S o kra t e sG ro u p , F o rch st ra sse 6 0 , 8 0 0 8 Z ...
Antidia b Acta Pharmakon Issue 1, V ol. (1 ), M a rch 2 0...
6
, n o k a m r a h P a t c A 1 ( . l o V , 1 e u s s I , ) y r a u n a J - 5 1 0 2 h c r a M [7 3 ] f o t c a r t x E c i l o n a h t e M f o y t i v i t c a c i t e b a i d i t n A f o n o i t a u l a v E a c i n h c e t o r y p a i n e d a t p e L d e c u d n i n a x o l l A n i e n c e D ) . k s r o F ( e c i M n i s e t e b a i D r a m u K l i n u S *1 i l u h c r a K . S . M , 2 r a m u K n i h c a S , 1 1 r e e v a h a M r e k n a h t r e e T , y c a m r a h P f o e g e l l o C r e e v a h a M r e k n a h t r e e T d a b a d a r o M , y t i s r e v i n U – 1 0 0 4 4 2 . a i d n I , h s e d a r P r a t t U , 2 l a p o h B , ) I R B P ( e t u t i t s n I h c r a e s e R l a c i d e m o i B e l c a n n i P . a i d n I , h s e d a r P a y h d a M , . 1 N O I T C U D O R T N I r e d r o s i d c i l o b a t e m c i n o r h c a s i s u t i l l e m s e t e b a i D d e z i r e t c a r a h c , y c n e i c i f e d n i l u s n i m o r f g n i t l u s e r f o m s i l o b a t e m d e r e t l a , a i m e c y l g r e p y h y b d e s a e r c n i n a d n a d i p i l , n i e t o r p , s e t a r d y h o b r a c r n o i t a c i l p m o c r a l u c s a v f o k s i . s ] 1 [ I t n e s e r p n u t i s W e h t y l b i s s o p s i s e t e b a i d n o i t a t s e g r a l s ’ d l r o n o k a m r a h P a t c A 1 e u s s I l o V , . 1 ( 7 3 , 5 1 0 2 h c r a M , ) -2 4 h c r a e s e R e l c i t r A t a e n i l n o e l b a l i a v A m o c . n o k a m r a h p a t c a . w w w O F N I E L C I T R A d e v i e c e R 2 0 b e F , 5 1 0 2 d e s i v e R 7 1 r a M 5 1 0 2 , d e t p e c c A 5 2 r a M 5 1 0 2 , : s d r o W y e K , n a x o l l A c i t e b a i d i t n A y t i v i t c a s r e t e m a r a p l a c i m e h c o i B a i n e d a t p e L a c i n h c e t o r y P e d i m a l c n e b i l G : r o h t u A g n i d n o p s e r r o C r a m u K l e e n u S : d i l i a m E . l i a m g @ 1 8 7 9 3 r a m u k l i n u s m o c T C A R T S B A e h t r o f d e t a u l a v e s a w y t i v i t c a c i t e b a i d i t n A e l o h w f o t c a r t x e c i l o n a h t e m f o t n a l p a c i n h c e t o r y P a i n e d a t p e L e h t f o t c a r t x e c i l o n a h t e M . e n c e D ) . k s r o f ( a c i n h c e t o r y P a i n e d a t p e L s a w t h g i e w y d o b g k / g m 0 0 2 f o e s o d a t a l a c i m e h c o i b e h T . e c i m c i t e b a i d d e c u d n i n a x o l l A o t d e r e t s i n i m d a G S , T O G S s a h c u s s r e t e m a r a p o s l a e r e w s e d i r e c y l g i r t d n a , l o r e t s e l o h c , T P e h t f o t c a r t x e c i l o n a h t e m e h T . s l a m i n a l a t n e m i r e p x e e h t n i d e s s e s s a a c i n h c e t o r y P a i n e d a t p e L y t i v i t c a c i m e c y l g r e p y h i t n a t n a c i f i n g i s d e t i b i h x e T P G S e k i l s r e t e m a r a p l a c i m e h c o i b f o s l e v e l e h t d e c u d e r o s l a t i d n a 0 5 ( d n a ) 2 2 . 7 ± 6 1 . 2 2 1 ( l o r e t s e l o h c , ) 2 7 . 7 ± 3 3 . 6 5 ( T O G S , ) 9 8 . 5 ± 5 . o t r a e n s a w t c a r t x e c i l o n a h t e m f o y c n e t o p e h T . ) 8 2 . 9 ± 3 3 . 7 0 1 ( s e d i r e c y l g i r t d e w o h s s n o i t a g i t s e v n i t n e s e r P e h T . e d i m a l c n e b i l g d r a d n a t s f o t c e f f e e h t f o t n a l p e l o h w t a h t a c i n h c e t o r y p a i n e d a t p e L o p s a h c i t e b a i d i t n a l a i t n e t . s t c e f f e
Transcript of Antidia b Acta Pharmakon Issue 1, V ol. (1 ), M a rch 2 0...
