 - PREP GUARD CARTR IDGES

HAISpeed20 guard cartridges pro-vide unique Protection for 20mmI.D. prep columns.“Prep is a dirty business,” and expensivecolumns can be quickly destroyed bycrude samples if proper precautions arenot taken. Higgins Analytical’sHAISpeed20 Prep Guard Systemprovides effective column protectionwithout compromising performance. Inaddition to an efficiently packed bedwhich retains strongly absorbing junkthat would change the selectivity of theprep column, these unique guardcartridges have 0.5µm frits that removeparticulate contaminants as well.

HAISpeed20 CARTRIDGES20mm I.D. Prep Guard Cartridge Columns

Guide to HAISpeed 20 Cartridge Part NumbersxF-0220-xxxx 25 x 20mm cartridgeSee Page 23 for complete Part Number information

Typical 250x20mm HAISIL 100 C18 5µmTest Chromatogram.

The same 250x20mm 5µm column shown in the top chromatogram retested with a HAISPEED 20 Guard Cartridge in line.

HAISpeed20 guard cartridges provideconvenience and performance

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HA I S P E ED 2 0 ORDER ING INFORMAT ION

HAISIL 100 20mm I.D. Prep Guard Cartridges HAISIL 100 C18 10µm 20mm I.D. s.s. Prep Guard Cartridges

25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkHF-0220-M181 $295 HF-0320-M181 $150 HF-0520-M181 $200

CLIPEUS 20mm I.D. Prep Guard Cartridges CLIPEUS C18 10µm 20mm I.D. s.s. Prep Guard Cartridges

25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkCF-0220-C181 $295 CF-0320-C181 $150 CF-0520-C181 $200

HAISIL 300 20mm I.D. Prep Guard Cartridges HAISIL 300 C18 10µm 20mm I.D. s.s. Prep Guard Cartridges

25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkHF-0220-W181 $295 HF-0320-W181 $150 HF-0520-W181 $200

PROTO 200 20mm I.D. Prep Guard Cartridges PROTO 200 C4 10µm 20mm I.D. s.s. Prep Guard Cartridges

25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkRF-0220-D041 $295 RF-0320-D041 $150 RF-0520-D041 $200

PROTO 200 C18 10µm 20mm I.D. s.s. Prep Guard Cartridges25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkRF-0220-D181 $295 RF-0320-D181 $150 RF-0520-D181 $200

PROTO 300 20mm I.D. Prep Guard Cartridges PROTO 300 C4 10µm 20mm I.D. s.s. Prep Guard Cartridges

25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkRF-0220-W041 $295 RF-0320-W041 $150 RF-0520-W041 $200

PROTO 300 C18 10µm 20mm I.D. s.s. Prep Guard Cartridges25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkRF-0220-W181 $295 RF-0320-W181 $150 RF-0520-W181 $200

PHALANX 20mm I.D. Prep Guard Cartridges PHALANX C18 10µm 20mm I.D. s.s. Prep Guard Cartridges

25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkPF-0220-C181 $295 PF-0320-C181 $150 PF-0520-C181 $200

TARGA 20mm I.D. Prep Guard Cartridges TARGA C18 10µm 20mm I.D. s.s. Prep Guard Cartridges

25x20mm 2/pkg 35x20mm 1/pk 50x20mm 1/pkTF-0220-C181 $295 TF-0320-C181 $150 TF-0520-C181 $200

Both a Filter and a Guard

The 0.5µm pore size frits onHAISpeed20 cartridges provide uniquebuilt-in filtration for maximum columnprotection. These cartridges are shippedwith end caps that prevent solvent lossand protect the cartridge and frits.

200

NEW

35x20mm and

50x20mm

Even greater capacity

for trapp

ing and

purification

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66

No Tools Required for Leak-FreeOperationEven though Higgins Analytical cartridgeholders do not require any tools forassembly and disassembly, their leak-freeoperation assures that maximum per-formance will be achieved in yourdemanding, high-speed separations.

The Holder’s light weight also makes iteasy to handle and assures its compati-bility in challenging plumbing situations.

