Wister rat - env · 2014. 10. 16. · +T4 (10-8 M)-T4-T4, +BDE209 (10-10 M) +T4 (10-8 M), +BDE209...
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3. Purkinje 1 Wister rat , 17 carbindin Purkinje FITC - mouseIgG (n=10)
Transcript of Wister rat - env · 2014. 10. 16. · +T4 (10-8 M)-T4-T4, +BDE209 (10-10 M) +T4 (10-8 M), +BDE209...
3.
Purkinje
1 Wister rat ,
17
carbindin Purkinje
FITC - mouseIgG
(n=10)
+T4 (10-7 M) +T4 ( 10-6 M)
+T4 (10-9 M)+T4 (10-10 M)-T4
3. Purkinje :
+T4 (10-8 M)
T4 (M) - 10-10 10-9 10-8 10-7 10-6
Den
driti
car
ea (
%)
3. Purkinje :
+T4 (10-8 M)-T4
-T4, +BDE209
(10-10 M)
+T4 (10-8 M),
+BDE209 (10-10 M)R
elat
ive
dend
ritic
area
0
1
2
3
4
5
BDE209 - -+ +
T4 - - ++
3. Purkinje :
BDE209
Ibhazehiebo et al. Environ Health Perspect, 2010
Purkinje
1. Purkinje Purkinje
2.,
(1)(2) Ca2+
4.
(1)
7 Wister rat,
2
+T3 (10-7 M)+T3 (10-8 M)
+T3 (10-10 M)+T3 (10-11 M)
-T3
+T3 (10-6 M)
+T3 (10-9 M)
4.
T3 neurite
Ca2+ imaging: Fura2-AMCortex Primary culture
OHOH--PCB 106 0.1 PCB 106 0.1 μμMM 10 sec.10 sec.
20 sec.20 sec. 30 sec.30 sec. 40 sec.40 sec.
BASE LINEBASE LINE
5- Ca2+ –
0.1 ratio5 min
Type 1.1
0.1 ratio5 min
Type 1.2
0.8
0
0.2
0.4
0.6
-10 - 0 0 - 10 10 - 20 20 - 30 30 - 40
Period (min)
**
Type 1.2
0
**
**
0.2
0.4
0.6
0.8Type 1.1
-10 - 0 0 - 10 10 - 20 20 - 30 30 - 40
Period (min)
Type 1.3
0.25 ratio
5 min
Type 1.4
0.1 ratio5 min
Londono et al., J Appl Toxicol. 2010
OH-PCB106 Ca2+
5. Ca2+
Ca2+ (–) Ca2+ (+)A
0.1 ratio
5 min
Type 2.1
0.1 ratio
5 min
Type 2.2
0.1 ratio
5 min
Type 2.4
0.1 ratio
5 min
Type 2.3
B
80
60
40
20
02.1 2.2 2.3 2.4
Per
cent
age
of to
tal c
ells
%
Type
Londono M. et al., J Appl Toxicol. 2010
5. OH-PCB106 Ca2+ Ca2+
Purkinje 2+
OH-PCB
PBDE
HBCD
4-NP
TR
Ca2+
.
ExTEND 2005
CIN85 KO
1 GFP
GFP
2 in vivo
2 /
3) (ADHD)
Dopaminetransporter
Dopamine Receptors
Dopaminein vesicle
L-tyrosine
L-DOPA
TH
Dopadecarboxylase Dopamine
1.ADHD
2.
1.ADHD
2.
1. ADHD
2.
3.
1. ADHD
2.
3.