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 73 ]
fo tcartxE cilonahteM fo ytivitca citebaiditnA fo noitaulavE acinhcetoryp ainedatpeL decudni naxollA ni enceD ).ksroF(
eciM ni setebaiD
ramuK linuS *1 iluhcraK .S.M , 2 ramuK nihcaS , 1 1 reevahaM reknahtreeT ,ycamrahP fo egelloC reevahaM reknahtreeT dabadaroM ,ytisrevinU –
100442 .aidnI ,hsedarP rattU , 2 lapohB ,)IRBP( etutitsnI hcraeseR lacidemoiB elcanniP .aidnI ,hsedarP ayhdaM ,
.1 NOITCUDORTNI
redrosid cilobatem cinorhc a si sutillem setebaiD deziretcarahc ,ycneicifed nilusni morf gnitluser
fo msilobatem deretla ,aimecylgrepyh yb desaercni na dna dipil ,nietorp ,setardyhobrac
r noitacilpmoc ralucsav fo ksi .s ]1[ I tneserp nutis W eht ylbissop si setebaid noita tsegral s’dlro
nokamrahP atcA 1 eussI loV , . 1( 73 ,5102 hcraM ,) - 24
hcraeseR elcitrA
ta enilno elbaliavA moc.nokamrahpatca.www
OFNI ELCITRA
devieceR 20 beF , 5102 desiveR 71 raM 5102 ,
detpeccA 52 raM 5102 ,
:sdroW yeK ,naxollA citebaiditnA ytivitca sretemarap lacimehcoiB ainedatpeL
acinhcetoryP edimalcnebilG
:rohtuA gnidnopserroC
ramuK leenuS :di liamE
.liamg@18793ramuklinusmoc
TCARTSBA
eht rof detaulave saw ytivitca citebaiditnA elohw fo tcartxe cilonahtem fo tnalp acinhcetoryP ainedatpeL eht fo tcartxe cilonahteM .enceD ).ksrof(
acinhcetoryP ainedatpeL saw thgiew ydob gk/gm 002 fo esod a ta lacimehcoib ehT .ecim citebaid decudni naxollA ot deretsinimda
GS ,TOGS sa hcus sretemarap osla erew sedirecylgirt dna ,loretselohc ,TP eht fo tcartxe cilonahtem ehT .slamina latnemirepxe eht ni dessessa
acinhcetoryP ainedatpeL ytivitca cimecylgrepyhitna tnacifingis detibihxe TPGS ekil sretemarap lacimehcoib fo slevel eht decuder osla ti dna
05( dna )22.7±61.221( loretselohc ,)27.7±33.65( TOGS ,)98.5±5. ot raen saw tcartxe cilonahtem fo ycnetop ehT .)82.9±33.701( sedirecylgirt dewohs snoitagitsevni tneserP ehT .edimalcnebilg dradnats fo tceffe eht
fo tnalp elohw taht acinhcetoryp ainedatpeL op sah citebaiditna laitnet.stceffe
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 83 ]
htob eht tuohguorht redrosid cilobatem gniworgecneH .seirtnuoc gnipoleved dna depoleved , lla
ap desongaid ylwen neit epyt htiw st - setebaid II dna yrateid fo lairt laitini na evah dluohs stneitap ynam ,revewoH .snoitacifidom esicrexe na tuohtiw tnemtaert lacigolocamrahp eriuqer .ytivitca lacisyhp dna noitirtun fo lairt lanoitpo
yparehtonoM htiw mroftem ro aerulynoflus nieb nac .yparehtocamrahp enil tsrif sa desu
gnitsaf fi ,revewoH naht rehgih si esoculg doolbsi esoculg doolb modnar ro ld/gm 052 rehgih
naht laitini sa desu eb dluohs nilusni ,ld/gm 004hguohtlA .ypareht laro dna nilusni ,
cimecylgopyh eht era stnega jam eht ni sreyalp roert tub sutillem setebaid eht fo tnemta evah yeht
yltnacifingis ot liaf dna stceffe edis tnenimorpsetebaid fo esruoc eht retla . ]5 ,4 ,3 ,2[ dlrow ehT
taht dednemmocer sah noitazinagro htlaehbaid rof tnemtaert tnalp lanoitidart narraw sete t
noitaulave rehtruf . ]6[ larutan A itna - citebaid eht ni tceffe laicifeneb a trexe dluoc tnega noiterces nilusni gnicnahne yb noitautis citebaid
dna / pmi yb ro noitca nilusni gnikcimim/gnivor .[ ]7 woN a dna yratnemelpmoc fo esu eht ,syad
eht yllaicepse dna enicidem evitanretla gnisaercni neeb evah slacinatob fo noitpmusnoc
yldipar ediwdlrow eht fo esuaceb yltsomedis tneuqerf ssel yldesoppus - nehw stceffe
pmoc enicidem nredom ot dera . ]8[ cetoryP ainedatpeL acinh )ksrof( enceD
,)eaecadaipelcsA( pmihK sa nwonk ylnommoc a si ,cibarA ni hkerem ;natsikaP dna aidnI ni
tnalp lainnerep ]9[ t t ni yltnadnuba sworg tah ehW ,bajnuP fo secalp ydnas ,hsedarP rattU nretse
R dna nahtsaja tI .tsaoc aes gnola yabmoB a sis ,breh ekil eert ylerar ro ,burh ; era srewolf
5 ,lauxesib - cihpromonitca ,suorem ; denioj slapesylno esab sa ; ni nworg si tI .suolatepmys alloroc
eht fo htnom eht rebmetpeS . ]01[ si tnalp ehT
t desu yllanoitidar sa itna ,ciseglana -
a ,cilobana ,yrotammalfni tnegnirts dnaevitaxal . ]11[ setebaid ni desu osla si tI dnaamezce . ]21[ yllacifitneics neeb sah gurd ehT
e lacigolocamrahp niatrec rof detadilav ekil stceffromutitna ,31[ ]41 a lairetcabitn laiborcimitna dna ]51[ ,
itna - evitarefilorp ]61[ , h cimedipilopy dna a citorelcsorehtaitn fo rebmun A .