Cartridge HoldersHolders for 2.1, 3.2, 4.6,10, and 20mm I.D. Cartridges

Guide to Cartridge Holder Part NumbersHK-GUARD-FF Female/Female Holder for 2cm cartridgeHK-GUARD-FM Female/Male Direct Connect Holder for 2cm cartridgeHK-HOLD-40 Holder for 4cm x 2.1, 3.2, 4.6mm cartridgeHK-HOLD-50 Holder for 5cm x 2.1, 3.2, 4.6mm cartridgeHK-HOLD-100 Holder for 10cm x 2.1, 3.2, 4.6mm cartridge10-HOLD-30 Holder for 30x10mm cartridge10-HOLD-50 Holder for 50x10mm cartridge10-HOLD-100 Holder for 100x10mm cartridge20-HOLD-25 Holder for 20 x 25mm cartridge

CARTR IDGE HOLDERS

Higgins Analytical Cartridges and Holders enable a wide range of applications and techniques

Direct-Connect Guards

Loop-Column applications

Page 69: HAI Price List 2005 - HPLC Column Manufacturer

CARTR IDGE HOLDER ORDER ING INFORMAT ION

67

2cm Guard Cartridge HoldersDirect Connect Holders

Female/Male Standard Female/Male for Wateers ColumnHK-GUARD-FM $160 WK-GUARD-FM $160

Symmetrical Connection HoldersFemale/Female HolderHK-GUARD-FF $175

Holders for Analytical Cartridge ColumnsAnalytical Cartridge Holders

2cm Cartridges 4cm Cartridges 5cm Cartridges 10cm CartridgesHK-HOLD-20 $175 HK-HOLD-40 $175 HK-HOLD-50 $180 HK-HOLD-100 $185

Note: HK-HOLD-20 is the same as HK-GUARD-FF

Holders for 10mm I.D. Semi-Prep Guards and CartridgesHAISPEED 10 Cartridge Holders

3cm Cartridges 5cm Cartridges 10cm Cartridges10-HOLD-30 $175 10-HOLD-50 $180 10-HOLD-100 $185

Holders for 20mm I.D. Prep Guard CartridgesHAISPEED 20 Guard Cartridge Holders

20x25mm Cartridges 20x35mm Cartridges 20x50mm Cartridges20-HOLD-25 $270 20-HOLD-35 $270 20-HOLD-50 $270

Higgins Analytical manufacturers the widest range of guard cartridges in the world

Analytical (2.1 and 3.2mm)

Semi-Prep (10mm)

Prep Scale (20mm)

NEW

20x35 & 20x50mm Holders

See page 63 for the New Cartriges

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HA I F I LTER - H IGH P ERFORMANCE IN - L INE F I LTER - NO TOOLS REQU IRED

A simple, yet effective, means ofprotection is to place a filter in theflow path between the sampleinjection valve and the HPLC col umn.While this technique is commonlyused, it is also com monly plagued byleaky holders and significantperformance loss. TheHiggins Analytical HAIFILTER resolvesall of these problems!

Simplicity and ConvenienceThe Higgins Analytical HAIFILTER pro videsefficient and leak-free in-linefiltration, yet it does notrequire any tools. To make iteven more convenient, theHAIFILTER is designed toconnect directly to the inletfitting on the HPLC column,maximizing per- formance, and minimizinghardware. The 0.5µm porosity frits willremove much of the particulate contami -nation that is largely responsible for col umnpressure build up and plugging. The holder’sinert construction, and the avail ability ofPEEK and titanium as well as stainless steelfrits, insures that the HAIFILTER can addressall of your in-line filtration needs.

Two Convenient FormatsHAIFILTER is now available in two for mats.The leak-free, finger-tight, and effi cientperformance are qualities of the HAIFILTERthat have made it a popular product,replacing the competition’s awk ward andleaky alternatives in hundreds oflaboratories worldwide. The direct -connect feature in the original designmakes the HAIFILTER FM ideal for pro -tecting HPLC columns while the symmet -rical female thread fittings on each end ofHAIFILTER FF provide unequaled flexibil ityto address your HPLC system’s filtra tionrequirements.

KEY FEATURES:

• Low cost, yet effective column protection

• Direct column connection and symmetrical versions

• No tools required • Leak free operation

• 0.5µm filters in stainless steel, titanium or PEEK

• Inert construction’s threads won’t oxidize or gall

PerformanceTry the experiment summarized by thechromatograms above with another man -ufacturer’s in-line filter. You may be verysurprised how much more other in linefilters contribute to peak broadeningwhen comparison to the miniscule 28µL2

at 200µL/min volume-variance that is

HPLC Columns without a HAIFILTER

HPLC Columns with a HAIFILTER

N=24,664

N=19,525

N=24,336N=17,708

250x4.6mm (P/N LS-2546-C185)

250x4.6mm (P/N LS-2546-C185)

250x2.1mm (P/N HS-2546-M185)

250x2.1mm (P/N HS-2546-M185)

HAIFILTER “We found the HAIFILTER to be an ideal solution for our high performance

in-line filtration needs, and it doesn’t require any tools or wrenches!”

obtained with the HAIFILTER. Thisexcep tional efficiency is confirmed bychroma tographers reporting that using aHAIFILTER permits the routine analysis ofsolid-phase sample extracts by LC/MSwith good efficiency and greatlyenhanced HPLC column life.

REINTRODUCED!

