Speicher DNA (Gen) ATG.CTC.. Botschaft mRNA AUG.CUC … Produkt Metabolit A Protein (Enzym)...
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Transcript of Speicher DNA (Gen) ATG.CTC.. Botschaft mRNA AUG.CUC … Produkt Metabolit A Protein (Enzym)...
Speicher
DNA(Gen)ATG.CTC..
Botschaft
mRNA
AUG.CUC…
Produkt Metabolit A
Protein(Enzym) Metabolit BMet.Leu...
Informationsfluss Stofffluss
5000 4000 4000 2000 Genom Transkriptom Proteom Metabolom
Abb.1
RRegulator
144 aa
PPromotor
T PTransport
Peri-plasma116 aa
TransportMembrane
91 aa
AMercury-
Reductase561 aa
BMercury-
Lyase212 aa
Hg(II)
Abb.2
Secretion Degradation
RG Promotor Operator TF Struc. DNA
mRNA
EnzymeMetabolite P
Transcription
TranslationInhibition
Degradation
Apo-repressor
Repressor
Feedbackrepression
Operon
Abb. 3
idijiFiii
i mRNAkcccTODdt
dmRNA ..,..,)( 21
Transkription
isijiii
i EkcccmRNAdtdE
...,...., 21
Translation
...),,...,( 121 jjii ccccErij Metabolische Flüsse
GEN
PRODUKTdcd t
r cjij
i
n
j
Abb.4
40 45 50 55 60 65 70 750
2
4
6
t3
t2
Phase IIPhase IC
B
A
Tryp
toph
an
Time
6
7
8
9 Phase IIPhase It1
Enzy
me
0,00,30,60,9
mRN
A
Abb. 5
Abb. 6
MALSYTSJ
AroBAroCAroDAroIAroE
AroAAroF
AspBCitZ
ASPOBUT
THR
ILE
TKT2 TAL
RPE
GLUC
3PG
G3P
F6P
PEP
PYR AcCoA
G6P
E4P
X5P
SER
GLY
Rib5P
R5P
CHOR PHEN
TYR
PHE
VAL
GLK
PGI
PFKFBATPI
PGMENO
PYK
ZWF GntZ
RPI
PdhA PdhBAcAcCoA
MmgA
GlyA
TKT
AroA
IlvA
SerB SerC SerA
GLU
GLN
MDH
OAA CIT
AKGMAL
SUCCFUMA
CitBCitC
OdhBSucCSucDFumA
GlnA
GAPPGK
PtsI
PycA
SdhA
GltB
YclMASDHOMKhsE
IlvCIlvDIlvE
HOThPPOIVAIlvE IlvB
IlvCIlvD
ThrC
LEU
LeuALeuB
LeuCDIlvE
PdhC PdhD
AspB
AspBTyrA
PheA
CYS
CysECysK
MmgB
3-HBCoA
PHB
PHBSynthase
PTA
Acetyl-P
AckA
Acetate
Abb. 7
GenomeAnnotation
EnzymesEC 1.1.1.3EC 1.1.1.6
EC 1.1.1.17
Reversibility information
Connectionmatrix
C00013-C00009C00022-C00900C01083-C00267
Reactions Homoserine + NAD+ = Aspartate-Glycerol + NAD+ = Glycerone +Mannitol 1-P + NAD+ = F6P + N
…………………
Reaction database
(e.g. Ligand, Brenda
Nonenzymatic reactions
Ma & Zeng (2003) Bioinformatics. 19:1423-30
Reaction
Metabolite
Abb. 8
MethionineCysteine
sulfur
One carbon, folate, glycine, serine, threonine
TCA
Phenylalanine tyrosine tryptophan
Urea cycle, nitrogen, glutamate, arginine, proline, pyrimidine
Fatty acidValine, leucine
isoleucine
Pentose phosphate fructose, mannose galactose
Purine
Purinede novo
Glycerolipid, glycosphingolipid
Methane
Lysine
EC numbers: 434Metabolites: 1172
Glycolysis, pyruvate
Abb. 9
.
rpoE
fis
soxR
cspA
rpoS
phoB
crp
ihf
argP
cspE
rpoNdnaA
hnscytR
Global regulators
Regulatory modules
Abb. 10
Nahrung = 350 Signale
Gen mRNA Protein
A
B
Osmolarität pH-Wert Temperatur
Sauerstoff
Antibiotika
Druck
Licht
Erdanziehungskraft
Gift/Gefahr
Botschaften andererBakterien Scherstress
Nährstoff-limitierung
Abb. 11
Abb. 12
Abb, 13
Abb. 14
Shaking Flask
1µm
Couette flow bioreactor
= 0.028 s-1 = 1482 s-1
Abb. 15
Keimung2
50 µm8 Stunden
Pellet
Wachstum4
Sporen SporenpaketePrimäreAggregation
1
50 µm2 Stunden
SekundäreAggregation
3
100 µm12 Stunden
Abb. 16
Pelle
tkon
zent
ratio
n [1
/L]
Pelle
tdur
chm
esse
r [m
m]
0,1
1,0
10,0
104
105
106
0,050 0,060 0,070 0,080 0,090 0,100spezifischer Leistungseintrag [W/kg]
Akt
ivitä
t [µK
at/L
Rea
ktor
volu
men
]
0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
0
20
40
60
80
100
120
140
160
180
Akt
ivitä
t [µK
at/m
L Pe
lletv
olum
en]t = 80 h
Abb.17
Genom-Sequenzen
Proteom(Proteine)
Transkriptom(mRNA)
gezielte Exp.Metabolic Eng.
Zellphysiologie Kinetik
Metabolom Fluxom
Bioprozesse& Bioprodukte
EnvironomVerfahrenstechnik
metabolisches Netzwerk
genetisches Netzwerk
Abb. 18