sa hcus stneutitsnocotyhp olakla β ,sdi - ,loretsotisf ,sedisocylg yxordyhylop dna sdionoval
p nanger e sedisocylg [ ]11 morf detalosi neeb evah.tnalp eht
.2 SDOHTEM DNA SLAIRETAM 1.2 lairetam tnalP
eht fo tnalp elohW yP ainedatpeL acinhcetor saw morf 0102 rebotcO fo htnom eht ni detcelloc fo noitacifitnedi lacimonoxat ehT .aidnI ,rupiaJ
.rD yb enod saw tnalp eht iniaS .C .D labriB ,wonkcuL ,ynatoboealaP fo etutitsnI inhaS .aidnI ,
ni detisoped saw 12711 :on nemiceps rehcuoV nemiceps ehT .wonkcuL ,PISB fo muirabreh eht fo tnemtraped eht ni devreserp osla si egelloC reevahaM reknahtreeT ,ysongocamrahP ,ytisrevinU reevahaM reknahtreeT ,ycamrahP fo
.dabadaroM 2.2 fo noitaraperP stcartxe
dna deirD dwop e der saw lairetam tnalp ni stnevlos suoirav htiw detcartxe ylevisseccus ,rehte muelortep ekil ytiralop fo redro gnisaercni %05 dna lonahtem ,etateca lyhte ,mroforolhc erew stcartxe deniatbo ehT .lohocla suoeuqa
uucav ni detartnecnoc ot rotaropave yrator m.eudiser dilosimes deniatbo
3.2 gnineercS lacimehcotyhP suoirav fo ecneserp eht enimreted ot redro nI ,diolakla ekil spuorg lacimehcotyhp ,sdica onima ,snietorp ,sdionovalf ,etardyhobrac ,sdiorets dna sedisocylg ,sninnat ,slonehp
erp demrofrep erew stset lacimehcotyhp yranimilstcartxe tnalp tnereffid no . ]91 ,81 ,71[
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 93 ]
4.2 tnemtaert dna slaminA oniblA ssiwS erew xes rehtie fo )g 5 ± 52( ecim
rednu segac etarapes ni ruof fo puorg a ni desuoh 2 ± 22( erutarepmet fo snoitidnoc dellortnoc C˚ .)
nedloG( teid dradnats nevig erew slamina llA retaw dna )ihleD weN ,deef mutibilda ehT .
elcanniP eht ni demrofrep saw yduts lamina ni aidnI ,lapohB ,etutitsnI hcraeseR lacidemoiB ,ysongocamrahp fo tnemtraped htiw noitaicossa
egelloC reevahaM reknahtreeT ,ycamrahP fo .dabadaroM ,ytisrevinU reevahaM reknahtreeT devorppa saw yduts tneserp eht rof locotorp ehT eettimmoC lacihtE laminA lanoitutitsnI morf rebmun lavorppa dna lapohB ,IRBP fo )CEAI(
.331/CEAI/IRBP saw 5.2 setebaiD decudnI naxollA
ecim onibla ssiwS erew )mg 5±52( xes rehtie fo ,dradnats ,lortnoc .e.i spuorg ruof ot ni dedivid
fo tnalp elohw fo tcartxe cilonahtem fo tceffE :1 elbaT acinhcetoryP ainedatpeL doolb gnitsaf no
ecim citebaid ni level esoculg . spuorG syaD
0 7 41 12 lortnoC 59.5±66.77 95.6±33.28 02.3±33.77 94.6±61.87
)gk/gm 01( dradnatS 39.91±38.813 67.42±61.152 44.32±33.691 88.71±66.331
)gk/gm 051( naxollA 24.81±5.713 29.91±61.133 56.91±61.593 64.22±5.463
)gk/gm002( puorg tseT 17.71±61.813 58.32±33.972 13.22±38.902 28.61±271
yb AVONA yaw eno gnisu yb enod saw sisylana lacitsitatS .puorg hcae ni star 6 rof .M.E.S ± naem sa nevig seulaV llA
t s’tennuD - sv ecnacifingis lacitsitatS detneserpeR ***,ecnacifingis fo level a sa deredisnoc saw 50.0 <p tset <p( lortnoc
)1000.0
fo tcartxe cilonahtem fo level esoculg doolb gniwohs hparG :1 .giF acinhcetoryp ainedatpeL elohw foecim citebaid decudni naxollA ni tnalp .