Even tho

ugh HAIF

ILTER wa

s

removed

from our

catalog s

everal

years ago

, it conti

nues to b

e so

popular w

ith our o

ld custom

ers,

we’ve de

cided to

include

it again.

Best par

t?

Price is u

nchanged

!

Page 71: HAI Price List 2005 - HPLC Column Manufacturer

HA I F I LTER & PARTS AND ACCES SOR I E S ORDER ING INFORMAT ION

69

PARTS AND ACCES SOR I E S

Micro Column Connector KitKit facilitates plumbing a Piccolo, Sprite, Capellini 6-32, or a Caπllary to your instrument

MIC-KIT $100Kit Contains:

6-32 PEEK Bushings (4 each)10-32 PEEK Long Bushings (2 each)Green Sleeve 0.155 x 0.025” (4 each)Brown Sleeve 0.062 x 0.015” (2 each)Fused Silica Capillaries 10cm x 150µm (4 each)

HAIFILTER FM (Female/Male) Starter KitsHF-FKIT HAIFILTER FM (Standard) $270HF-WKIT HAIFILTER FM (for Waters HPLC columns) $270HF-RKIT HAIFILTER FM (for Rheodyne injection valves - $270

Specify valve model when ordering)

Note: HAIFILTER FM Starter Kits include a package of 10 filters (HF-SFILT), a 10cm piece ofPEEK tubing, and a Delrin bushing and a PEEK ferrule.

HAIFILTER FF (Female/Female) Starter KitFF-FKIT HAIFILTER FF $270

Note: The HAIFILTER FF Starter Kit includes a package of 10 filters (HF-SFILT), two 10cmpieces of PEEK tubing, and two Delrin bushings and two PEEK ferrules.

Replacement FiltersAll replacement kits contain 10 filters per packageHF-SFILT Stainless steel HAIFILTER replacement filters pkg of 10 $85HF-TFILT Titanium HAIFILTER replacements filters pkg of 10 $140HF-PFILT PEEK HAIFILTER replacements filters pkg of 10 $160

HAIFILTER Holders and Replacement Filters

FlexibilityNot only is the HAIFILTER available intwo formats, it can also be directlyconnected to a sample injection orswitching valve. The photograph to theright demon strates how the HAIFILTERhas been designed to clear the bushingson a popular Rheodyne sample injectionvalve.

FILTER replacement is easy and requires no tools

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TECHN ICAL SUPPORT AND ORDER ING INFORMAT ION

Expert and Responsive TechnicalSupport As a Higgins Analytical Customer seekingtechnical support or product information,you will eliminate long waits andexpensive phone calls, and you will neverbe transferred from one person toanother only to end up in someone’sphone mail! By simply faxing (1-650-988-8931) or e-mailing ([email protected])your question to our Technical SupportGroup, you will receive a prompt writtenresponse. If we don’t know the answer,we will tell you so – but, that doesn’thappen very often. Additional up-to-dateproduct and application information canbe found by pointing your browser at ourweb site. www.higanalyt.com

Easy Ordering and Rapid Delivery Our worldwide network of qualifieddistributors can provide expert supportand prompt delivery from their localinventory. Our catalog-listed items aregenerally shipped from the factory withina day of receiving the order. In the eventthat we have not provided you with anear by distributor, we can ship an orderto you directly from the factory by avariety of carriers.

In order to further simplify the process foryou and your purchasing department,

Higgins Analytical and many of its distribu-tors accept credit card orders. Paymentterms for all other orders are net 30 daysfrom date of invoice.

Product Availability Higgins Analytical products are availableworldwide through a network of respon-sive distributors. These organizations canprovide qualified technical support andprompt product delivery. The names canbe found by sending us a fax (650-988-8931) or by checking our web site which,in addition to names, addresses and phonenumbers, contains many hyperlinked e-mail and URL addresses.

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WARRANTY, PATENTS AND TRADEMARKS

HIGGINS ANALYTICAL PRODUCTSMAY BE PURCHASED AND ARE SOLDONLY ON THE FOLLOWING WARRAN-TY TERMS AND CONDITIONS. ADDI-TIONAL OR DIFFERENT TERMS,WHETHER IN AN ORDER, ACKNOWL-EDGMENT, INVOICE OR OTHER DOCU-MENT, SHALL NOT BECOME PART OFOR VARY THE FOLLOWING TERMSUNLESS AGREED TO IN WRITING BYHIGGINS ANALYTICAL.

