SoGAT XVI, Langen 3 rd July 2003

Ro / BSD HessenInstitut für Transfusionsmedizin

SoGAT XVI, Langen 3rd July 2003

Immediate Parvo B19 NAT testing of pooled donor samples: logistics and problems

W. Kurt RothRed Cross Blood Transfusion Service Baden-Württemberg – Hessen,

Institute Frankfurt am Main


B19 / HAV-PCR: Testing Strategy

B19 and HAV PCR in parallel to HCV, HIV and HBV PCR Aliquots of same extract as for HCV, HIV, HBV Relevant for all blood components incl. platelets Quantitative TaqMan PCR with internal control sequence 95% detection limit: 103 IU / ml (B19 and HAV) B19 positive Pools 105 IU / ml:

– Positive donation identified

– Positive donation destroyed B19 positive Pools < 105IU / ml:

– Positive donation not identified

– All donations released (weak positive)


Pool Deconstruction HCV, HBV, HIV, HAV, B19

PCR-only positives PCR and antibody positives

12 x 8

8 x

12

1 x 94

individual testing


HAV / Parvo B19-PCR: Results

Positives / Tested Positives / Tested

Virus Pools n / n (%) Donations n / n Rate

HAV 2 / 22 368 (0.009) 2 / 1 832 802 1 : 0.9 Mill

B19 121 / 22 368 (0.54) 162 / 1 832 802 1 : 11 000

April 2000 – May 2003: Sensitivity 105 IU / ml

Rate of B19 positives at a sensitivity of 103 IU / ml: 1 : 700


0

2

4

6

8

10

12

14

Parvo B19 Positives per MonthP

ositi

ves

105

(n)

Total

Frankf

West


Indications for B19-negative components

Parvo B19-negative (< 103 IU / ml) components upon specific written request; individual label: „Parvovirus B19 not detectable“– Patients with BMT, SCT

– B19 antibody-negative pregant women in the 1.and 2. trimenon

– HIV patients

– Additional risk groups e.g.• transient aplastic crisis • pure red cell aplasia

Parvo B19-negative and weak positive (> 103 ; < 105 IU / ml) components for all patients at no specific risk (no label); standard quality


Parvo-B19 Seroconversion

Vorspende Spende Folgespende 2. Folgesp.Datum IgM/

GDatum Konz

ExpIgM IgG Datum Hs-

PCRIgM IgG Wb

NS1IgM Wb

NS104.07.00 - 17.01.01 12 - - 02.08.01 - + ++ +28.03.00 - 14.07.00 12 - - 15.02.01 - ? ++ ++28.02.00 - 20.06.00 11 - - 27.10.00 + ? ++ ? - +15.02.99 15.05.00 11 + - 06.08.00 + + ++ + - ++12.01.00 - 12.04.00 11 - - 05.07.00 + + ++ ? - ?15.01.01 - 16.03.01 8 + - 22.06.01 + - ++ +09.02.00 - 03.05.00 8 - - 09.08.00 + + ++ +30.11.00 - 30.03.01 8 + - 29.06.01 + + ++ +25.08.00 - 27.10.00 7 - - 26.01.01 + + ++ - ? -09.02.00 - 03.05.00 6 + ? 23.08.00 + + ++ ++ + +++19.01.00 - 10.05.00 5 ++ + 17.01.01 - + ++ +++02.04.01 - 02.07.01 5 ++ + 11.09.01 + + ++ +++

Medac IgM and IgG ELISA; Mikrogen IgG/IgM recomBlot

SoGAT XVI, Langen 3 rd July 2003

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