0
05
001
051
002
052
003
053
004
yaD 0 yaD 7
)ld/gm( leveL esocul
G doolB
syaD fo rebmuN
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 93 ]
4.2 tnemtaert dna slaminA oniblA ssiwS erew xes rehtie fo )g 5 ± 52( ecim
rednu segac etarapes ni ruof fo puorg a ni desuoh 2 ± 22( erutarepmet fo snoitidnoc dellortnoc C˚ .)
nedloG( teid dradnats nevig erew slamina llA retaw dna )ihleD weN ,deef mutibilda ehT .
elcanniP eht ni demrofrep saw yduts lamina ni aidnI ,lapohB ,etutitsnI hcraeseR lacidemoiB ,ysongocamrahp fo tnemtraped htiw noitaicossa
egelloC reevahaM reknahtreeT ,ycamrahP fo .dabadaroM ,ytisrevinU reevahaM reknahtreeT devorppa saw yduts tneserp eht rof locotorp ehT eettimmoC lacihtE laminA lanoitutitsnI morf rebmun lavorppa dna lapohB ,IRBP fo )CEAI(
.331/CEAI/IRBP saw 5.2 setebaiD decudnI naxollA
ecim onibla ssiwS erew )mg 5±52( xes rehtie fo ,dradnats ,lortnoc .e.i spuorg ruof ot ni dedivid
fo tnalp elohw fo tcartxe cilonahtem fo tceffE :1 elbaT acinhcetoryP ainedatpeL doolb gnitsaf no
ecim citebaid ni level esoculg . spuorG syaD
0 7 41 12 lortnoC 59.5±66.77 95.6±33.28 02.3±33.77 94.6±61.87
)gk/gm 01( dradnatS 39.91±38.813 67.42±61.152 44.32±33.691 88.71±66.331
)gk/gm 051( naxollA 24.81±5.713 29.91±61.133 56.91±61.593 64.22±5.463
)gk/gm002( puorg tseT 17.71±61.813 58.32±33.972 13.22±38.902 28.61±271
yb AVONA yaw eno gnisu yb enod saw sisylana lacitsitatS .puorg hcae ni star 6 rof .M.E.S ± naem sa nevig seulaV llA
t s’tennuD - sv ecnacifingis lacitsitatS detneserpeR ***,ecnacifingis fo level a sa deredisnoc saw 50.0 <p tset <p( lortnoc
)1000.0
fo tcartxe cilonahtem fo level esoculg doolb gniwohs hparG :1 .giF acinhcetoryp ainedatpeL elohw foecim citebaid decudni naxollA ni tnalp .
yaD 51 yaD 12
syaD fo rebmuN
lortnoC
)gk/gm01edimalcnebilG( dradnatS
)gk/gm 051( naxollA
)gk/gm 002( puorg tseT
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 93 ]
4.2 tnemtaert dna slaminA oniblA ssiwS erew xes rehtie fo )g 5 ± 52( ecim
rednu segac etarapes ni ruof fo puorg a ni desuoh 2 ± 22( erutarepmet fo snoitidnoc dellortnoc C˚ .)
nedloG( teid dradnats nevig erew slamina llA retaw dna )ihleD weN ,deef mutibilda ehT .
elcanniP eht ni demrofrep saw yduts lamina ni aidnI ,lapohB ,etutitsnI hcraeseR lacidemoiB ,ysongocamrahp fo tnemtraped htiw noitaicossa
egelloC reevahaM reknahtreeT ,ycamrahP fo .dabadaroM ,ytisrevinU reevahaM reknahtreeT devorppa saw yduts tneserp eht rof locotorp ehT eettimmoC lacihtE laminA lanoitutitsnI morf rebmun lavorppa dna lapohB ,IRBP fo )CEAI(
.331/CEAI/IRBP saw 5.2 setebaiD decudnI naxollA
ecim onibla ssiwS erew )mg 5±52( xes rehtie fo ,dradnats ,lortnoc .e.i spuorg ruof ot ni dedivid
fo tnalp elohw fo tcartxe cilonahtem fo tceffE :1 elbaT acinhcetoryP ainedatpeL doolb gnitsaf no
ecim citebaid ni level esoculg . spuorG syaD
0 7 41 12 lortnoC 59.5±66.77 95.6±33.28 02.3±33.77 94.6±61.87
)gk/gm 01( dradnatS 39.91±38.813 67.42±61.152 44.32±33.691 88.71±66.331
)gk/gm 051( naxollA 24.81±5.713 29.91±61.133 56.91±61.593 64.22±5.463
)gk/gm002( puorg tseT 17.71±61.813 58.32±33.972 13.22±38.902 28.61±271
yb AVONA yaw eno gnisu yb enod saw sisylana lacitsitatS .puorg hcae ni star 6 rof .M.E.S ± naem sa nevig seulaV llA
t s’tennuD - sv ecnacifingis lacitsitatS detneserpeR ***,ecnacifingis fo level a sa deredisnoc saw 50.0 <p tset <p( lortnoc
)1000.0
fo tcartxe cilonahtem fo level esoculg doolb gniwohs hparG :1 .giF acinhcetoryp ainedatpeL elohw foecim citebaid decudni naxollA ni tnalp .
