Reporter Gene Imaging

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Reporter Gene Imaging

description

Reporter Gene Imaging. Using antibody fragments as artificial “receptors”. Hapten 1 -X*. Hapten 2 -Y*. Ligands. Hapten-X*. Antibody fragments. B. A. Membrane anchoring domains. mRNA. mRNA. mRNA. Constitutive promoter. tissue specific promoter. DNA. Vector. - PowerPoint PPT Presentation

Transcript of Reporter Gene Imaging

Page 1: Reporter Gene Imaging

Reporter Gene Imaging

Page 2: Reporter Gene Imaging

Using antibody fragments as artificial “receptors”

Ligands

Antibody fragments

Membraneanchoringdomains

Hapte

n-X*

Hapte

n 1-X

*

Hapte

n 2-Y

*

AB

Vector

mRNA

DNA

mRNA mRNA

tissuespecificpromoter

Constitutivepromoter

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Antibody reporter DAbR1

U87-DAbR1

0

10

20

30

40

50

60

HSVtk/FHBG

Reporter Gene / Reporter Probe

%ID

/g

tumor+ tumor-

blood

DAbR1/AABD

Liver

PET/CT with Y86-DOTA

Comparison with HSVtk reporter

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Translational Molecular Imaging

Hohe Sensitifität (pmol/l)Nachweis von RezeptorenMöglich

Minimalste Stoffmengen!Keine pharmakologischenEffekte

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Untersuchung der myokardialen Perfusion und des Sauerstoffverbrauchs mit 11C-1-acetate

Ng et al. Am J Physiol 1994

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Sun et al. J Nucl Med 1998

Untersuchung der myokardialen Perfusion und des Sauerstoffverbrauchs mit 11C-1-acetate

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Bengel et al.EJNM 2000

Untersuchung der myokardialen Effizienz mit 11C-1-acetate

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Multimodale PET/CT Bildgebung

LAD Stenose ohne Ischämie

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Multimodale PET/CT Bildgebung

LCX Stenose mit ausgeprägter Ischämie