+ rIgG1

13B8.2

100 101 102 100 101 102

Anti-ceramide antibody

Eve

nts

0

40

Non-permeabilizedJurkat T cells

PermeabilizedJurkat T cells

10 min-treatment 4 h-treatment

+ rIgG1

13B8.2

+ rIgG1

13B8.2+ rIgG1

13B8.273%

21%

50%

11%

Eve

nts

A40

0100 101 102100 101 102

B

0

10

20

30

40

50

Cer

amid

e-po

siti

veJu

rkat

T c

ells

(%

)

Inhibitor - - FB1 FB1 Imi Imi Myr Myr- +rIgG1 13B8.2 +- - + - +

Fig.3