)gk/gm01edimalcnebilG( dradnatS
)gk/gm 051( naxollA
)gk/gm 002( puorg tseT
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 04 ]
0
02
04
06
08
001
021
041
061
081
lortnoC dradnatS
801
61.4
8
L/UI
detaert tcartxe cilonahtem ,lortnoc citebaidculg doolb ,taht retfA .spuorg saw level eso
yb enO oculG( retemoculg latigid yb derusaem 051( etardyhonom naxollA .)nehproM .rD
artni yb ecim eseht ot deretsinimda saw )gk/gm laenotirep )pi( nO .)VI & III ,II puorG( noitcejni
dradnats ,yad driht eht sgurd tset dna )gk/gm 01(002( )gk/gm dna yllaro deretsinimda erew
12 rof lamron tog level esoculg eht llit deunitnoc no derusaem saw level esoculg doolb ehT .syad
saw doolb eht ,taht retfA .yad 12 dna 41 ,7 ,1 gnitamitse rof erutcnup latibro orter yb detcelloc
S ,TPGS ekil sretemarap suoirav ,TOG edirecylgirt dna loretselohc ]02[ .
6.2 sisylana lacitsitatS ehT .MES ± naem sa desserpxe era seulav ehT ecnacifingis lacitsitats rof dezylana erew stluser
eno gnisu - dewollof ,AVONA yaw s’tennuD ybt- .tnacifingis deredisnoc saw 50.0<P .tset
fo tcartxe cilonahtem fo tceffE :2 elbaT p ainedatpeL acinhcetory sretemarap lacimehcoib no puorG retemaraP lacimehcoiB
)L/UI( TPGS )L/UI( TOGS loretselohC )ld/gm( sedirecylgirT )ld/gm(
lortnoC 37.2±5.83 79.3±38.04 54.11±801 65.7±61.48
)gk/gm 01( dradnatS 90.2±44 71.5±74 97.11±5.211 51.6±66.59
)gk/gm 051( naxollA 18.5±66.97 5.4±33.88 66.7±871 58.41±66.471
)gk/gm002( puorg tseT 98.5±5.05 27.7±33.65 22.7±61.221 82.9±33.701 hcae ni star 6 rof .M.E.S ± naem sa nevig seulaV llA yb AVONA yaw eno gnisu yb enod saw sisylana lacitsitatS .puorg
000.0 <p( lortnoc sv ecnacifingis lacitsitats detneserpeR ***.ecnacifingis fo level a sa deredisnoc saw 50.0 <p tset s’tennuD )1
.giF fo tcartxe cilonahtem fo tceffe gniwohs hparG :2 acinhcetoryp ainedatpeL no tnalp elohw fo
ecim citebaid decudni naxollA tsniaga slamina detaert ni slevel TOGS dna TPGS .
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 04 ]
dradnatS naxollA puorg tseT
5.21
1
871
61.2
21
66.5
9
66.4
71
33.7
01
spuorG fo rebmuN
detaert tcartxe cilonahtem ,lortnoc citebaidculg doolb ,taht retfA .spuorg saw level eso
yb enO oculG( retemoculg latigid yb derusaem 051( etardyhonom naxollA .)nehproM .rD
artni yb ecim eseht ot deretsinimda saw )gk/gm laenotirep )pi( nO .)VI & III ,II puorG( noitcejni
dradnats ,yad driht eht sgurd tset dna )gk/gm 01(002( )gk/gm dna yllaro deretsinimda erew
12 rof lamron tog level esoculg eht llit deunitnoc no derusaem saw level esoculg doolb ehT .syad
saw doolb eht ,taht retfA .yad 12 dna 41 ,7 ,1 gnitamitse rof erutcnup latibro orter yb detcelloc
S ,TPGS ekil sretemarap suoirav ,TOG edirecylgirt dna loretselohc ]02[ .
6.2 sisylana lacitsitatS ehT .MES ± naem sa desserpxe era seulav ehT ecnacifingis lacitsitats rof dezylana erew stluser
eno gnisu - dewollof ,AVONA yaw s’tennuD ybt- .tnacifingis deredisnoc saw 50.0<P .tset
fo tcartxe cilonahtem fo tceffE :2 elbaT p ainedatpeL acinhcetory sretemarap lacimehcoib no puorG retemaraP lacimehcoiB
)L/UI( TPGS )L/UI( TOGS loretselohC )ld/gm( sedirecylgirT )ld/gm(
lortnoC 37.2±5.83 79.3±38.04 54.11±801 65.7±61.48
)gk/gm 01( dradnatS 90.2±44 71.5±74 97.11±5.211 51.6±66.59
)gk/gm 051( naxollA 18.5±66.97 5.4±33.88 66.7±871 58.41±66.471
)gk/gm002( puorg tseT 98.5±5.05 27.7±33.65 22.7±61.221 82.9±33.701 hcae ni star 6 rof .M.E.S ± naem sa nevig seulaV llA yb AVONA yaw eno gnisu yb enod saw sisylana lacitsitatS .puorg
000.0 <p( lortnoc sv ecnacifingis lacitsitats detneserpeR ***.ecnacifingis fo level a sa deredisnoc saw 50.0 <p tset s’tennuD )1
.giF fo tcartxe cilonahtem fo tceffe gniwohs hparG :2 acinhcetoryp ainedatpeL no tnalp elohw fo
ecim citebaid decudni naxollA tsniaga slamina detaert ni slevel TOGS dna TPGS .