LIMITED WARRANTY

Higgins Analytical products are manufac-tured and quality controlled to the highestindustry standards, and Higgins Analyticalwarrants that the products, when properlyapplied in accordance with HigginsAnalytical's standards therefor, will performfor a period of thirty (30) days after deliverysubstantially as described in HigginsAnalytical's then current specifications forthe product. If a customer believes a prod-uct does not comply with such warranty, thecustomer shall, within the warranty period,report the purported defect to HigginsAnalytical and cooperate fully with HigginsAnalytical in determining the conditions andcause of any purported defect and, thereby,obtain a returned goods authorization fromHiggins Analytical or the place of purchase.If Higgins Analytical determines the allegeddefect occurred as a result of misuse, neg-lect, improper installation, repair, alteration,application or accident, rapid depressuriza-tion, removal of column end fittings, unusualphysical or electrical stress or improper han-dling procedure, the warranty shall be voidand ineffective.

EXPRESS LIMITATIONS

HIGGINS ANALYTICAL PRODUCTS AREMANUFACTURED FOR USE INRESEARCH IN CHEMICAL MANUFAC-TURING LABORATORIES ONLY ANDARE NOT INTENDED FOR USE INCLINICAL OR DIAGNOSTIC APPLICA-TIONS. THE HAISPEED10 AND HAI-SPEED20 CARTRIDGE HOLDERS ARERATED FOR USE UP TO 3,000 PSI(200BAR) WITH FLUIDS THAT ARE LIQ-UID AT STP (STANDARD TEMPERATUREAND PRESSURE) ONLY. SERIOUS BODI-LY INJURY OR DEATH MAY RESULT IF AHAISPEED HOLDER BROKE WHILEWORKING WITH SUPERCRITICAL FLU-IDS.

DISCLAIMER

EXCEPT AS EXPRESSLY PROVIDED, ALLWARRANTIES, CONDITIONS, REPRE-SENTATIONS, INDEMNITIES ANDGUARANTEES, WHETHER EXPRESS ORIMPLIED, ARISING BY LAW, CUSTOM,PRIOR ORAL OR WRITTEN STATE-MENTS BY HIGGINS ANALYTICAL OROTHERWISE (INCLUDING, BUT NOTLIMITED TO, ANY WARRANTY FORMERCHANTABILITY OR FITNESS FOR APARTICULAR PURPOSE) ARE OVERRID-DEN, EXCLUDED AND DISCLAIMED.

EXCLUSIVE REMEDY

In the event a customer establishes abreach of the above warranty, customer'sexclusive remedy against Higgins Analytical,and Higgins Analytical's sole liability, is, at itsoption, the replacement of the defectiveproduct or the refund of the purchase pricetherefor.

NO CONSEQUENTIAL DAMAGES

HIGGINS ANALYTICAL SHALL NOT BERESPONSIBLE FOR ANY CONSEQUEN-TIAL, INDIRECT, SPECIAL, PUNITIVE ORINCIDENTAL DAMAGES, WHETHERFORESEEABLE OR UNFORESEEABLE,INCLUDING, BUT NOT LIMITED TO,CLAIMS FOR LOSS OF GOODWILL,PROFITS, USE OF MONEY OR USE OFPRODUCTS, STOPPAGE OF OTHERWORK OR IMPAIRMENT OF OTHERASSETS, ARISING OUT OF BREACH OFEXPRESS OR IMPLIED WARRANTY,BREACH OF CONTRACT, MISREPRESEN-TATION, NEGLIGENCE, STRICT LIABILITYIN TORT OR OTHERWISE, EXCEPTONLY IN THE CASE OF PERSONALINJURY WHERE AND TO THE EXTENTTHAT APPLICABLE LAW REQUIRESSUCH LIABILITY.

PATENTS AND TRADEMARKS

Kromasil is a trademark of Eka Nobel.NewGuard and MPLC are trademarks ofPerkin-Elmer. PICOTAG is a trademark ofWaters. CAPELLINI, CLIPEUS, HAISIL,HAICART, HAIFILTER, HAIPEEK, HAISPEED, HEAVY, TARGA®, PHALANX,PROTO, HAIGUARD, PICCOLO, SPRITE,and Caπllary are trademarks of HigginsAnalytical, Inc.

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Higgins Analytical, Inc.1235 Pear Avenue, Suite 105Mountain View, California, 94043 USAPhone: 650-988-8930 or 800-988-6889Fax: [email protected] www.higanalyt.com

Expert Manufacturers of HPLC Consumables

Higgins Analytical develops and manufactures a wide range of stainlesssteel and PEEK (polyetheretherketone)High Performance Liquid Chromatography(HPLC) columns and cartridges. Theseunique offerings address many needs ofchromatographers desiring fast analysistimes, efficient system and columnprotection, and chemical inertness.

Special application areas include LC/MS,combinatorial chemistry, column switchingtechniques, in-line filtration, and guardcolumn requirements. Products aresupplied and supported worldwide by a network of responsive distributors.

We are a quality-oriented chromatographycompany dedicated to providingperformance products, expert support,and rapid delivery to research and quality control laboratories worldwide.

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HigginsAnalytical

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