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 04 ]
TPGS
TOGS
detaert tcartxe cilonahtem ,lortnoc citebaidculg doolb ,taht retfA .spuorg saw level eso
yb enO oculG( retemoculg latigid yb derusaem 051( etardyhonom naxollA .)nehproM .rD
artni yb ecim eseht ot deretsinimda saw )gk/gm laenotirep )pi( nO .)VI & III ,II puorG( noitcejni
dradnats ,yad driht eht sgurd tset dna )gk/gm 01(002( )gk/gm dna yllaro deretsinimda erew
12 rof lamron tog level esoculg eht llit deunitnoc no derusaem saw level esoculg doolb ehT .syad
saw doolb eht ,taht retfA .yad 12 dna 41 ,7 ,1 gnitamitse rof erutcnup latibro orter yb detcelloc
S ,TPGS ekil sretemarap suoirav ,TOG edirecylgirt dna loretselohc ]02[ .
6.2 sisylana lacitsitatS ehT .MES ± naem sa desserpxe era seulav ehT ecnacifingis lacitsitats rof dezylana erew stluser
eno gnisu - dewollof ,AVONA yaw s’tennuD ybt- .tnacifingis deredisnoc saw 50.0<P .tset
fo tcartxe cilonahtem fo tceffE :2 elbaT p ainedatpeL acinhcetory sretemarap lacimehcoib no puorG retemaraP lacimehcoiB
)L/UI( TPGS )L/UI( TOGS loretselohC )ld/gm( sedirecylgirT )ld/gm(
lortnoC 37.2±5.83 79.3±38.04 54.11±801 65.7±61.48
)gk/gm 01( dradnatS 90.2±44 71.5±74 97.11±5.211 51.6±66.59
)gk/gm 051( naxollA 18.5±66.97 5.4±33.88 66.7±871 58.41±66.471
)gk/gm002( puorg tseT 98.5±5.05 27.7±33.65 22.7±61.221 82.9±33.701 hcae ni star 6 rof .M.E.S ± naem sa nevig seulaV llA yb AVONA yaw eno gnisu yb enod saw sisylana lacitsitatS .puorg
000.0 <p( lortnoc sv ecnacifingis lacitsitats detneserpeR ***.ecnacifingis fo level a sa deredisnoc saw 50.0 <p tset s’tennuD )1
.giF fo tcartxe cilonahtem fo tceffe gniwohs hparG :2 acinhcetoryp ainedatpeL no tnalp elohw fo
ecim citebaid decudni naxollA tsniaga slamina detaert ni slevel TOGS dna TPGS .
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 14 ]
002040608001021041061081
lortnoC dradnatS
801
61.4
8
ld/gm
:3 .giF fo tcartxe cilonahtem fo tceffe gniwohs hparG acinhcetoryp ainedatpeL dna loretselohC no tnalp elohw fo tsniaga slamina detaert ni slevel sedirecylgirT citebaid decudni naxollA ecim
.3 STLUSER
fo esod a ta tcartxe cilonahtem fo noitartsinimdA fo gk/gm 002 acinhcetoryp ainedatpeL
itcuder desuac yltnacifingis doolb detavele ni no271 yb level esoculg saw tceffe eht tub ;28.61±
gurd dradnats fo taht naht ssel eb ot dnuof.)88.71±66.331( edimalcnebilg
eht derewol PLEM dna dradnats ,noitidda nI TPGS .e.i yltnacifingis sretemarap lacimehcoib
S ,)98.5±5.05 & 90.2±44( & 71.5±74( TOG & 97.11±2.211( loretselohC ,)27.7±33.65 & 51.6±66.59( sedirecylgirT dna )22.7±61.221
ylevitcepser )82.9±33.701
.4 NOISSUCSID gniylrednu msinahcem latnemadnuf ehT
revo sevlovni aimecylgrepyh - noitcudorp eht yb esoculg fo noitazilitu desaerced seussit
]32,22[ nommoc eht ,aimecylgrepyh tnetsisreP . citebaid tsom esuac nac setebaid fo citsiretcarahc dluohs tnemtaert ,stneitap lla nI .snoitacilpmoc lamron raen ot esoculg doolb rewol ot mia
cimeacylgomron eht ,noitagitsevni ruo nI .slevel
laro dna delaever seiduts tset ecnarelot esoculg fo tnalp elohw fo tcartxe cilonahtem eht taht
acinhcetoryp ainedatpeL ot yticapac eht sah citebaid ehT .slevel esoculg doolb rewol si naxollA htiw deretsinimda star ni emordnys
repyh etaredom elbats yb deziretcarahc ,aimecylg tnacifingis tub deretla dna ecnarelotni esoculg nI .noiterces nilusni detalumits esoculg eht taht setacidni atad eht ,yduts cimeacylgomron
fo tcartxe cilonahtem acinhcetoryP ainedatpeL esoculg doolb eht decuder yltnacifingis tnemtaert
t ni slevel lamron eht sdrawot star citebaid eh ehT.doirep yduts fo syad 12 eht ni level fo level eht sesaerced osla tcartxe cilonahtem & 90.2±44( TPGS ekil sretemarap lacimehcoib ,)27.7±33.65 & 71.5±74( TOGS ,)98.5±5.05 dna )22.7±61.221 & 97.11±2.211( loretselohC
irT & )82.9±33.701 & 51.6±66.59( sedirecylg )gk/gm 01 edimalcnebilG( gurd dradnatS lacimehcoib fo slevel eht decuder yltnacifingis TOGS ,)90.2±0.44( TPGS ekil sretemarap dna )97.11±5.211( loretselohC ,)71.5±0.74(
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 14 ]
dradnatS naxollA puorg tseT
5.21
1
871
61.2
21
66.5
9
66.4
71
33.7
01
spuorG fo rebmuN
:3 .giF fo tcartxe cilonahtem fo tceffe gniwohs hparG acinhcetoryp ainedatpeL dna loretselohC no tnalp elohw fo tsniaga slamina detaert ni slevel sedirecylgirT citebaid decudni naxollA ecim
.3 STLUSER
fo esod a ta tcartxe cilonahtem fo noitartsinimdA fo gk/gm 002 acinhcetoryp ainedatpeL
itcuder desuac yltnacifingis doolb detavele ni no271 yb level esoculg saw tceffe eht tub ;28.61±
gurd dradnats fo taht naht ssel eb ot dnuof.)88.71±66.331( edimalcnebilg
eht derewol PLEM dna dradnats ,noitidda nI TPGS .e.i yltnacifingis sretemarap lacimehcoib
S ,)98.5±5.05 & 90.2±44( & 71.5±74( TOG & 97.11±2.211( loretselohC ,)27.7±33.65 & 51.6±66.59( sedirecylgirT dna )22.7±61.221
ylevitcepser )82.9±33.701
.4 NOISSUCSID gniylrednu msinahcem latnemadnuf ehT
revo sevlovni aimecylgrepyh - noitcudorp eht yb esoculg fo noitazilitu desaerced seussit
]32,22[ nommoc eht ,aimecylgrepyh tnetsisreP . citebaid tsom esuac nac setebaid fo citsiretcarahc dluohs tnemtaert ,stneitap lla nI .snoitacilpmoc lamron raen ot esoculg doolb rewol ot mia
cimeacylgomron eht ,noitagitsevni ruo nI .slevel
laro dna delaever seiduts tset ecnarelot esoculg fo tnalp elohw fo tcartxe cilonahtem eht taht
acinhcetoryp ainedatpeL ot yticapac eht sah citebaid ehT .slevel esoculg doolb rewol si naxollA htiw deretsinimda star ni emordnys
repyh etaredom elbats yb deziretcarahc ,aimecylg tnacifingis tub deretla dna ecnarelotni esoculg nI .noiterces nilusni detalumits esoculg eht taht setacidni atad eht ,yduts cimeacylgomron
fo tcartxe cilonahtem acinhcetoryP ainedatpeL esoculg doolb eht decuder yltnacifingis tnemtaert
t ni slevel lamron eht sdrawot star citebaid eh ehT.doirep yduts fo syad 12 eht ni level fo level eht sesaerced osla tcartxe cilonahtem & 90.2±44( TPGS ekil sretemarap lacimehcoib ,)27.7±33.65 & 71.5±74( TOGS ,)98.5±5.05 dna )22.7±61.221 & 97.11±2.211( loretselohC
irT & )82.9±33.701 & 51.6±66.59( sedirecylg )gk/gm 01 edimalcnebilG( gurd dradnatS lacimehcoib fo slevel eht decuder yltnacifingis TOGS ,)90.2±0.44( TPGS ekil sretemarap dna )97.11±5.211( loretselohC ,)71.5±0.74(
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 14 ]
loretselohC
sedirecylgirT
:3 .giF fo tcartxe cilonahtem fo tceffe gniwohs hparG acinhcetoryp ainedatpeL dna loretselohC no tnalp elohw fo tsniaga slamina detaert ni slevel sedirecylgirT citebaid decudni naxollA ecim
.3 STLUSER
fo esod a ta tcartxe cilonahtem fo noitartsinimdA fo gk/gm 002 acinhcetoryp ainedatpeL
itcuder desuac yltnacifingis doolb detavele ni no271 yb level esoculg saw tceffe eht tub ;28.61±
gurd dradnats fo taht naht ssel eb ot dnuof.)88.71±66.331( edimalcnebilg
eht derewol PLEM dna dradnats ,noitidda nI TPGS .e.i yltnacifingis sretemarap lacimehcoib
S ,)98.5±5.05 & 90.2±44( & 71.5±74( TOG & 97.11±2.211( loretselohC ,)27.7±33.65 & 51.6±66.59( sedirecylgirT dna )22.7±61.221
ylevitcepser )82.9±33.701
.4 NOISSUCSID gniylrednu msinahcem latnemadnuf ehT
revo sevlovni aimecylgrepyh - noitcudorp eht yb esoculg fo noitazilitu desaerced seussit
]32,22[ nommoc eht ,aimecylgrepyh tnetsisreP . citebaid tsom esuac nac setebaid fo citsiretcarahc dluohs tnemtaert ,stneitap lla nI .snoitacilpmoc lamron raen ot esoculg doolb rewol ot mia
cimeacylgomron eht ,noitagitsevni ruo nI .slevel
laro dna delaever seiduts tset ecnarelot esoculg fo tnalp elohw fo tcartxe cilonahtem eht taht
acinhcetoryp ainedatpeL ot yticapac eht sah citebaid ehT .slevel esoculg doolb rewol si naxollA htiw deretsinimda star ni emordnys
repyh etaredom elbats yb deziretcarahc ,aimecylg tnacifingis tub deretla dna ecnarelotni esoculg nI .noiterces nilusni detalumits esoculg eht taht setacidni atad eht ,yduts cimeacylgomron
fo tcartxe cilonahtem acinhcetoryP ainedatpeL esoculg doolb eht decuder yltnacifingis tnemtaert
t ni slevel lamron eht sdrawot star citebaid eh ehT.doirep yduts fo syad 12 eht ni level fo level eht sesaerced osla tcartxe cilonahtem & 90.2±44( TPGS ekil sretemarap lacimehcoib ,)27.7±33.65 & 71.5±74( TOGS ,)98.5±5.05 dna )22.7±61.221 & 97.11±2.211( loretselohC
irT & )82.9±33.701 & 51.6±66.59( sedirecylg )gk/gm 01 edimalcnebilG( gurd dradnatS lacimehcoib fo slevel eht decuder yltnacifingis TOGS ,)90.2±0.44( TPGS ekil sretemarap dna )97.11±5.211( loretselohC ,)71.5±0.74(
ramuK S la te :. ytivitca citebaiditnA .htem fo fo .txe acinhcetoryp ainedatpeL enceD ).ksroF(
,nokamrahP atcA 1( .loV ,1 eussI ,) yraunaJ - 5102 hcraM [ 24 ]
)51.6±66.59( sedirecylgirT ehT .ylevitcepserih tnemtaert taht dewohs yduts lacigolohtapots
eht fo tcartxe cilonahtem htiw ainedatpeL
acinhcetoryp ,sisorbif fo secnedive on gniwohs noitces ssorc dna sllec lanogylop fo .on decuder
.seirallipac doolb fo .
SECNEREFER
.1 raraB ,scitueparehtocamrahP fo laitnessE .SF ,ynapmoC dna dnahC .S ,ihleD weN .04 .p .4002
.2 5 .ygolocamrahP .KP erooM ,MJ rettiR ,MM elaD ,PH gnaR ht :notsgniviL llihcruhC ,grubnidE .de583 .p .3002 ;dtL ecneicS reiveslE - .6
.3 s’namliG dna namdooG .LE eeL ,HG leoJ - ,kroY weN .scituepareht fo sisab lacigolocamrahP ehTwarGcM - 6861.p ,1002 ;noisiviD gnihsilbuP lacideM lliH - .7
.4 warGcM ,ASU .ygolocamrahP lacinilC dna cisaB .GB gnuztaK - 396 .p ,4002 ;seinapmoc lliH - .4
.5 .ygolocamrahP .SS erupaniA ,DS rakradnahB ,SR raksotaS .p ,1002 ;nahsakarp ralupoP ,iabmuM498 - .31
.6 .sutillem setebaid no eettimmoc trepxe OHW eht fo troper dnoceS .noitazinagrO htlaeH dlroW.66 ,0891.p ;aveneG ,noitazinagrO htlaeH dlroW
.7 RP ttalF ,MA yarG )7991( . rtuN J rB ,87 ,avitaS ogacideM , 523 - .43
.8 aS ,X uH Y otaS ,G ottojaB ,M uX ,Y adihsO ,J ot .)3002( tcarP nilC seR setebaiD , 95 , 301 - .11
.9 P redmujoM ,KS irudahaB ( 8002 ) . ecnaidaR dorP taN , .95 .01 AM helaS ,IA riadohK ,MA afatsuoM fessuoY ( 7002 ) . locamrahP J , 186 - .79 .11 K niaJ ,S lawragA ,S inalahJ ,CG niaJ )7002( . icS pxE J naisA , 511 - .22 .21 KP valaG ,SS awetaK ( 6002 ) . wonK tidarT J naidnI , 494 - .005 .31 FM iabeS ,AF airdaB ,AA lamaG ,AM nassaH ,SM ififA ( 2002 ) . icS mrahP J aruosnaM , 221 - .83 .41 AM helaS ,IA riadohK ,MA afatsuoM fessuoY ( 9002 ) . loiB mrahP , 628 - .43 .51 B ,D tolheG A arho ( 2002 ) . icS tnalP vdA , 52 - .7 .61 M isammoT ,SG occozraM ,AF zaiP laD ,A ollassaV ,R ogonoS ,G iffoiC ( 6002 ) . dorP taN J ,
526 - .53 .71 ysongocamrahP lacitcarP .RK lawlednahK - ;nahsakarp ilariN :enuP .stnemirepxe dna seuqinhcet
941 .P .2002 - .65 .81 arP .KC etakoK eD weN .ysongocamrahP lacitc .P .5002 ;nahsakarp hballaV :ihl 701 - .31 .91 91 .p .6002 ;ycnega gnihsilbup aidnI weN :ihleD weN .seuqinhceT lacimehcotyhP .N namaR - .42 .02 M anitsirhC ,R iahtoK ,B lurA ( 6002 ) . seR mrahP J naidnI , 74 - .15 .12 KA yrahduahC ,V hgniS ,R iveD ( 0102 ) . seR veR icS mrahP J naidnI , 78 - .29 .22 .84 ;8591 aihpledalihP :srednuaS .yrtsimehcoiB lacinilC .A rentaL .32 G arhsiM ,LR asohK ,P hgniS ( 3102 ) . icsoiB nilC pxE J regiN ,1 , 33 - .8
